Conformational Changes in Stratum Corneum Lipids by Effect of Bicellar Systems

Abstract

Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy was applied to study the effects of the bicelles formed by dimyristoyl-glycero-phosphocholine (DMPC) and dihexanoyl-glycero-phosphocholine (DHPC) in porcine stratum corneum (SC) in vitro. A comparison of skin samples treated and untreated with bicelles at different temperatures was carried out. The analysis of variations after treatment in the position of the symmetric CH2 stretching, CH2 scissoring, and CH2 rocking vibrations reported important information about the effect of bicelles on the skin. Bicellar systems caused a phase transition from the gel or solid state to the liquid crystalline state in the lipid conformation of SC, reflecting the major order-disorder transition from hexagonally packed to disordered chains. Grazing incidence small and wide X-ray scattering (GISAXS and GIWAXS) techniques confirmed this effect of bicelles on the SC. These results are probably related to with the permeabilizing effect previously described for the DMPC/DHPC bicelles.

Natural polymer-stabilized multiple water-in-oil-in-water emulsions: a novel dermal drug delivery system for 5-fluorouracil

“…The position of the band at 2851.36 ± 0.22/cm after impregnation of the porcine skin with the O/W‐macroemulsion suggested a dominating hexagonal phase, while the position of the symmetric CH 2 stretching mode at about 2852/cm after impregnation with the four multiple emulsions indicated dominating liquid disordered phases. The characteristic wave numbers of the different conformational orders of the alkyl chains of the stratum corneum lipids were reported by Rodríguez et al [24]…”

Grazing incidence wide angle x-ray scattering at the wiggler beamline BW4 of HASYLAB

“…Commonly investigated material systems include conjugated polymers, block copolymers, lipids, and magnetic or semiconducting materials in a wide variety of applications. [39][40][41][42][43][44][45][46] To establish and prove the feasibility of GIWAXS at BW4, we applied this technique to a variety of different samples. Here we present the sample of a thin film of the conjugated and conducting polymer poly͑3-octylthiophene͒ P3OT on top of a precleaned glass slide.…”

Bicelles: Lipid Nanostructured Platforms with Potential Dermal Applications

“…However, this was not observed, suggesting that these bicelles were not able to penetrate or disperse through the SC lipid area 40. This result was clarified by Rodriguez et al78 using attenuated total reflectance–Fourier transform infrared (ATR‐FTIR) spectroscopy. These authors reported that the application of DMPC/DHPC q = 2 bicelles caused phase transitions in the SC lipid conformation from the gel state to the liquid‐crystalline state.…”

“…Bicelles act to enhance penetration, causing phase transformations in lipid domains that may be relevant to skin permeation 92. As reported by Rodriguez et al,78 the phase transition of the SC lipid conformation from the gel state to the liquid‐crystalline state, which causes the fluidity of these lipids, explains the increase of TEWL in vivo. Compared to other enhancers, bicelles would have the additional advantage of not causing skin irritation 93…”