Conformational changes of fibronectin induced by polystyrene derivatives with a heparin‐like function

Abstract

It was previously reported that polystyrene substituted with the sulfonate group, PSSO3, which has anticoagulant heparin-like properties, and then coated with fibronectin supports the growth of human umbilical vein endothelial cells. On the other hand, polystyrene substituted with the amino acid sulfamide group, PSSO2-Asp, which has a higher anticoagulant activity, and then coated with fibronectin no longer supported the growth of endothelial cells. We report here that, while the affinity of fibronectin to either polymer is of the same order of magnitude, fibronectin is adsorbed onto the PSSO2-Asp polymer in a different conformation compared to the PSSO3 polymer. This was shown by a higher binding of polyclonal antifibronectin antibodies to fibronectin-coated PSSO2-Asp polymer, and by a decreased susceptibility of the coated fibronectin to proteolysis by thermolysin. This study provides evidence that a solid phase substrate with a strong heparin-like function may influence the conformation and biological properties of fibronectin.

Inhibition of lens epithelial cell proliferation by substituted PMMA intraocular lenses

“…These changes led to an increase in exposure of antigenic sites, while exposure of the cell attachment domain (HB10) remained unchanged. The authors concluded that the cell attachment domain of fibronectin was not involved in the decreased cell proliferation and that an additional domain could be implicated [14]. Due to a great number of binding domains fibronectin can interact with different ECM proteins such as collagen, fibrin, and heparin, and cell-surface receptors of the integrin family.…”

“…Stanislawski et al showed that substitution of polystyrene with the amino acid sulfamide group, PSSO 2 -Asp did not support cell growth in contrast to substitution of polystyrene with the sulfonate group, PSSO 3 -. They concluded that a polystyrene substrate grafted with different PSSO residues will absorb the ECM protein fibronectin into a conformation that is either favorable (PSSO 3 -) or unfavorable (PSSO 2 -Asp) for cell growth [14].…”

Biospecific Properties of Random Copolymers, 2

“…[3] The addition of sulfonate and carboxylic groups into a macromolecular chain according to specific compositions can cause a modulation of endothelial cell proliferation. [4,5] Full Paper: Previous results obtained have shown that it was possible to create biospecific sites onto polymers through statistic modifications of polystyrene derivatives copolymer with given chemical groups or through synthesis of random methacrylate-based copolymers. Such polymers show biological activities (anticoagulant, cell proliferation modulation .…”

Review Physicochemical and immunological basis of silicone pathophysiology

“…Evidence of molecular denaturation following surface adsorption of a broad range of biological macromolecules to various surfaces is readily available because it is a fundamental biophysical phenomenon [39,[45][46][47][48][49][50][51][52][53]. A recent study of fibronectin, a high molecular weight glycoprotein that is present in soluble form in plasma and in insoluble form in connective tissue and in basement membranes, is exemplary.…”

“…Stanislawsky et al [48] showed that when fibronectin adsorbs to different polymers, it may assume markedly different conformational states. In a series of elegant experiments, they showed that the amount of fibronectin adsorbing to sulfonate-derivitized polystyrene was comparable to the amount adsorbing to amino acid sulfamide-derivatized polystyrene.…”