Congenital Toxoplasmosis a Prospective Study of 378 Pregnancies

Desmonts, G; Couvreur, J

doi:10.1007/978-94-011-6621-8_24

Cited by 135 publications

(180 citation statements)

References 18 publications

Supporting

Mentioning

153

Contrasting

Unclassified

Order By: Relevance

“…53 Results of the present study support this epidemiologic evidence because it was observed that the number of parasites found at the trophoblast layer and mesenchyme was greater in third-than in first-trimester explants. Reproductive biologists have long been interested in differences of susceptibility to infection during gestation.…”

Section: Discussionsupporting

confidence: 86%

Effect of Macrophage Migration Inhibitory Factor (MIF) in Human Placental Explants Infected with Toxoplasma gondii Depends on Gestational Age

Gomes¹,

Silva²,

Silva³

et al. 2011

The American Journal of Pathology

View full text Add to dashboard Cite

Because macrophage migration inhibitory factor (MIF) is a key cytokine in pregnancy and has a role in inflammatory response and pathogen defense, the objective of the present study was to investigate the effects of MIF in first-and third-trimester human placental explants infected with Toxoplasma gondii. Explants were treated with recombinant MIF, IL-12, interferon-␥, transforming growth factor-␤1, or IL-10, followed by infection with T. gondii RH strain tachyzoites. Supernatants of cultured explants were assessed for MIF production. Explants were processed for morphologic analysis, immunohistochemistry, and real-time PCR analysis. Comparison of infected and stimulated explants versus noninfected control explants demonstrated a significant increase in MIF release in first-trimester but not third-trimester explants. Tissue parasitism was higher in third-than in first-trimester explants. Moreover, T. gondii DNA content was lower in first-trimester explants treated with MIF compared with untreated explants. However, in third-trimester explants, MIF stimulus decreased T. gondii DNA content only at the highest concentration of the cytokine. In addition, high expression of MIF receptor was observed in first-trimester placental explants, whereas MIF receptor expression was low in third-trimester explants. In conclusion, MIF was up-regulated and demonstrated to be important for control of T. gondii infection in first-trimester explants, whereas lack of MIF up-regulation in third-trimester placentas may be involved in higher susceptibility to infection at this gestational age.

show abstract

Section: Discussionsupporting

confidence: 86%

Effect of Macrophage Migration Inhibitory Factor (MIF) in Human Placental Explants Infected with Toxoplasma gondii Depends on Gestational Age

Gomes¹,

Silva²,

Silva³

et al. 2011

The American Journal of Pathology

View full text Add to dashboard Cite

show abstract

“…In this group 26 cases out of 80 (32.5%) were found to be positive for anti-toxoplasma IgG antibody which was significantly higher than that seen in the control group (P < 0.05). This finding is in agreement with various workers from India and abroad [1,[8][9][10][11]. In all studies the sero-prevalence was higher than the age matched controls.…”

Section: Discussionsupporting

confidence: 93%

Serological Diagnosis of Toxoplasma Gondii Infection in Various Patient Population in the Armed Forces

Praharaj

Singh

Chander³

et al. 2001

Medical Journal Armed Forces India

View full text Add to dashboard Cite

The study included 200 healthy age and sex matched controls (120 healthy volunteers and 80 healthy antenatal cases without any bad obstetric history) and 300 cases comprising patients with bad obstetric history (BOH) 80 cases, seizure or encephalitis 50 cases, cervical lymphadenopathy 30 cases, congenital deformity 30 cases, HIV infected 80 cases and patients on long term immunosuppression 30 cases. Anti-toxoplasma IgG & Igl\l were measured by ELISA and lndivlduals found positive for anti Toxoplasma gondii IgM or with high titre of IgG were subjected to repeat testing after 3 weeks for rise in titre. True sero-prevalence of T gondii infection was found to be 19% and it increased with increasing age in the population, 2.33% patients were found to have acute toxoplasma infection. Statistical significance was seen only in patients with BOH and children born with congenital abnormality. MJAFI 2001; 57: 298-301KEY WORDS: Bad obstetric history; Congenital abnormality; Serodiagnosis; Toxoplasmosis. 5 ml.of blood was collected and serum after separation was stored at -70 uC for testing. Serum was assayed for IgG and IgM antibody to T gondii by using commercial ELISA kits (United Biotech U.S.A). All the samples were tested in duplicate alongwith kit controls and the mean value of each sample was taken into consideration for calculating results. Manufacturer's instruction was followed for ELISA procedure. Serum samples from patients were re-tested for antitoxoplasma IgG antibody after 3 weeks if found positive for IgM isotype or with high titre of IgG antibody in the first testing. Statistical analysis was carried out by Chisquare test and odds ratio.

show abstract

“…Congenital toxoplasmosis may occur when seronegative women is infected during pregnancy. The risk of pregnant women being primarily infected with T. gondii is related to the general incidence of infection in the population and the infection rate in women in childbearing age 8 . Acute toxoplasmosis is generally acquired by oral route, mainly through the consomption of mammalian raw or undercooked meet infected with tissue cysts; fecal-oral route is caused by contamination of sporulated oocysts from cat faeces 10 .…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of toxoplasmosis in Fortaleza, Ceará, Brazil

Rey

Ramalho

1999

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

SUMMARYA serological survey of Toxoplasma gondii infection in population groups in Fortaleza, Brazil, was conducted, in order to identify seroprevalence rates according to age and risk factors associated with acquired infection. A Toxoplasma IgG-antibody enzyme immunoassay (Sanofi Pasteur Diagnostics, Marnes la Coquette, France) was employed to assess the immunity. Public day-care centers and schools were randomly selected, while three large antenatal clinics and maternity units were choosen by its importance. Population groups and results of 997 blood tests were: 227 children (mean age 3.8 years), 22.8% seropositives; 584 students (mean 11.4 years), 58.4%, and pregnant and postpartum women (mean 24 years), 71.5% seropositives (p < 0.001). Of 256 participants reporting close contact with cats, 59.8% were seropositive, in contrast with 51% seropositives without contact (relative risk 1.17; 95% confidence interval 1.04-1.33; p = 0.01). Having three or more siblings at home was related to higher seroprevalence in children and students (relative risk 1.39; 95% confidence interval 1.21-1.60; p < 0.01). In conclusion, toxoplasmosis seroprevalence showed a rapid increase during the first ten years of life, in association with close contact with cats and larger households, probably related to inappropriate hygiene and child-care practices.

show abstract

Congenital Toxoplasmosis a Prospective Study of 378 Pregnancies

Cited by 135 publications

References 18 publications

Effect of Macrophage Migration Inhibitory Factor (MIF) in Human Placental Explants Infected with Toxoplasma gondii Depends on Gestational Age

Effect of Macrophage Migration Inhibitory Factor (MIF) in Human Placental Explants Infected with Toxoplasma gondii Depends on Gestational Age

Serological Diagnosis of Toxoplasma Gondii Infection in Various Patient Population in the Armed Forces

Seroprevalence of toxoplasmosis in Fortaleza, Ceará, Brazil

Contact Info

Product

Resources

About