Conidiation under illumination enhances conidial tolerance of insect-pathogenic fungi to environmental stresses

Dias, Luciana Pereira Pinto; Souza, Roberta K.F.; Pupin, Breno; Rangel, Drauzio E.N.

doi:10.1016/j.funbio.2021.06.003

Cited by 27 publications

(4 citation statements)

References 74 publications

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“…This condition did not have a negative impact on the growth and virulence of S. lanosoniveum CG888. The conidia of this fungus have quite a good tolerance for production in light or dark conditions [19]. Apart from that, there have been no reported negative impacts on S. lanosoniveum to date.…”

Section: Resultsmentioning

confidence: 96%

Entomopathogenicity ofSimplicillium lanosoniveumCG888 on Mortality ofCallosobruchus maculatusF. in vitro

Susanti,

Hidayat,

Muhibuddin

2024

BIO Web Conf.

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Research regarding Simplicillium lanosoniveum as an entomopathogen is still limited. The entomopathogenicity of Simplicillium lanosoniveum CG888 with three different conidia density treatments, namely 1x106/ml, 1x107/ml, and 1x108/ml, was tested on Callosobruchus maculatus F. imago in the laboratory, incubated at 26-27°C, RH 70% and in the dark conditions. Based on the effect of conidia density, S. lanosoniveum CG888 at a density of 1x108 conidia/ml caused greater mortality of C. maculatus F, reaching 51.65% compared to other treatments and control. The influence of the length of time after inoculation was that there was 60.83% C. maculatus F. death on the 11th day. It was higher than the result on 3, 5, 7 and 9 days later. There was a positive interaction that the mortality of C. maculatus F was influenced by the density of S. lanosoniveum CG888 conidia and the period of days after inoculation. Among these treatments, S. lanosoniveum CG888 with 1x108/ml caused mortality of 86,7% in C. maculatus on day 11 after inoculation. The lowest mortality of C. maculatus F. was in the density of 1x106 conidia/ml of 70%. These findings indicate that S. lanosoniveum CG888 can be used to control C. maculatus F. safely and reduce the use of chemicals in soybean storage.

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Section: Resultsmentioning

confidence: 96%

Entomopathogenicity ofSimplicillium lanosoniveumCG888 on Mortality ofCallosobruchus maculatusF. in vitro

Susanti,

Hidayat,

Muhibuddin

2024

BIO Web Conf.

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“…To our knowledge, the effect of light on the genus Beauveria has so far only been explored in the species B. bassiana . All available studies on this subject highlight that this species is very light-responsive in terms of morphology, conidial production, stress tolerance, and metabolite production [ 25 – 28 , 57 ].…”

Section: Discussionmentioning

confidence: 99%

Light in the box—photobiological examination chamber with light trap ventilation system for studying fungal surface cultures illustrated with Metarhizium brunneum and Beauveria brongniartii

Vrabl

Zottele

Colleselli

et al. 2023

Fungal Biol Biotechnol

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Due to their versatile way of life as saprophytes, endophytes, and entomopathogens, fungi of the genera Metarhizium and Beauveria are exposed to varying illumination conditions in their natural habitats, which makes a thorough adaptation to light very likely. While the few available studies for these genera support this assumption, research in this field is still in its infancy and the data material restricted to only a few fungal species. Thus, the aim of this work was to explore how light influences growth, conidial production and secondary metabolite formation of two industrial relevant strains of M. brunneum (MA 43, formerly M. anisopliae var. anisopliae BIPESCO 5/F52) and B. brongniartii (BIPESCO 2). To achieve this, we constructed an easily adjustable illumination device for highly standardized photophysiological studies of fungi on Petri dishes, the so-called LIGHT BOX. With the aid of this device, M. brunneum and B. brongniartii were grown on S4G or S2G agar at 25 °C for 14 days either in complete darkness or under constant illumination with red light (λpeak = 635 nm), green light (λpeak = 519 nm) or blue light (λpeak = 452 nm). In addition, for each wavelength the effect of different illumination intensities was tested, i.e., intensities of red light ranging from 22.1 ± 0.1 to 136.5 ± 0.3 µW cm-2, green light from 16.5 ± 0.1 to 96.2 ± 0.1 µW cm-2, and blue light from 56.1 ± 0.2 to 188.9 ± 0.6 µW cm-2. Both fungi strongly responded in terms of growth, conidial production, pigmentation and morphology to changes in the wavelength and irradiation intensity. The wavelength-dependent production of the well-known secondary metabolite oosporein which is secreted by the genus Beauveria in particular, was also increased under green and blue light exposure. The established LIGHT BOX system allows not only to optimize conidial production yields with these biotechnologically relevant fungi, but also allows the photobiological exploration of other fungi.

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“…That is why conidia produced in blue light are more resistant to different abiotic stresses. Recently, Rangel et al showed that in the entomopathogenic fungi Lecanicillium aphanocladii and Simplicillium lanosoniveum, the effect of the nutritional stress on conidial resistance is stronger than that of illumination [31]. Conidia formed under nutritional stress are more resistant to several abiotic stresses than those formed in light.…”

Section: Discussionmentioning

confidence: 99%

“…Assessment of conidial resistance to abiotic stresses was performed as previously described by Dias et al with minor modifications [23,31]. Conidia produced in different wavelengths or light intensities were harvested as above and spore suspensions were diluted to a final concentration of 1 × 10 3 conidia/mL.…”

Section: Assessment Of Conidial Stress Resistancementioning

confidence: 99%

A Simple and Low-Cost Strategy to Improve Conidial Yield and Stress Resistance of Trichoderma guizhouense through Optimizing Illumination Conditions

Meng

Guo

et al. 2022

JoF

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Light is perceived by photoreceptors in fungi and further integrated into the stress-activated MAPK HOG pathway, and thereby potentially activates the expression of genes for stress responses. This indicates that the precise control of light conditions can likely improve the conidial yield and stress resistance to guarantee the low cost and long shelf life of Trichoderma-based biocontrol agents and biofertilizers. In this study, effects of wavelengths and intensities of light on conidial yield and stress tolerance to osmotic, oxidative and pH stresses in Trichoderma guizhouense were investigated. We found that 2 μmol photons/(m2 × s) of blue light increased the conidial yield more than 1000 folds as compared to dark condition and simultaneously enhanced conidial stress resistance. The enhanced conidial stress resistance is probably due to the upregulated stress-related genes in blue light, which is under the control of the blue light receptor BLR1 and the MAP kinase HOG1.

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Conidiation under illumination enhances conidial tolerance of insect-pathogenic fungi to environmental stresses

Cited by 27 publications

References 74 publications

Entomopathogenicity ofSimplicillium lanosoniveumCG888 on Mortality ofCallosobruchus maculatusF. in vitro

Entomopathogenicity ofSimplicillium lanosoniveumCG888 on Mortality ofCallosobruchus maculatusF. in vitro

Light in the box—photobiological examination chamber with light trap ventilation system for studying fungal surface cultures illustrated with Metarhizium brunneum and Beauveria brongniartii

A Simple and Low-Cost Strategy to Improve Conidial Yield and Stress Resistance of Trichoderma guizhouense through Optimizing Illumination Conditions

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