Conjugal Transfer of Transposon Tn916 from Enterococcus faecalis to Bacillus licheniformis

Herzog-Velikonja, Blagajana; Podlesek, Zdravko; Grabnar, M.

doi:10.1006/plas.1994.1021

Cited by 2 publications

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“…A single PCR product of the same size with both of the primer pairs used (DI-DII and tet1-tet2) was obtained for 11 B. longum, 5 B. bifidum , 3 L. lactis, 1 B. animalis and 1 L. johnsonii strains ( table 3 ). More particularly, the sequences from the amplicons of L. lactis proved to be 100% identical to the tet (M) gene present in the Tn 916 transposon of Enterococcus faecalis [Herzog-Velikonja et al, 1994]. A section of the nucleotide sequence of the amplicon obtained from L. johnsonii showed 99% homology with tet (W) genes, while another region matched the sequence of tet (O) genes, suggesting the determinant for this strain may be mosaic (data not shown).…”

Section: Screening For Resistance Genes By Pcr and Sequencingmentioning

confidence: 92%

Molecular Characterization of Intrinsic and Acquired Antibiotic Resistance in Lactic Acid Bacteria and Bifidobacteria

Ammor

Flórez

Hoek³

et al. 2007

Microb Physiol

146

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The minimum inhibitory concentrations (MICs) of 6 different antibiotics (chloramphenicol, clindamycin, erythromycin, streptomycin, tetracycline and vancomycin) were determined for 143 strains of lactic acid bacteria and bifidobacteria using the Etest. Different MICs were found for different species and strains. Based on the distribution of these MIC values, most of the strains were either susceptible or intrinsically resistant to these antibiotics. However, the MIC range of some of these antibiotics showed a bimodal distribution, which suggested that some of the tested strains possess acquired antibiotic resistance. Screening for resistance genes was performed by PCR using specific primers, or using a DNA microarray with around 300 nucleotide probes representing 7 classes of antibiotic resistance genes. The genes identified encoded resistance to tetracycline [tet(M), tet(W), tet(O) and tet(O/W)], erythromycin and clindamycin [erm(B)] and streptomycin [aph(E) and sat(3)]. Internal portions of some of these determinants were sequenced and found to be identical to genes described in other bacteria. All resistance determinants were located on the bacterial chromosome, except for tet(M), which was identified on plasmids in Lactococcus lactis. The contribution of intrinsic multidrug transporters to the antibiotic resistance was investigated by cloning and measuring the expression of Bifidobacterium breve genes in L. lactis.

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Section: Screening For Resistance Genes By Pcr and Sequencingmentioning

confidence: 92%