Conjugated linoleic acid and cardiac health: Oxidative stress and energetic metabolism in standard and sucrose-rich diets

Diniz, Yeda S.; Santos, Priscila P.; Assalin, Heloísa Balan; Souza, Gisele Aparecida; Rocha, Katiucha K. H. R.; Ebaid, Geovana Xavier; Seiva, Fábio Rodrigues Ferreira; Amauchi, Juliana Fujihara; Filho, José Luiz V. B. Novelli; Novelli, Ethel L. B.

doi:10.1016/j.ejphar.2007.11.008

Cited by 23 publications

(16 citation statements)

References 34 publications

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“…In fructose‐ and sucrose‐fed rats, the activities of a range of anti‐oxidant enzymes in ventricular tissue (i.e. SOD, catalase, glutathione peroxidase and glutathione reductase) are significantly reduced and increased lipid peroxidation is observed 43,74,83 . Delbosc et al.…”

Section: Role For Oxidative Stress In Insulin‐resistant Cardiomyopathymentioning

confidence: 99%

Reactive oxygen species and insulin‐resistant cardiomyopathy

Mellor

Ritchie

Delbridge

2010

Clin Exp Pharma Physio

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1. The prevalence of insulin resistance has increased markedly in the past decade and is known to be associated with cardiovascular risk. Evidence of an insulin-resistant cardiomyopathy, independent of pressure or volume loading influences, is now emerging. 2. Cardiac oxidative stress is often observed coincident with insulin resistance and there is accumulating evidence that reactive oxygen species (ROS) mediate deleterious effects in the insulin-resistant heart. It is established that ROS modification of signalling proteins can adversely modulate cellular processes, leading to cardiac growth remodelling and dysfunction. The mechanisms of ROS-induced damage in insulin-resistant cardiomyopathy are yet to be fully elucidated. 3. A number of different animal models have been used to study cardiac insulin resistance, including high-sugar dietary interventions, genetically modified diabetic mice and streptozotocin-induced diabetes. Mechanistic studies manipulating cardiac anti-oxidant levels, either endogenously or exogenously, in these models have demonstrated a role for ROS in the cardiac manifestations associated with insulin resistance. 4. The present review summarizes the cardiac-specific characteristics of insulin resistance, the features of cardiac metabolism relevant to ROS generation and ROS-mediated cardiomyocyte damage pathways. In vivo studies in which a combination of genetic and environmental variables have been manipulated are considered. These studies provide particular insights into the induction and suppression of insulin-resistant cardiomyopathy.

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Section: Role For Oxidative Stress In Insulin‐resistant Cardiomyopathymentioning

confidence: 99%

Reactive oxygen species and insulin‐resistant cardiomyopathy

Mellor

Ritchie

Delbridge

2010

Clin Exp Pharma Physio

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“…ii) Male c57bl/6 mice fed high-fat, high-sucrose diet for 8 months showed increased rate of H 2 O 2 production, decreased glutathione activity and increased oxidative-induced posttranslational modifications [60]. iii) Many reports that refer to increased oxidative stress in the diabetic cardiomyopathy were performed with animal models where high-sugar diets were used [4, 61–64]. Furthermore, the increased insulin levels associated with insulin resistance may affect cardiac function: insulin has been shown to inhibit β-adrenergic stimulation and hence impair β-adrenergic-regulated cardiac contractility [65]; application of insulin increased the amplitude of [Ca 2+ ] i transients in wild-type cardiomyocytes, whereas it broadened the transients and triggered extra [Ca 2+ ] i transients in ob / ob cardiomyocytes [18].…”

Section: Discussionmentioning

confidence: 99%

The Role of Reactive Oxygen Species in β-Adrenergic Signaling in Cardiomyocytes from Mice with the Metabolic Syndrome

et al. 2016

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The metabolic syndrome is associated with prolonged stress and hyperactivity of the sympathetic nervous system and afflicted subjects are prone to develop cardiovascular disease. Under normal conditions, the cardiomyocyte response to acute β-adrenergic stimulation partly depends on increased production of reactive oxygen species (ROS). Here we investigated the interplay between beta-adrenergic signaling, ROS and cardiac contractility using freshly isolated cardiomyocytes and whole hearts from two mouse models with the metabolic syndrome (high-fat diet and ob/ob mice). We hypothesized that cardiomyocytes of mice with the metabolic syndrome would experience excessive ROS levels that trigger cellular dysfunctions. Fluorescent dyes and confocal microscopy were used to assess mitochondrial ROS production, cellular Ca2+ handling and contractile function in freshly isolated adult cardiomyocytes. Immunofluorescence, western blot and enzyme assay were used to study protein biochemistry. Unexpectedly, our results point towards decreased cardiac ROS signaling in a stable, chronic phase of the metabolic syndrome because: β-adrenergic-induced increases in the amplitude of intracellular Ca2+ signals were insensitive to antioxidant treatment; mitochondrial ROS production showed decreased basal rate and smaller response to β-adrenergic stimulation. Moreover, control hearts and hearts with the metabolic syndrome showed similar basal levels of ROS-mediated protein modification, but only control hearts showed increases after β-adrenergic stimulation. In conclusion, in contrast to the situation in control hearts, the cardiomyocyte response to acute β-adrenergic stimulation does not involve increased mitochondrial ROS production in a stable, chronic phase of the metabolic syndrome. This can be seen as a beneficial adaptation to prevent excessive ROS levels.

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“…It should be noted the adult heart normally obtains 50-70% of its ATP from fatty acid β-oxidation and on a physiological condition, FA is considered to account for 60-70% of oxygen consumption for energy production in the heart and high intensity exercise might reduce FA metabolic capacity and increase nonfat oxidation capacity such those happened in aged rats [23,24]. Thus a lower PPAR and LXR mRNA expression in SV-trained heart might be due to a reduction in FA metabolism capacity and the fuel switching from fatty acid oxidation to carbohydrate metabolism [25,26], particularly lactate consumption as a fuel for energy provision in high-intensity and longterm exercise training [27].…”

Section: Discussionmentioning

confidence: 99%

Heart ATP-Binding Cassette protein A1 and G1, Peroxisome Proliferator-Activated Receptor-α and Liver X Receptors Genes Expression in Response to Intensive Treadmill Running and Red Crataegus pentaegyna (Sorkh valik) in Male Rats

Ghanbari–Niaki

Abarghooi

Gholizadeh

2015

Zahedan J Res Med Sci

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Background:The heart has a very high energy demand and to sustain sufficient ATP generation, can use a variety of different carbon substrates as energy sources if available. Objectives:The purpose of the current study was to investigate the effect of a high intensity treadmill running training (8 weeks) with or without aqueous extraction of Crataegus pentaegyna (Sorkh valik) on heart ABCA1, ABCG1, PPARα and LXR genes expression. Materials and Methods:In this experimental study, 20 Wistar male rats (4-6 weeks old, 158.9 ± 9.59 g weight) were used. Animals were randomly assigned into saline-control (SC, n = 5), saline-training (ST, n = 5), Sorkh valik-control (SVC, n = 5) and Sorkh valik-training (SVT, n = 5) groups. Training groups have performed a high intensity running program 34 m/min on a motor-driven treadmill for 8 weeks. Animals were fed orally with Sorkh valik extraction (500 mg/kg) and saline solution for last 6 weeks. Seventy two hours after the last training session, rats were sacrificed, heart were excised, cleaned and immediately frozen in liquid nitrogen and stored at -70°C until RNA extraction. Statistical analysis was performed using a two way ANOVA, and significance was accepted at P < 0.05. Results: Changes in ABCA1 and ABCG1 gene expression were not significant and a non-significant increase in PPAR-α and LXR genes expression were found in the trained rats and Sorkh valik-control group than in the control group. Conclusions: The findings indicate that training and Sorkh valik extraction solely were able to increase non-significantly all selected genes expression in rat heart. It seems that exercise with Sorkh valik supplementation trend to decrease mentioned genes expression.

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Conjugated linoleic acid and cardiac health: Oxidative stress and energetic metabolism in standard and sucrose-rich diets

Cited by 23 publications

References 34 publications

Reactive oxygen species and insulin‐resistant cardiomyopathy

Reactive oxygen species and insulin‐resistant cardiomyopathy

The Role of Reactive Oxygen Species in β-Adrenergic Signaling in Cardiomyocytes from Mice with the Metabolic Syndrome

Heart ATP-Binding Cassette protein A1 and G1, Peroxisome Proliferator-Activated Receptor-α and Liver X Receptors Genes Expression in Response to Intensive Treadmill Running and Red Crataegus pentaegyna (Sorkh valik) in Male Rats

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