Conjugated linoleic acid (CLA) content and fatty acids composition of muscle in rats fed isomers of CLA and selenium

Abstract

The aim of the study was to determine the influence of conjugated isomers of linoleic acid (CLA) and/or selenium (Se) on the body mass gain of rats, feed intake, and the levels of CLA isomers and some other fatty acids in the femoral muscle. Rat diets enriched in Se, the trans-10,cis-12 isomer and mixtures of the CLA isomers (regardless Se supplementation) tended to decrease body mass gain compared with control rats, while simultaneously supplementation with Se and the trans-10,cis-12 isomer resulted in the highest increase. A mixture of CLA isomers and/or Se supplementation, and the trans-10,cis-12 isomer decreased feed intake, whereas the cis-9,trans-11 isomer, regardless of Se supplementation, only slightly influence of feed intake. Enriching diets with CLA isomers increased their level in muscles as well as the level of non-CLA isomers containing conjugated double bonds (CD). In rats receiving simultaneously Se and mixtures of the CLA isomers or individual CLA isomers the CLA isomers and CD levels in muscles were higher compared with rats fed only the CLA isomers. Rats fed individual isomers or their mixture showed minute changes in C8:0 and C10:0 concentrations in muscles, while the C12:0, C14:0, C16:0 and C18:0 contents were generally lower. A small increase in C10:0 and C16:0 content was found in rats fed the trans-10,cis-12 isomer. Supplementing Se to diets enriched in CLA isomers usually resulted in an increase in the saturated fatty acid content in muscles in comparison with rats fed only CLA isomers. The linoleic acid content in muscles was enhanced by the Se supplemented diets enriched in the trans-10,cis-12 isomer or in the 2% CLA isomer mixture, although 1% mixture of the CLA isomers or the cis-9,trans-11 isomer dosed simultaneously with Se also increased the level of linoleic acid, albeit insignificantly. The Se supplemented diet enriched 184 CLA IN MUSCLES OF RATS FED CLA ISOMERS AND SE in the trans-10,cis-12 isomer or 2% mixture of the CLA isomers produced the highest increase of γ-linolenic acid, the total fatty acid and polyunsaturated fatty acid content in rat muscle.

Dietary lycopenes, selenium compounds and fish oil affect the profile of fatty acids and oxidative stress in chicken breast muscle

“…These observations are consistent with our recent studies in which a diet enriched in SeVI or SeY also stimulated the accumulation of c9t11CLA as well as t10c12CLA, ccCLA, and ttCLA in the femoral muscle of rats (Czauderna et al, 2004(Czauderna et al, , 2011a. Thus, considering the current findings and our previous studies, we conclude that dietary Se VI or SeY decreased the metabolism of CLA isomers in chickens and rats.…”

“…Considering the above, it is reasonable to argue that dietary Se VI reduced the yield of LA metabolism in pullets and, in particular, in cockerels. Thus, the current results and our recent studies on rats (Czauderna et al, 2004) also reinforce the finding that dietary Se VI decreased the capacity of enzymes (like elongase and desaturases) responsible for the anabolism of LA in muscles of both chickens and rats. Consequently, Se VI added to the ration with Lyc most efficiently increased the concentration of n-6 PUFA (n-6; Table 4), as well as the value of the n-6 PUFA/n-3 PUFA ratio (n-6/n-3) in breast muscles of pullets and, especially, cockerels (Table 5).…”

“…Interestingly, the ration containing Lyc and Se VI most efficiently increased the value of the PUFA/SFA ratio in muscles of pullets and, especially, in cockerels. Our earlier data on this subject (Czauderna et al, 2004) also reinforce the current finding that dietary Se VI elevated the value of the PUFA/SFA ratio in muscles of chickens in comparison with the other groups (Table 5).…”

The influence of dietary conjugated linoleic acid isomers and Se on the fatty acid profile in rat blood plasma and selected tissues

“…Ten groups of 7-8 female rats, 8 weeks of age and an initial body weight of about 200 g (Table 1) were housed individually as described previously (Czauderna et al, 2004b). Rats (Wistar, Ifz:BOA) were fed ad libitum the Labofeed diet (Control) or diets enriched in CLA isomer(s) and/or 2 ppm Se (as Na 2 SeO 4 ) ( Table 1).…”

“…The resulting solution was mixed again and reacted for 30 min at 40°C (Czauderna et al, 2004b). The processed samples were then injected onto C 18 Nova Pak columns (Czauderna et al, 2004b).…”