Conjugated linoleic acid (CLA) induces apoptosis and inhibits coupling of heregulin (HRG) signaling to phosphatidylinositol 3-kinase (PI3K) and Akt via ErbB3 phosphorylation in human colon cancer Ht-29 cells

Park, Jung H.; Cho, Han J.; Kim, Woo Kyun; Kim, Eun J.; Tyner, Angela L.

doi:10.1016/s0016-5085(03)81410-5

Cited by 2 publications

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“…Li et al indicated that in Caco2 cells, LA up-regulated both mRNA and protein level of ApoA-IV which was produced dominantly in intestine and functioned anti-in ammation. [9,10] And in Ht-29 cells, LA isomer inhibits cell proliferation and stimulates apoptosis [11]. Moreover, In human, duodenal ulcer was associated with de ciency of dietary LA intake [12].…”

Section: Introductionmentioning

confidence: 99%

Maternal Dietary Linoleic Acid Altered Intestinal Barrier Function in Domestic Pigeons (Columba Livia)

Zou

Dong

2020

Preprint

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BackgroundLinoleic acid (LA) is predominantly essential for poultry. Deficiency of LA in poultry were manifested in various aspects such as retarded growth and reduced resistance to disease. The effects of LA on intestinal health in vitro and in mammals has been studied, whereas research related to the effects of LA on intestine health in poultry was scanty. Intestinal health and immune function play an important role in pigeon squab growth. Considering squabs are fed by their parents, the purpose of this study was to explore the effects of maternal dietary LA on intestinal barrier function in squabs by determining intestinal morphology, gene expression of tight junction protein, immune cytokines, and microbial flora.ResultsA completely randomized design with a control group, 1% dietary LA supplementation group, 2% dietary LA supplementation group, and 4% dietary LA supplementation group was used. Six squabs from each treatment were randomly sampled at 21 posthatch. Results indicated that LA supplementation improved intestinal morphology as reflected by increased villus height, villus area and the ratio of villus to crypt, and the promotion at dosage of 1% was most significant. Besides, 1% LA supplementation elevated distribution density of goblet cell in intestine, and strengthened tight junction between enterocytes by up-regulating claudin3 and occludin gene expression, but down-regulating claudin2 gene expression. Moreover, 1% LA supplementation reduced secretion of pro-inflammation cytokines and increased anti-inflammation cytokines partly. The diversity index Chao1 of intestinal microbiota in 1% LA supplementation group was higher than other groups. And Butyrivibrio as beneficial bacteria was the biomarker of LA1%. However, excessive (4%) LA supplementation led to adverse impact on intestinal immunity and microbiota.ConclusionsMaternal dietary LA in three levels all could improve intestinal morphology in squabs. Therein, appropriate dosage (1%) supplementation might enhance mucosal protection and epithelium barrieer in squabs, and furthermore consolidated intestine immunity and luminal microbial environment. However, excessive (4%) LA supplementation might lead to adverse impact on immunity and microbiota. Maternal dietary LA might alter intestinal barrier function in pigeon squabs in a dose-dependent manner.

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Section: Introductionmentioning

confidence: 99%

Maternal Dietary Linoleic Acid Altered Intestinal Barrier Function in Domestic Pigeons (Columba Livia)

Zou

Dong

2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Studies in vivo and in vitro have focused on the effects of LA on intestinal health and immune function over the years (9)(10)(11)(12)(13) . Li et al (9) indicated that in Caco2 cells, LA up-regulated both the mRNA and protein levels of ApoA-IV which is produced predominantly in the intestine and functions in anti-inflammation (14) .…”

mentioning

confidence: 99%

“…Li et al (9) indicated that in Caco2 cells, LA up-regulated both the mRNA and protein levels of ApoA-IV which is produced predominantly in the intestine and functions in anti-inflammation (14) . In Ht-29 cells, the LA isomer inhibits cell proliferation and stimulates apoptosis (10) . Moreover, in humans, duodenal ulcers were associated with a deficiency of dietary LA intake (11) .…”

mentioning

confidence: 99%

Maternal dietary linoleic acid altered intestinal barrier function in domestic pigeons (Columba livia)

Wen

Wang

et al. 2020

Br J Nutr

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Linoleic acid (LA) is predominantly essential for poultry. Poultry lacking LA show retarded growth and reduced disease resistance. Intestinal barrier function plays an important role in pigeon squab growth, whereas research on the effects of LA on intestinal health in altrices is scant. Considering that squabs are fed by their parents, the study aimed to explore the effects of maternal dietary LA on intestinal morphology, tight junction proteins, immune cytokines and microbial flora in squabs. A completely randomised design with a control group, 1 % LA supplementation group, 2 % LA supplementation group and 4 % LA supplementation group was used. Six squabs from each treatment were randomly sampled at 21 d post-hatching. The results indicated that LA supplementation improved intestinal morphology, as reflected by increased villus height, villus area and the ratio of villi to crypts. Also, 1 % LA supplementation elevated the density of goblet cells in the intestine and strengthened tight junctions by up-regulating claudin-3 and occludin gene expression but down-regulating claudin-2 gene expression. Moreover, 1 % LA supplementation reduced the secretion of proinflammatory cytokines and partly increased anti-inflammatory cytokines. The intestinal microbial diversity in the 1 % LA supplementation group was higher than that in the other groups. As beneficial bacteria, Butyrivibrio was the biomarker of 1 % LA supplementation. However, excessive (4 %) LA supplementation led to adverse impacts on intestinal immunity and microbiota. In conclusion, maternal dietary LA might alter intestinal barrier function in pigeon squabs in a dose-dependent manner. Supplementation with 1 % LA was suggested in parental pigeons.

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Conjugated linoleic acid (CLA) induces apoptosis and inhibits coupling of heregulin (HRG) signaling to phosphatidylinositol 3-kinase (PI3K) and Akt via ErbB3 phosphorylation in human colon cancer Ht-29 cells

Cited by 2 publications

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Maternal Dietary Linoleic Acid Altered Intestinal Barrier Function in Domestic Pigeons (Columba Livia)

Maternal Dietary Linoleic Acid Altered Intestinal Barrier Function in Domestic Pigeons (Columba Livia)

Maternal dietary linoleic acid altered intestinal barrier function in domestic pigeons (Columba livia)

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