Conjugating folic acid to gold nanoparticles through glutathione for targeting and detecting cancer cells

Zhang, Zhaowu; Jia, Junmei; Lai, Youqun; Ma, Yanyan; Weng, Jian; Sun, Liping

doi:10.1016/j.bmc.2010.06.045

Cited by 177 publications

(124 citation statements)

References 45 publications

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“…GSH carries two deprotonated anionic carboxyl groups and one protonated cationic amine group. Thus cationic amine group nullified charge of one carboxyl group of GSH and in comparison to citrate-AuNPs, the AuNPs-GSH carries less negative charge [23] . With GCV loaded AuNPs-GSH, higher value of -14.61 mV was observed.…”

Section: Resultsmentioning

confidence: 97%

“…2 The surface of citrate-AuNPs was further modified by GSH. GSH consists of thiol groups due to presence of cysteine moiety [23] . Citrate ions were replaced by GSH molecules through strong Au-S bond.…”

Section: Resultsmentioning

confidence: 99%

“…Surface modification was done by GSH. Briefly 300 µl of 0.05 M solution of GSH was added to 50 ml of citrate-AuNPs and kept for next 12 h under stirring [23] The resulted suspension was further dialysed (12 kD) for next 12 h to remove excess GSH molecules.…”

Section: Surface Modification Of Synthesized Citrate-reduced Gold Nanmentioning

confidence: 99%

“…Zhang et al [23] also conjugated folic acid with glutathione (GSH) modified AuNPs. GSH is a natural tripeptide containing thiol, amino and carboxylic groups.…”

mentioning

confidence: 99%

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Preparation and Characterization of Ganciclovir Loaded Glutathione Modified Gold Nanoparticles

Kiroula¹,

Negi²

2016

pharmaceutical-sciences

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Kiroula, et al.: Glutathione-modified Gold Nanoparticles of GanciclovirThis article reports that ganciclovir-loaded gold nanoparticles were developed via glutathione surface modification. Citrate-reduced gold nanoparticles were prepared first from chloroauric acid. Further the surface of gold nanoparticles was modified with glutathione. Then ganciclovir was loaded onto the glutathione-modified gold nanoparticles through the reaction between carboxyl group of glutathione and amino group of ganciclovir. Gold nanoparticles were characterized by photon correlation spectroscopy, transmission electron microscopy and infrared spectroscopy. Drug loading of 87.27±2.52% was observed for ganciclovir-loaded gold nanoparticles. The particle size distribution of gold nanoparticles ranged from 26.3 to 31 nm. Thus ganciclovir-loaded gold nanoparticles were successfully prepared, which might further improve the oral bioavailability of ganciclovir.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Surface Modification Of Synthesized Citrate-reduced Gold Nanmentioning

confidence: 99%

“…Zhang et al [23] also conjugated folic acid with glutathione (GSH) modified AuNPs. GSH is a natural tripeptide containing thiol, amino and carboxylic groups.…”

mentioning

confidence: 99%

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Preparation and Characterization of Ganciclovir Loaded Glutathione Modified Gold Nanoparticles

Kiroula¹,

Negi²

2016

pharmaceutical-sciences

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“…The bands at 1,380 cm -1 (-NH) came from bending vibration peak of amino acids. 31,32 All the abovementioned peaks confirmed that Gd-CDs were successfully attached to the surface of AFn through the amidation process.…”

mentioning

confidence: 72%

Construction of magnetic-carbon-quantum-dots-probe-labeled apoferritin nanocages for bioimaging and targeted therapy

Yao¹,

Su²,

Zeng³

et al. 2016

IJN

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Carbon dots (CDs) are one of the most highlighted carbon-based materials for biological applications, such as optical imaging nanoprobes, which are used for labeling cells in cancer treatment mainly due to their biocompatibility and unique optical properties. In this study, gadolinium (Gd)-complex-containing CDs were obtained through a one-step microwave method to develop multimodal nanoprobes integrating the advantages of optical and magnetic imaging. The obtained Gd-CDs exhibited highly fluorescent properties with excellent water solubility and biological compatibility. Natural apoferritin (AFn) nanocages, an excellent drug delivery carrier, are hollow in structure, with their pH-dependent, unfolding–refolding process at pH 2.0 and 7.4. The chemotherapeutic drug doxorubicin (DOX) can be highly effective and encapsulated into AFn cavity. A widely used tumor-targeting molecule, folic acid (FA), functionalized the surface of AFn to obtain an active tumor targeting effect on MCF-7 cells and malignant tumors in mice models. In this study, an AFn nanocarrier encapsulating high concentration of DOX labeled with magnetic and fluorescent Gd-CDs probe was developed. Gd-CDs exhibited a unique green photoluminescence and almost no toxicity compared with free GdCl 3 . Furthermore, Gd-doped CDs significantly increased the circulation time and decreased the toxicity of Gd 3+ in in vitro and in vivo magnetic resonance imaging, which demonstrated that the AFn nanocages labeled with Gd-CD compounds could serve as an excellent T 1 contrast agent for magnetic resonance imaging. The self-assembling multifunctional Gd-CDs/AFn (DOX)/FA nanoparticles have a great potential for cancer theranostic applications.

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Highly Luminescent Folate‐Functionalized Au₂₂ Nanoclusters for Bioimaging

Pyo

Yoon

et al. 2017

Adv Healthcare Materials

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Gold nanoclusters are emerging as new materials for biomedical applications because of promises offered by their ultrasmall size and excellent biocompatibility. Here, the synthesis and optical and biological characterizations of a highly luminescent folate-functionalized Au cluster (Au -FA) are reported. The Au -FA clusters are synthesized by functionalizing the surface of Au (SG) clusters, where SG is glutathione, with benzyl chloroformate and folate. The functionalized clusters are highly water-soluble and exhibit remarkably bright luminescence with a quantum yield of 42%, significantly higher than any other water-soluble gold clusters protected with thiolate ligands. The folate groups conjugated to the gold cluster give rise to additional luminescence enhancement by energy transfer sensitization. The brightness of Au -FA is found to be 4.77 mM cm , nearly 8-fold brighter than that of Au (SG) . Further biological characterizations have revealed that the Au -FA clusters are well-suited for bioimaging. The Au -FA clusters exhibit excellent photostability and low toxicity; nearly 80% cell viability at 1000 ppm of the cluster. Additionally, the Au -FA clusters show target specificity to folate-receptor positive cells. Finally, the time-course in vivo luminescence images of intravenous-injected mice show that the Au -FA clusters are renal-clearable, leaving only 8% of them remained in the body after 24 h post-injection.

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Conjugating folic acid to gold nanoparticles through glutathione for targeting and detecting cancer cells

Cited by 177 publications

References 45 publications

Preparation and Characterization of Ganciclovir Loaded Glutathione Modified Gold Nanoparticles

Preparation and Characterization of Ganciclovir Loaded Glutathione Modified Gold Nanoparticles

Construction of magnetic-carbon-quantum-dots-probe-labeled apoferritin nanocages for bioimaging and targeted therapy

Highly Luminescent Folate‐Functionalized Au₂₂ Nanoclusters for Bioimaging

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Conjugating folic acid to gold nanoparticles through glutathione for targeting and detecting cancer cells

Cited by 177 publications

References 45 publications

Preparation and Characterization of Ganciclovir Loaded Glutathione Modified Gold Nanoparticles

Preparation and Characterization of Ganciclovir Loaded Glutathione Modified Gold Nanoparticles

Construction of magnetic-carbon-quantum-dots-probe-labeled apoferritin nanocages for bioimaging and targeted therapy

Highly Luminescent Folate‐Functionalized Au22 Nanoclusters for Bioimaging

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Highly Luminescent Folate‐Functionalized Au₂₂ Nanoclusters for Bioimaging