Conjunctival Sac Microbiome in Infectious Conjunctivitis

Mohamed, Yasser Helmy; Uematsu, Masafumi; Morinaga, Yoshitomo; Nguyen, Hien-Anh Thi; Toizumi, Michiko; Sasaki, Daisuke; Yanagihara, Katsunori; Dang, Duc-Anh; Kitaoka, Takashi; Yoshida, Lay-Myint

doi:10.3390/microorganisms9102095

Cited by 5 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the bovine ocular microbial community is a potential new target for manipulation, due to diseases such as IBK ( Brown et al, 1998 ), which is a significant health issue affecting the beef cattle industry. Human studies suggest that the ocular surface microbiota is associated with ocular health ( Matysiak et al, 2021 ; Mohamed et al, 2021 ), and that a healthy ocular microbiota may provide resistance against colonization from infectious agents ( Teweldemedhin et al, 2017 ; Chiang and Chern, 2022 ). While the association of the ocular microbiota with IBK is yet to be determined, a recent study revealed a relative greater abundance of Moraxella spp.…”

Section: Discussionmentioning

confidence: 99%

Whole-body microbiota of newborn calves and their response to prenatal vitamin and mineral supplementation

et al. 2023

View full text Add to dashboard Cite

Early life microbial colonization and factors affecting colonization patterns are gaining interest due to recent developments suggesting that early life microbiome may play a role in Developmental Origins of Health and Disease. In cattle, limited information exists on the early microbial colonization of anatomical sites involved in bovine health beyond the gastrointestinal tract. Here, we investigated 1) the initial microbial colonization of seven different anatomical locations in newborn calves and 2) whether these early life microbial communities and 3) serum cytokine profiles are influenced by prenatal vitamin and mineral (VTM) supplementation. Samples were collected from the hoof, liver, lung, nasal cavity, eye, rumen (tissue and fluid), and vagina of beef calves that were born from dams that either received or did not receive VTM supplementation throughout gestation (n = 7/group). Calves were separated from dams immediately after birth and fed commercial colostrum and milk replacer until euthanasia at 30 h post-initial colostrum feeding. The microbiota of all samples was assessed using 16S rRNA gene sequencing and qPCR. Calf serum was subjected to multiplex quantification of 15 bovine cytokines and chemokines. Our results indicated that the hoof, eye, liver, lung, nasal cavity, and vagina of newborn calves were colonized by site-specific microbiota, whose community structure differed from the ruminal-associated communities (0.64 ≥ R2 ≥ 0.12, p ≤ 0.003). The ruminal fluid microbial community was the only one that differed by treatment (p < 0.01). However, differences (p < 0.05) by treatment were detected in microbial richness (vagina); diversity (ruminal tissue, fluid, and eye); composition at the phylum and genus level (ruminal tissue, fluid, and vagina); and in total bacterial abundance (eye and vagina). From serum cytokines evaluated, concentration of chemokine IP-10 was greater (p = 0.02) in VTM calves compared to control calves. Overall, our results suggest that upon birth, the whole-body of newborn calves are colonized by relatively rich, diverse, and site-specific bacterial communities. Noticeable differences were observed in ruminal, vaginal, and ocular microbiota of newborn calves in response to prenatal VTM supplementation. These findings can derive future hypotheses regarding the initial microbial colonization of different body sites, and on maternal micronutrient consumption as a factor that may influence early life microbial colonization.

show abstract

Section: Discussionmentioning

confidence: 99%

Whole-body microbiota of newborn calves and their response to prenatal vitamin and mineral supplementation

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Human studies suggest that the ocular surface microbiota is associated with ocular health (80,81), and that a healthy ocular microbiota may provide resistance against colonization from infectious agents (82,83). While the association of the ocular microbiota with IBK is yet to be determined, a recent study revealed a relative greater abundance of Moraxella spp.…”

Section: Discussionmentioning

confidence: 99%

“…However, the bovine ocular microbial community is a potential new target for manipulation, due to diseases such as IBK (79), which is a significant health issue affecting the beef cattle industry. Human studies suggest that the ocular surface microbiota is associated with ocular health (80, 81), and that a healthy ocular microbiota may provide resistance against colonization from infectious agents (82, 83). While the association of the ocular microbiota with IBK is yet to be determined, a recent study revealed a relative greater abundance of Moraxella spp.…”

Section: Discussionmentioning

confidence: 99%

Whole-body Microbiota of Newborn Calves and Their Response to Prenatal Vitamin and Mineral Supplementation

Luecke

Holman

Schmidt

et al. 2023

Preprint

View full text Add to dashboard Cite

Here, we investigated whether initial microbial colonization at seven different anatomical locations in newborn calves and their blood cytokines are influenced by prenatal vitamin and mineral (VTM) supplementation. Samples were collected from the hoof, liver, lung, nasal cavity, eye, rumen (tissue and fluid), and vagina of beef calves that were born from dams that received diets with or without VTM supplementation throughout gestation (n=7/group). Calves were separated from their dams immediately after birth and fed colostrum and milk replacer until euthanasia at 30 h post-initial colostrum feeding. The microbiota of all samples was assessed using 16S rRNA gene sequencing and qPCR. 15 cytokines and chemokines were quantified in their serum. The hoof, ocular, liver, respiratory, and reproductive sites of newborn calves were colonized by site-specific microbiota that differed from that of the rumen (0.64 ≥ R2 ≥ 0.12, P ≤ 0.003). Only the ruminal fluid microbiota was differed by on prenatal VTM supplementation (P<0.01 Differences (P<0.05) were detected in microbial richness (vagina), diversity (ruminal tissue and fluid, eye), composition at the phylum and genus level (ruminal tissue and fluid, and vagina), and total bacterial abundance (ocular and vagina) between VTM and control calves. The cytokine IP-10 was higher (P=0.02) in VTM calves. Overall, our results suggest that despite immediate separation from the dam upon birth, whole-body of 32-h old calves are colonized by relatively rich, diverse and site-specific bacterial communities, and that initial microbial colonization of the rumen, vagina and oculus seem to be influenced by the prenatal VTM supplementation.

show abstract

Microbiological spectrum and antibiotic susceptibility patterns of non-viral conjunctivitis over 16 years at a tertiary eye care center in Southern India

2023

View full text Add to dashboard Cite

Conjunctival Sac Microbiome in Infectious Conjunctivitis

Cited by 5 publications

References 29 publications

Whole-body microbiota of newborn calves and their response to prenatal vitamin and mineral supplementation

Whole-body microbiota of newborn calves and their response to prenatal vitamin and mineral supplementation

Whole-body Microbiota of Newborn Calves and Their Response to Prenatal Vitamin and Mineral Supplementation

Microbiological spectrum and antibiotic susceptibility patterns of non-viral conjunctivitis over 16 years at a tertiary eye care center in Southern India

Contact Info

Product

Resources

About