2017
DOI: 10.1111/nph.14662
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Connecting active to passive fluorescence with photosynthesis: a method for evaluating remote sensing measurements of Chl fluorescence

Abstract: Recent advances in the retrieval of Chl fluorescence from space using passive methods (solar-induced Chl fluorescence, SIF) promise improved mapping of plant photosynthesis globally. However, unresolved issues related to the spatial, spectral, and temporal dynamics of vegetation fluorescence complicate our ability to interpret SIF measurements. We developed an instrument to measure leaf-level gas exchange simultaneously with pulse-amplitude modulation (PAM) and spectrally resolved fluorescence over the same fi… Show more

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Cited by 105 publications
(107 citation statements)
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References 91 publications
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“…Clearly, more in-depth processbased studies are needed to understand the nature of the SIF-GPP relationship. A particular emphasis should be placed on the covariation between F CO2 and F F at different spatiotemporal scales, which will be key to using SIF as a shortcut to estimating GPP at large scales (40).…”
Section: High-resolution Studies Of Vegetation Functional Gradients Wmentioning
confidence: 99%
“…Clearly, more in-depth processbased studies are needed to understand the nature of the SIF-GPP relationship. A particular emphasis should be placed on the covariation between F CO2 and F F at different spatiotemporal scales, which will be key to using SIF as a shortcut to estimating GPP at large scales (40).…”
Section: High-resolution Studies Of Vegetation Functional Gradients Wmentioning
confidence: 99%
“…Here, we define leaf‐level ChlF as F t,λ , and canopy‐level ChlF as SIF λ , both measuring the absolute radiant energy flux of ChlF from remote sensing platforms (e.g., W·m −2 ·nm −1 ·sr −1 ; equation ): Fnormalt,normalλ,SIFλ=APAR*βλ*[]()fPSII*ΦPSII*Snormalλ,PSII+()()1fPSII*ΦPSI*Snormalλ,PSI where APAR is absorbed photosynthetically active radiation (μmol·m −2 ·s −1 ), β λ is the spectrally dependent leaf or canopy escape probability, f PSII is the fraction of photons absorbed by PSII photosystems, Φ is fluorescence yield of PSII and PSI (quanta emitted/quanta absorbed), and S is the spectral shape of PSII and PSI (J·μmol −1 ·nm −1 ·sr −1 ). In contrast, steady‐state fluorescence from PAM (F t ), typically recorded in millivotls, can be expressed as Ft=APARML*β650850*[]()fPSII*ΦPSII*S650850_PSII+()()1fPSII*ΦPSI*S650850_PSI*c where APAR ML is the absorbed radiant energy from a fixed output ML and the wavelength dependency of β and S is simply measured as the integral of emitted fluorescence somewhere in the 650‐ to 850‐nm range (depending on the manufacturer; Magney, Frankenberg, et al, ), while c is a factor that accounts for the preset sensitivity of the photodiode (mV/[W·m −2 ·nm −1 ·sr −1 ]). The spectral shape ( S ) of ChlF has two peaks centered in the red (~685nm) and far‐red (~740 nm) spectrum, with nonequal contributions from PSI and PSII.…”
Section: Introductionmentioning
confidence: 99%
“…The method, which consists on painting the sample black and measuring the outgoing photon flux density from the integrating sphere before and after painting, was very useful for needles, as it by-passes the need to calculate the gap fraction that otherwise affects the accuracy of transmittance and reflectance spectra (Daughtry et al 1989;Middleton et al 1996;Mesarch et al 1999). Thus, the methodology presented in Study II is expected to facilitate the calculation of leaf PAR absorption, and help avoid the use of constant PAR absorption values in studies dealing with the dynamics of photosynthetic activity (Pieruschka et al 2010;Magney et al 2017).…”
Section: Methods For Assessing Par Absorption In Leavesmentioning
confidence: 99%
“…10), can be estimated by simply multiplying a general value for photochemical yield by the light intensity (Maxwell and Johnson 2000). However, this study suggests that efforts to estimate individual leaf light absorption are worthwhile, especially in studies comparing parameters related to the photochemical activity of various species (Pieruschka et al 2010;Magney et al 2017), or between the leaves of a particular species that differ in age, canopy position or season. This is true because PAR absorption can greatly differ from the commonly assumed 0.84, and thus obscure the actual dynamics of photosynthesis.…”
Section: Par Absorption Dynamics In Leavesmentioning
confidence: 99%
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