Connective tissue elements as diagnostic aids in periodontology

Embery, G.; Waddington, Rachel J.; Hall, Richard L.

doi:10.1034/j.1600-0757.2000.2240109.x

Cited by 47 publications

(50 citation statements)

References 122 publications

(175 reference statements)

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“…Cell Culture: Establishment and serial cultivation of IHGKs has been reviewed recently [16]. To avoid medium-derived induction of keratinocyte differentiation, the IHGKs were maintained in lowcalcium keratinocyte growth medium (basal keratinocyte medium, KGM, with provided supplements, Promocell, Heidelberg, Germany), containing 50 mg ml À1 kanamycin (Roche Diagnostics, Mannheim, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…[15] Contrasting its corresponding epithelium, the CT is poorly populated by cells, while it mainly consists of extracellular-matrix (ECM) molecules including various collagens, FN and a variety of proteoglycans. [16] These CT matrix components which, as a base substance, constitute the cells' microenvironment are synthesized by the cells themselves prior to secretion. Thus, CT fibroblasts produce multiple collagens, among which collagen type-I is the most abundant matrix molecule in the CT.…”

Section: Introductionmentioning

confidence: 99%

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Connective‐Tissue Fibroblasts Established on Micropillar Interfaces are Pivotal for Epithelial‐Tissue Morphogenesis

Müssig

Steinberg

Schulz

et al. 2008

Adv Funct Materials

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Polydimethylsiloxane (PDMS) pillar arrays are applied as a biomechanical microenvironment to establish gingival connective‐tissue fibroblasts (GCTFs) and to further analyze the pivotal role of GCTFs in epithelial‐tissue morphogenesis. GCTFs are known to exert successful adhesion and growth on fibronectin immobilized on pillar heads, over time, concomitant with the increased gene expression of vimentin and collagen type‐I. GCTF‐populated pillar arrays clearly reveal that epithelial‐tissue morphogenesis of immortalized human gingival keratinocytes (IHGKs), co‐cultured for 7 and 14 days, parallels the in vivo phenotype more closely, when compared with GCTF‐free control arrays. This in vivo‐like phenotype is substantiated by higher mRNA levels for keratin 1, involucrin and filaggrin differentiation markers. Furthermore, it is reflected by a tissue‐specific protein orientation of the aforementioned molecules, and also of the cell‐to‐cell contact forming desmoplakin and the basement membrane constituents, laminin‐5, laminin‐1/10, and collagen type‐IV. These experiments suggest that the in vivo‐like phenotype of the IHGK is governed by the GCTFs growing on the micropillar interfaces. Moreover, they form the basis for the optimization or neogeneration of biomaterials by varying predefined microenvironmetal parameters to achieve an in vivo‐like cell growth and differentiation, indispensable for tissue morphogenesis during regeneration.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Connective‐Tissue Fibroblasts Established on Micropillar Interfaces are Pivotal for Epithelial‐Tissue Morphogenesis

Müssig

Steinberg

Schulz

et al. 2008

Adv Funct Materials

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“…Regarding noncollagenous organic matrices of the cementum, immunohistochemistry and biochemistry have identified many distinct molecules, including noncollagenous glycoproteins, proteoglycans, plasma-derived macromolecules, and several growth factors (for reviews, see Embery et al 2000;Waddington and Embery 2001;Bosshardt 2005), and have shown that two noncollagenous glycoproteins, bone sialoprotein (BSP) and osteopontin (OPN), are major constituents of the noncollagenous matrices (McKee et al 1996;Bosshardt et al 1998;Nanci 1999;Bosshardt 2005). The BSP and OPN are closely associated with mineralization, matrix-matrix attachment, and cell-cell/matrix attachment in collagen-based hard tissues (for reviews, see Ganss et al 1999;Sodek et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections

Yamamoto

Domon

Takahashi

et al. 2006

Histochem Cell Biol

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This study was designed to detect tissue non-specific alkaline phosphatase (TNSALP) by Azo-dye staining, calcium by glyoxal bis(2-hydroxyanil) (GBHA) staining, bone sialoprotein (BSP) and osteopontin (OPN) by immunoperoxidase staining in developing rat molars, and also to discuss the mineralization process during acellular cementogenesis. To restrain a reduction in histochemical and immunohistochemical reactions, fresh-frozen undemineralized sections were prepared. Where the epithelial sheath was intact, TNSALP reaction was observed in the dental follicle, but not in the epithelial sheath. With the onset of dentin mineralization, the BSP-and OPN-immunoreactive, initial cementum layer appeared. At this point cementoblasts had shown intense TNSALP reaction and GBHA reactive particles (=calcium-GBHA complex) appeared on the root surface. With further development, the reaction of TNSALP and GBHA became weak on the root surface. Previous studies have shown that the initial cementum is fibril-poor and that matrix vesicles and calciferous spherules appear on the root surface only during the initial cementogenesis. The findings mentioned above suggest that: During the initial cementogenesis cementoblasts release matrix vesicles which result in calciferous spherules, corresponding to the GBHA reactive particles. The calciferous spherules trigger the mineralization of the initial cementum. After principal fiber attachment mineralization advances along collagen fibrils without matrix vesicles.

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“…[22][23][24] It is a complex mixture of substances derived from serum, leukocytes, structural cells of the periodontium, and oral bacteria. 25 In health, GCF represents a transudate of gingival tissue interstitial fluid; however, as gingivitis and periodontitis develop, GCF becomes true inflammatory exudate.…”

Section: Introductionmentioning

confidence: 99%

Effect of rhPDGF-BB Delivery on Mediators of Periodontal Wound Repair

Cooke¹,

Sarment²,

Whitesman³

et al. 2006

Tissue Engineering

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Growth factors such as platelet-derived growth factor (PDGF) exert potent effects on wound healing including the regeneration of tooth-supporting structures. This investigation examined the effect of the local delivery of PDGF-BB when combined with reconstructive periodontal surgery on local wound fluid (WF) levels of PDGF-AB, vascular endothelial growth factor (VEGF), and bone collagen telopeptide (ICTP) in humans with advanced periodontitis. Sixteen patients exhibiting localized periodontal osseous defects were randomized to one of three groups (β-TCP carrier alone, β-TCP + 0.3 mg/mL of recombinant human PDGF-BB [rhPDGF-BB], or β-TCP + 1.0 mg/mL of rhPDGF-BB) and monitored for 6 months. WF was harvested and analyzed for PDGF-AB, VEGF, and ICTP WF levels. Teeth contralateral to the target lesions served as controls. Increased levels of VEGF in the WF was observed for all surgical treatment groups with the 1.0 mg/mL rhPDGF-BB group showing the most pronounced difference at 3 weeks in the AUC analysis versus control (p < 0.0001). PDGF-AB WF levels were increased for the carrier alone group compared to both rhPDGF-BB groups. Low-dose rhPDGF-BB application elicited increases in ICTP at days 3-5 in the wound healing process, suggesting a promotion of bone turnover at early stages of the repair process (p < 0.02). These results demonstrate contrasting inducible expression patterns of PDGF-AB, VEGF, and ICTP during periodontal wound healing in humans.

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Connective tissue elements as diagnostic aids in periodontology

Cited by 47 publications

References 122 publications

Connective‐Tissue Fibroblasts Established on Micropillar Interfaces are Pivotal for Epithelial‐Tissue Morphogenesis

Connective‐Tissue Fibroblasts Established on Micropillar Interfaces are Pivotal for Epithelial‐Tissue Morphogenesis

Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections

Effect of rhPDGF-BB Delivery on Mediators of Periodontal Wound Repair

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