Effect of rhPDGF-BB Delivery on Mediators of Periodontal Wound Repair

Abstract

Growth factors such as platelet-derived growth factor (PDGF) exert potent effects on wound healing including the regeneration of tooth-supporting structures. This investigation examined the effect of the local delivery of PDGF-BB when combined with reconstructive periodontal surgery on local wound fluid (WF) levels of PDGF-AB, vascular endothelial growth factor (VEGF), and bone collagen telopeptide (ICTP) in humans with advanced periodontitis. Sixteen patients exhibiting localized periodontal osseous defects were randomized to one of three groups (β-TCP carrier alone, β-TCP + 0.3 mg/mL of recombinant human PDGF-BB [rhPDGF-BB], or β-TCP + 1.0 mg/mL of rhPDGF-BB) and monitored for 6 months. WF was harvested and analyzed for PDGF-AB, VEGF, and ICTP WF levels. Teeth contralateral to the target lesions served as controls. Increased levels of VEGF in the WF was observed for all surgical treatment groups with the 1.0 mg/mL rhPDGF-BB group showing the most pronounced difference at 3 weeks in the AUC analysis versus control (p < 0.0001). PDGF-AB WF levels were increased for the carrier alone group compared to both rhPDGF-BB groups. Low-dose rhPDGF-BB application elicited increases in ICTP at days 3-5 in the wound healing process, suggesting a promotion of bone turnover at early stages of the repair process (p < 0.02). These results demonstrate contrasting inducible expression patterns of PDGF-AB, VEGF, and ICTP during periodontal wound healing in humans.

Angiogenic Biomarkers and Healing of Living Cellular Constructs

“…Additionally, VEGF together with other angiogenic factors have also been found in the WF of oral wounds (Cooke et al, 2006;Sakai et al, 2006). IL-8 is a chemokine produced by macrophages, epithelial cells, and endothelial cells.…”

Tooth regeneration: a revolution in stomatology and evolution in regenerative medicine

“…[12];  Regeneration of dental pulp (discussed below; c.f. [13]);  Regeneration of dentin based on biological approaches and potentially as biological fillers that may replace current synthetic materials for restorative dentistry [14][15][16];  Regeneration of cementum as a part of periodontium regeneration or for loss of cementum and/or dentin resulting from trauma or orthodontic tooth movement [17][18];  Regeneration of the periodontium including cementum, periodontal ligament and alveolar bone [19][20][21][22];  Regeneration or synthesis of enamel-like structures that may be used as biological substitute for lost enamel [23][24][25]. Since a tooth is a biological organ, it is unavoidable that regeneration of various components of the tooth is highly inter-connected.…”

Advanced Biomatrix Designs for Regenerative Therapy of Periodontal Tissues

“…In fact, the simplest approach is to tether the molecules to the surfaces of biomatrices. This approach is relevant when rapid release is needed, leading to direct contact with the cells and consequent initial cellular activation (Cooke et al, 2006). While surface-tethering of bioactive molecules can be improved via stronger bonds to slow the release rate, a better approach is to incorporate within the biomatrices.…”