Cenotes are habitats
with unique physical, chemical, and biological
features. Unexplored microorganisms from these sinkholes represent
a potential source of bioactive molecules. Thus, a series of cultivable
fungi (
Aspergillus
spp. NCA257, NCA264, and NCA276,
Stachybotrys
sp. NCA252, and
Cladosporium
sp. NCA273) isolated from the cenote Tza Itzá were subjected
to chemical, coculture, and metabolomic analyses. Nineteen compounds
were obtained and tested for their antimicrobial potential against
ESKAPE pathogens,
Mycobacterium tuberculosis
, and
nontuberculous mycobacteria. In particular, phenylspirodrimanes from
Stachybotrys
sp. NCA252 showed significant activity against
MRSA, MSSA, and mycobacterial strains. On the other hand, the absolute
configuration of the new compound 17-deoxy-aspergillin PZ (
1
) isolated from
Aspergillus
sp. NCA276 was established
via single-crystal X-ray crystallography. Also, the chemical analysis
of the cocultures between
Aspergillus
and
Cladosporium
strains revealed the production of metabolites
that were not present or were barely detected in the monocultures.
Finally, molecular networking analysis of the LC-MS-MS/MS data for
each fungus was used as a tool for the annotation of additional compounds,
increasing the chemical knowledge on the corresponding fungal strains.
Overall, this is the first systematic chemical study on fungi isolated
from a sinkhole in Mexico.