Consequences of antisense down-regulation of a lignification-specific peroxidase on leaf and vascular tissue in tobacco lines demonstrating enhanced enzymic saccharification

Kavousi, Bahram; Daudi, Arsalan; Cook, Charis; Joseleau, J.P.; Ruel, K.; Devoto, Alessandra; Bolwell, G. Paul; Blee, Kristopher A.

doi:10.1016/j.phytochem.2010.01.008

Cited by 25 publications

(22 citation statements)

References 62 publications

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“…Notably, we found that lack of AtPRX71 increases biomass production without negative effects on resistance to pathogens, at least in the case of B. cinerea. Because CIII Prxs also negatively regulate saccharification efficiency (Kavousi et al, 2010), crops with both targeted modifications of cell wall composition and reduced levels of selected CIII Prxs may be more efficiently processed without detrimental effects or even a positive impact on biomass production.…”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis thaliana Class III Peroxidase AtPRX71 Negatively Regulates Growth under Physiological Conditions and in Response to Cell Wall Damage.

et al. 2015

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The structure of the cell wall has a major impact on plant growth and development, and alteration of cell wall structural components is often detrimental to biomass production. However, the molecular mechanisms responsible for these negative effects are largely unknown. Arabidopsis (Arabidopsis thaliana) plants with altered pectin composition because of either the expression of the Aspergillus niger polygalacturonase II (AnPGII; 35S:AnPGII plants) or a mutation in the QUASIMODO2 (QUA2) gene that encodes a putative pectin methyltransferase (qua2-1 plants), display severe growth defects. Here, we show that expression of Arabidopsis PEROXIDASE71 (AtPRX71), encoding a class III peroxidase, strongly increases in 35S:AnPGII and qua2-1 plants as well as in response to treatments with the cellulose synthase inhibitor isoxaben, which also impairs cell wall integrity. Analysis of atprx71 loss-of-function mutants and plants overexpressing AtPRX71 indicates that this gene negatively influences Arabidopsis growth at different stages of development, likely limiting cell expansion. The atprx71-1 mutation partially suppresses the dwarf phenotype of qua2-1, suggesting that AtPRX71 contributes to the growth defects observed in plants undergoing cell wall damage. Furthermore, AtPRX71 seems to promote the production of reactive oxygen species in qua2-1 plants as well as plants treated with isoxaben. We propose that AtPRX71 contributes to strengthen cell walls, therefore restricting cell expansion, during normal growth and in response to cell wall damage.

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Section: Discussionmentioning

confidence: 99%

The Arabidopsis thaliana Class III Peroxidase AtPRX71 Negatively Regulates Growth under Physiological Conditions and in Response to Cell Wall Damage.

et al. 2015

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“…LePrxA belongs to group A in which several pathogenic-and wounding-related peroxidases, including LePrx06 (EP5C), NtPrx10a (tpoxN1), StPrx28, and CanPrx01, were also present. LePrx09 and LePrx17 belong to group B, along with lignin synthesis-related NtPrx60a (Blee et al, 2003;Kavousi et al, 2010) and pathogen-inducible StPrx14 (Ros et al, 2004). In contrast, LePrx35 was clustered into subgroup C2 in which pathogen-inducible LePrx05 and CanPrx02 were also present (Vera et al, 1993;Choi et al, 2007) (Fig.…”

Section: Characteristics Of Peroxidase Genes In Tomato Fruitsmentioning

confidence: 98%

Molecular characterization of fruit-specific class III peroxidase genes in tomato ( Solanum lycopersicum )

Wang

Chan

Shien

et al. 2015

Journal of Plant Physiology

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“…Supplementary lighting allowed a photoperiod of 16 h. The temperature was between 20 and 25°C, as the temperature fell at night. Lines prx, ccr, c4h and uxs have been described previously [58,41]. Line prx contains 77 % acetyl bromide, ccr contains 58 % Klason lignin content and an increased syringyl/guaiacyl ratio as compared to NVS and c4h has an acetyl bromide lignin content of 86 % [46] A. thaliana ecotype Columbia (Col-0) and transgenic Arabidopsis plants constitutively expressing AnPGII (line 57) and overexpressing the AtPMEI-II (line 7) were previously described [35,40].…”

Section: Plant and Fungal Materialsmentioning

confidence: 99%

“…Preparation of AIM AIM of stems was isolated as previously described [39,58]. Primary walls were isolated according to [40].…”

Section: Plant and Fungal Materialsmentioning

confidence: 99%

Combination of Pretreatment with White Rot Fungi and Modification of Primary and Secondary Cell Walls Improves Saccharification

et al. 2014

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Plant cell walls have protective and structural functions conferring resistance to degradation. The lignin and hemicellulose network surrounding the cellulose microfibrils is insoluble unless subjected to harsh treatments. As lignin, pectin and xylan are effective barriers to cellulose extraction and hydrolysis, reducing their presence in cell walls improves saccharification. Microorganisms that can depolymerise lignin are of extreme interest to the biofuel industry. White rot fungi can be effective in pretreatment of lignocellulosic biomass prior to saccharification. Here, we show the cumulative effects of pretreating biomass with two white rot fungi, Phanerochaete chrysosporium and Trametes cingulata, on tobacco lines with reduced lignin or xylan, caused by suppression of the CINNAMOYL-CoA REDUCTASE, CINNAMATE-4-HYDROXYLASE, TOBACCO PEROXI-DASE 60 or UDP-GLUCURONATE DECARBOXYLASE and on Arabidopsis thaliana with reduced de-esterified homogalacturonan content, obtained by overexpressing a pectin methyl esterase inhibitor or constitutively expressing the Aspergillus nigerPOLYGALACTURONASE II gene. Tests were extended to fresh material from an Arabidopsis mutant for a cell wall peroxidase. We demonstrate that fungal pretreatment is a reliable method of improving cellulose accessibility in biofuel feedstocks, fresh material and cell wall residues from different plants. These results contribute to the understanding of the consequences of primary and secondary cell wall perturbations on lignocellulosic biomass accessibility to white rot fungi and on saccharification yield. A comparison of the effects of P. chrysosporium and T. cingulata on tobacco saccharification also highlights the limitation of current knowledge in this research field and the necessity to systematically test culture conditions to avoid generalisations.

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Consequences of antisense down-regulation of a lignification-specific peroxidase on leaf and vascular tissue in tobacco lines demonstrating enhanced enzymic saccharification

Cited by 25 publications

References 62 publications

The Arabidopsis thaliana Class III Peroxidase AtPRX71 Negatively Regulates Growth under Physiological Conditions and in Response to Cell Wall Damage.

The Arabidopsis thaliana Class III Peroxidase AtPRX71 Negatively Regulates Growth under Physiological Conditions and in Response to Cell Wall Damage.

Molecular characterization of fruit-specific class III peroxidase genes in tomato ( Solanum lycopersicum )

Combination of Pretreatment with White Rot Fungi and Modification of Primary and Secondary Cell Walls Improves Saccharification

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