Molecular characterization of fruit-specific class III peroxidase genes in tomato ( Solanum lycopersicum )

Wang, Chii-Jeng; Chan, Yuan-Li; Shien, Chin Hui; Yeh, Kai‐Wun

doi:10.1016/j.jplph.2015.01.011

Cited by 15 publications

(9 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A putative Coffea arabica class III PRX was induced in response to root-knot nematode infection [14]. Solanum lycopersicum class III PRX LePrx17 was induced by JA (jasmonic acid) and pathogen infection, and LePrx09 was induced by ethephon, SA (salicylic acid), JA, pathogen infection, wounding and H 2 O 2 stress [15]. Under arsenic (As) stress conditions, overexpression of the rice ( Oryza sativa ) class III PRX OsPRX38 in A. thaliana increased PRX, SOD (superoxide dismutase) and GST (glutathione-S-transferase) activity and enhanced lignification, resulting in reduced As accumulation [16].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide and evolutionary analysis of the class III peroxidase gene family in wheat and Aegilops tauschii reveals that some members are involved in stress responses

Yan

et al. 2019

BMC Genomics

View full text Add to dashboard Cite

Background The class III peroxidase (PRX) gene family is a plant-specific member of the PRX superfamily that is closely related to various physiological processes, such as cell wall loosening, lignification, and abiotic and biotic stress responses. However, its classification, evolutionary history and gene expression patterns are unclear in wheat and Aegilops tauschii . Results Here, we identified 374, 159 and 169 PRXs in Triticum aestivum , Triticum urartu and Ae. tauschii , respectively . Together with PRXs detected from eight other plants, they were classified into 18 subfamilies. Among subfamilies V to XVIII, a conserved exon-intron structure within the “001” exon phases was detected in the PRX domain. Based on the analysis, we proposed a phylogenetic model to infer the evolutionary history of the exon-intron structures of PRX subfamilies. A comparative genomics analysis showed that subfamily VII could be the ancient subfamily that originated from green algae ( Chlamydomonas reinhardtii). Further integrated analysis of chromosome locations and collinearity events of PRX genes suggested that both whole genome duplication (WGD) and tandem duplication (TD) events contributed to the expansion of T. aestivum PRXs ( Tae PRXs) during wheat evolution. To validate functions of these genes in the regulation of various physiological processes, the expression patterns of PRXs in different tissues and under various stresses were studied using public microarray datasets. The results suggested that there were distinct expression patterns among different tissues and PRXs could be involved in biotic and abiotic responses in wheat. qRT-PCR was performed on samples exposed to drought, phytohormone treatments and Fusarium graminearum infection to validate the microarray predictions. The predicted subcellular localizations of some Tae PRXs were consistent with the confocal microscopy results. We predicted that some Tae PRXs had hormone-responsive cis -elements in their promoter regions and validated these predicted cis -acting elements by sequencing promoters. Conclusion In this study, identification, classification, evolution, and expression patterns of PRXs in wheat and relative plants were performed. Our results will provide information for further studies on the evolution and molecular mechanisms of wheat PRXs. Electronic supplementary material The online version of this article (10.1186/s12864-019-6006-5) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 99%

Genome-wide and evolutionary analysis of the class III peroxidase gene family in wheat and Aegilops tauschii reveals that some members are involved in stress responses

Yan

et al. 2019

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…Besides, the effect of heat stress treatment on AsA content was statistically significant (Table 1). A decrease in leaf AsA content in response to high temperature was also reported in tomato plants at The POXs can be found in a broad array of organisms in various isoforms (de Oliveira et al, 2021) which have various function (stress protection and defense response) in the cell (Wang et al, 2015).…”

Section: Treatmentmentioning

confidence: 79%

Effects of Heat Stress on Some Biochemical Traits of Small Reddish Bean

Aydogan¹,

Turhan²

2022

CTNS

View full text Add to dashboard Cite

The effects of heat stress on malondialdehid (MDA) content, ascorbic acid (AsA), isoperoxidases, total soluble protein (TSP) and SDS-PAGE protein profiles of small reddish bean (Phaseolus vulgaris L. cv. Keklik) leaves were investigated. For this purpose, the collected leaf samples (preferably the third from the apex) were placed into pyrex tubes with caps closed and incubated in a water bath: after a 30-minutes habituation at 30ºC, to apply heat stress the leaves were subjected to 35, 40, 45, 50, 55 and 60°C with gradual increments every 30-minutes. According to the results, the MDA content increased with escalating temperatures, starting with 40°C. Although there was fluctuation, the AsA content generally decreased with high temperatures. Besides, TSP content decreased after 50°C. Moreover, three asidic and four basic isoperoxidase bands were identified. There was a marked difference at acidic isoperoxidase band intensities under 40 and 45°C heat treatment. Protein bands with molecu1ar weight of approximately 14.5-195 kDa were determined. In conclusion, especially 40°C is critical for small reddish bean plants, besides 40°C and above temperatures causes oxidative stress in small reddish bean plant. Additionally, isoperoxidases and proteins may be an effective mean on heat stress tolerance.

show abstract

“…It is worth noting that despite the query “ Solanum lycopersicum peroxidase” recalling 223 results on the UniProtKB database ( https://www.uniprot.org/ ) and more than 450 articles on this topic having been published from 2012 to date ( https://pubmed.ncbi.nlm.nih.gov/?term= ), very few members of this huge gene family have been recognised and characterised in tomato. Specifically, in addition to the most extensively studied peroxidases such as the two enzymes identified in tomato fruits, LePrx75 (TAP2) and LePrx76 (TAP1) 13 , 14 and the TPX1 detected in roots 15 , only four other components expressed in the fruits (LePrx09, LePrx17, LePrx35 and LePrxA) were recently characterised 16 .…”

Section: Resultsmentioning

confidence: 99%

A new versatile peroxidase with extremophilic traits over-produced in MicroTom cell cultures

Gogliettino,

Cocca,

Apone

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Peroxidases are widespread key antioxidant enzymes that catalyse the oxidation of electron donor substrates in parallel with the decomposition of H2O2. In this work, a novel tomato peroxidase, named SAAP2, was isolated from MicroTom cell cultures, purified, and characterised. The enzyme was identified with 64% sequence coverage as the leprx21 gene product (suberization-associated anionic peroxidase 2-like) from Solanum lycopersicum, 334 amino acids long. Compared to other plant peroxidases, SAAP2 was more active at elevated temperatures, with the optimal temperature and pH at 90 °C and 5.0, respectively. Furthermore, the enzyme retained more than 80% of its maximal activity over the range of 70–80 °C and the presence of NaCl (1.0–4.5 M). It also exhibited broad pH versatility (65% relative activity over the pH range 2.0–7.0), acid-tolerance (80% residual activity after 22 h at pH 2.0–7.0), high thermostability (50% residual activity after 2 h at 80 °C) and proteolytic resistance. SAAP2 exhibited exceptional resistance under thermo-acidic conditions compared to the horseradish peroxidase benchmark, suggesting that it may find potential applications as a supplement or anti-pollution agent in the food industry.

show abstract

Molecular characterization of fruit-specific class III peroxidase genes in tomato ( Solanum lycopersicum )

Cited by 15 publications

References 52 publications

Genome-wide and evolutionary analysis of the class III peroxidase gene family in wheat and Aegilops tauschii reveals that some members are involved in stress responses

Genome-wide and evolutionary analysis of the class III peroxidase gene family in wheat and Aegilops tauschii reveals that some members are involved in stress responses

Effects of Heat Stress on Some Biochemical Traits of Small Reddish Bean

A new versatile peroxidase with extremophilic traits over-produced in MicroTom cell cultures

Contact Info

Product

Resources

About