Consequences of seeded cell type on vascularization of tissue engineering constructs in vivo

Schumann, Paul; Tavassol, Frank; Lindhorst, Daniel; Stuehmer, Constantin; Bormann, Kai-Hendrik; Kampmann, Andreas; Mülhaupt, Rolf; Laschke, Matthias W.; Menger, Michael D.; Gellrich, Nils‐Claudius; Rücker, Martin

doi:10.1016/j.mvr.2009.06.003

Cited by 58 publications

(83 citation statements)

References 52 publications

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“…This observation applied to the scaffolds seeded with organ-specific cells (OLCs) or undifferentiated progenitor cells (bmMSCs) as well as to the non-vitalized scaffolds. The cell-seeded scaffolds showed a significantly higher number of capillary-like structures within their particular group (diabetic and non-diabetic) from day 6 after transplantation, a result which is in line with previous studies [8,32].…”

Section: Discussionsupporting

confidence: 91%

“…To prepare PLGA scaffolds with OLCs, cells were isolated from male C57BL/6 mice aged at 6-8 weeks as presented recently [8]. The mice were euthanized by CO2-Inhalation and washed with 70% ethanol (J.T.…”

Section: Fabrication Of Tissue-engineered Constructsmentioning

confidence: 99%

“…Bone marrow-derived murine mesenchymal stem cells were isolated according to established protocols based on plastic adherence of these cells [8,23,24]. In addition, bone marrow was obtained from male C57BL/6 mice aged at 6-8 weeks.…”

Section: Fabrication Of Tissue-engineered Constructsmentioning

confidence: 99%

“…For in vivo experiments, PLGA scaffolds were coated with collagen type I corresponding to standardized guidelines [8]. Cultivated cells were rinsed three times with HBSS and then incubated with accutase (OLCs) or Trypsin/EDTA (bmMSCs) (PAA, Coelbe, Germany) until cells were released from the culture surface.…”

Section: Fabrication Of Tissue-engineered Constructsmentioning

confidence: 99%

“…Single growth factors, especially vascular endothelial growth factor (VEGF) [3,4], have been applied as well as combinations of different growth factors [5]. A positive effect on vascularization in tissueengineered constructs has been demonstrated in co-cultures of different endothelial cell lineages and osteoblasts [6,7] and in co-cultures of bmMSCs and OLCs [6,8]. Additional strategies have been generated to prefabricate vascular structures in tissue-engineered constructs [9,10].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Decelerated vascularization in tissue-engineered constructs in association with diabetes mellitus in vivo

Schumann¹,

Lindhorst²,

Kampmann³

et al. 2015

Journal of Diabetes and its Complications

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Section: Discussionsupporting

confidence: 91%

Section: Fabrication Of Tissue-engineered Constructsmentioning

confidence: 99%

Section: Fabrication Of Tissue-engineered Constructsmentioning

confidence: 99%

Section: Fabrication Of Tissue-engineered Constructsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Decelerated vascularization in tissue-engineered constructs in association with diabetes mellitus in vivo

Schumann¹,

Lindhorst²,

Kampmann³

et al. 2015

Journal of Diabetes and its Complications

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Comparably accelerated vascularization by preincorporation of aortic fragments and mesenchymal stem cells in implanted tissue engineering constructs

Schumann

See

Kampmann

et al. 2011

J Biomedical Materials Res

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The demanding need for tissue replacement resulted in manifold approaches for the construction of different tissues. One common problem which hampers the clinical usage of tissue engineering constructs is a limited vascularization. In an attempt to accelerate the vascularization of tissue engineering constructs we compared the usage of bone marrow mesenchymal stem cells (bmMSCs) and fragments derived from the aorta in vivo. Tissue engineering constructs composed of PLGA scaffolds containing Matrigel (n = 8), aortic fragments embedded in Matrigel (n = 8), bmMSCs embedded in Matrigel (n = 8), and aortic fragments embedded in Matrigel combined with bmMSCs (n = 8) were implanted into dorsal skinfold chambers of balb/c mice and analyzed repetitively over 14 days. In all groups a weak inflammatory response was transiently apparent. Vascularization was significantly (p = 0.05) accelerated in bmMSC and aortic fragments containing constructs compared with Matrigel alone, demonstrated by a distinctly increased microvascular density throughout the whole experiment. The combination of bmMSCs and aortic fragments showed no additional effect compared with bmMSCs and aortic fragments alone. The accelerated vascularization and microvascular density of tissue engineering constructs triggered by bmMSCs and aortic fragments is comparable. Thus aortic fragments provide a new promising source for clinical relevant tissue engineering constructs.

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Accelerating the early angiogenesis of tissue engineering constructs in vivo by the use of stem cells cultured in matrigel

Schumann

Lindhorst

See

et al. 2013

J Biomedical Materials Res

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In tissue engineering research, generating constructs with an adequate extent of clinical applications remains a major challenge. In this context, rapid blood vessel ingrowth in the transplanted tissue engineering constructs is the key factor for successful incorporation. To accelerate the microvascular development in engineered tissues, we preincubated osteoblast-like cells as well as mesenchymal stem cells or a combination of both cell types in Matrigel-filled PLGA scaffolds before transplantation into the dorsal skinfold chambers of balb/c mice. By the use of preincubated mesenchymal stem cells, a significantly accelerated angiogenesis was achieved. Compared with previous studies that showed a decisive increase of vascularization on day 6 after the implantation, we were able to halve this period and achieve explicitly denser microvascular networks 3 days after transplantation of the tissue engineering constructs. Thereby, the inflammatory host tissue response was acceptable and low, comparable with former investigations. A co-incubation of osteoblast-like cells and stem cells showed no additive effect on the density of the newly formed microvascular network. Preincubation of mesenchymal stem cells in Matrigel is a promising approach to develop rapid microvascular growth into tissue engineering constructs. After the implantation into the host organism, scaffolds comprising stem cells generate microvascular capillary-like structures exceptionally fast. Thereby, transplanted stem cells likely differentiate into vessel-associated cells. For this reason, preincubation of mesenchymal stem cells in nutrient solutions supporting different steps of angiogenesis provides a technique to promote the routine use of tissue engineering in the clinic.

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Consequences of seeded cell type on vascularization of tissue engineering constructs in vivo

Cited by 58 publications

References 52 publications

Decelerated vascularization in tissue-engineered constructs in association with diabetes mellitus in vivo

Decelerated vascularization in tissue-engineered constructs in association with diabetes mellitus in vivo

Comparably accelerated vascularization by preincorporation of aortic fragments and mesenchymal stem cells in implanted tissue engineering constructs

Accelerating the early angiogenesis of tissue engineering constructs in vivo by the use of stem cells cultured in matrigel

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