Consequences of SOS1 deficiency

Abstract

As much as there is known about the function of the sodium/proton antiporter SOS1 in plants, recent studies point towards a more general role for this protein. The crucial involvement in salt stress protection is clearly one of its functions -confined to the N-terminus, but the modular structure of the protein includes a segment with several domains that are functionally not studied but comprise more than half of the protein's length. Additional functions of the protein appear to be an influence on vesicle trafficking, vacuolar pH and general ion homeostasis during salt stress. Eliminating SOS1 leads to the expression of genes that are not strictly salinity stress related. Functions that are regulated in sos1 mutants included pathogen responses, and effects on circadian rhythm.

CRISPR/Cas9-Mediated Targeted Mutagenesis of Wild Soybean (Glycine soja) Hairy Roots Altered the Transcription Profile of the Mutant

“…The expression differences of some the K + , Na + and Ca + transporters between GsSOS1 mutant and wild type were also observed, Some of the K + channel protein-coding genes SKOR and AKT1, sodium/calcium exchanger, sodium transporter HKT1, as well as 11 other intracellular traffic-related genes (Supplementary File 3). In Arabidopsis SOS1 mutant, expression of CNGC19 gene, encoding a calmodulin-binding cyclic nucleotide-gated channel, is strongly upregulated (Oh et al, 2010b). In this study, the alteration in the expression levels of four CNGC genes in GsSOS1 mutant roots was also detected.…”

“…In addition, KEGG analysis of DEGs in GsSOS1 mutant roots showed that GsSOS1 mutation significantly influenced the expression of many other genes that involved in different pathway, including hormone signal transduction, phenylpropanoid biosynthesis, starch-sucrose metabolism, circadian rhythm and plant-pathogen interactions ( Figure A4). Earlier, a few of the reports also suggest biological functions that are affected in SOS1 mutants of Arabidopsis, includes plants pathogen responses and circadian rhythm (Oh et al, 2010b). Hence, it is highly likely that the SOS1 is involved in more than one functions such as those observed in the current study.…”

“…In addition to Na + efflux, some reports also highlighted the critical roles of SOS1 in supporting vacuolar morphology, ion homeostasis, and membrane trafficking, thus mediating salt tolerance of root cells during the early stages of salt stress (Oh et al, 2010a). Further, SOS1 mutants also exhibit altered plants pathogen responses and circadian rhythm (Oh et al, 2010b). Therefore, it is highly likely that the complex structure of the large SOS1 protein is involved in more than one function.…”

Microtubule Reorganization during Mitosis and Cytokinesis: Lessons Learned from Developing Microgametophytes in Arabidopsis Thaliana

“…In dividing microspores of orchids, an MT array named generative pole MT system (GPMS) was observed between the plasma membrane and the prophase microspore nucleus prior to the assembly of the spindle (Brown and Lemmon, 1991). However, this MT array was not observed in dividing microspores expressing a green fluorescent protein (GFP)-tubulin marker in A. thaliana and tobacco (Oh et al, 2010a,c). Instead a perinuclear MT network extends to the cell cortex (Oh et al, 2010c).…”

“…In A. thaliana , immunolocalization of MTs in intact pollen grains was recently made possible (Lee et al, 2007). Moreover, a microgametophyte specific GFP-tubulin marker was introduced in tobacco and A. thaliana , so that transgenic microgametophytes are ready for the observation of MT arrays in live cells (Oh et al, 2010a,c). …”