Conservation Genetics of Threatened Hippocampus guttulatus in Vulnerable Habitats in NW Spain: Temporal and Spatial Stability of Wild Populations with Flexible Polygamous Mating System in Captivity

López, Almudena; Vera, Manuel; Planas, Miquel; Bouza, Carmen

doi:10.1371/journal.pone.0117538

Cited by 18 publications

(19 citation statements)

References 91 publications

(137 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…() and López et al. (), we challenged the initial interpretation of barriers to dispersal against the alternative hypothesis of reproductive isolation between semi‐isolated genetic backgrounds coinciding with oceanographic barriers. We describe five cryptic semi‐isolated lineages: two lineages in the Atlantic Ocean with a parapatric distribution, two lineages in the Mediterranean Sea with a patchy fine‐grained environment association (lagoon vs marine environments), and one in the Black Sea.…”

mentioning

confidence: 87%

See 1 more Smart Citation

Parallel pattern of differentiation at a genomic island shared between clinal and mosaic hybrid zones in a complex of cryptic seahorse lineages

et al. 2019

Self Cite

View full text Add to dashboard Cite

Diverging semi‐isolated lineages either meet in narrow clinal hybrid zones, or have a mosaic distribution associated with environmental variation. Intrinsic reproductive isolation is often emphasized in the former and local adaptation in the latter, although both reduce gene flow between groups. Rarely are these two patterns of spatial distribution reported in the same study system. Here, we report that the long‐snouted seahorse Hippocampus guttulatus is subdivided into discrete panmictic entities by both types of hybrid zones. Along the European Atlantic coasts, a northern and a southern lineage meet in the southwest of France where they coexist in sympatry—i.e., in the same geographical zone—with little hybridization. In the Mediterranean Sea, two lineages have a mosaic distribution, associated with lagoon‐like and marine habitats. A fifth lineage was identified in the Black Sea. Genetic homogeneity over large spatial scales contrasts with isolation maintained in sympatry or close parapatry at a fine scale. A high variation in locus‐specific introgression rates provides additional evidence that partial reproductive isolation must be maintaining the divergence. We find that fixed differences between lagoon and marine populations in the Mediterranean Sea belong to the most differentiated SNPs between the two Atlantic lineages, against the genome‐wide pattern of structure that mostly follow geography. These parallel outlier SNPs cluster on a single chromosome‐wide island of differentiation. Since Atlantic lineages do not map to lagoon‐sea habitat variation, genetic parallelism at the genomic island suggests a shared genetic barrier contributes to reproductive isolation in contrasting contexts–i.e., spatial versus ecological. We discuss how a genomic hotspot of parallel differentiation could have evolved and become associated both with space and with a patchy environment in a single study system.

show abstract

mentioning

confidence: 87%

“…, or across the Cape Finisterre oceanographic barrier, López et al. ). Four well‐differentiated genetic clusters, each distributed over extended regions, were delineated by genetic discontinuities corresponding to usual delimitations between vicariant marine lineages (Woodall et al.…”

mentioning

confidence: 99%

Parallel pattern of differentiation at a genomic island shared between clinal and mosaic hybrid zones in a complex of cryptic seahorse lineages

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Therefore, this higher similarity on bacterial fingerprints among seahorses from the same location is a good indicator that this methodological approach holds the potential to reveal local signatures and allow the tracing of the place of origin of live seahorses. Further genetic information on these wild populations of seahorses, obtained through non-lethal sampling approaches (e.g., sampling dorsal fin or skin filaments as described by López et al (2015)), will be important to validate this hypothesis in future studies. The present study also showed that bacterial communities present in the skin mucus of seahorses in the wild may vary over short-time periods (Fig.…”

Section: Resultsmentioning

confidence: 99%

First insights on the bacterial fingerprints of live seahorse skin mucus and its relevance for traceability

et al. 2018

View full text Add to dashboard Cite

A B S T R A C TDeveloping a technique to trace the geographic origin of live seahorses is paramount to increase trade regulation and foster conservation. The present study evaluated for the first time the suitability of using bacterial fingerprints present in live seahorse skin mucus to trace their origin. Bacterial 16S rDNA fragments were retrieved from seahorse mucus in a non-invasive and non-destructive way, with their profile (fingerprint) being determined using denaturing gradient gel electrophoresis (DGGE). Bacterial fingerprints were compared among seahorses: (1) originating from different geographic origins sampled at the same period; (2) originating from the same location but sampled one month apart; and (3) originating from specimens in the wild and after being stocked in captivity for 40 and 80 days. Similarities in bacterial fingerprints were determined using hierarchical cluster analysis. Results showed that geographic location affected the bacterial fingerprints of wild seahorses and that specimens sampled in the same location displayed a higher level of similarity. This finding supports that this methodological approach holds the potential to reveal local signatures and trace the origin of live seahorses. Bacterial communities from wild seahorses varied over short-time periods, with this natural variability being a potential constraint that may limit the comparison of specimens collected over long periods. Bacterial fingerprints displayed by wild specimens significantly shifted after 40 days in captivity, with a higher level of similarity being recorded for seahorses after 40 or 80 days in captivity, than when compared with those displayed in the wild. This stabilization of the bacterial community under captive conditions shows the potential that bacterial fingerprints may hold for aquaculture, as these can be used as unique signature to trace seahorses to their production facility.

show abstract

“…Each individual sample was preserved and stored in 96% ethanol for subsequent genetic analyses. Whole genomic DNA was extracted following either Woodall et al (2015), López et al (2015) or using a standard CetylTrimethyl Ammonium Bromide (CTAB) Chloroform:Isoamyl alcohol (24:1) protocol (Doyle and Doyle 1987). For samples from Vigo (Spain), the whole genome was amplified using the GenomiPhi kit (GE HealthCare) according to the manufacturer's protocol.…”

Section: Figurementioning

confidence: 99%

“…However, genetic differentiation proved to be very weak over very large geographic distances based on the two genetic studies conducted to date with microsatellite loci (e.g. from the United Kingdom to North of Spain, Woodall et al 2015, or across the Cape Finisterre oceanographic barrier, López et al 2015). Four welldifferentiated genetic clusters, each distributed over extended regions, were delineated by genetic discontinuities corresponding to usual delimitations between vicariant marine lineages (Woodall et al 2015) -between the Iberian Peninsula and the Bay of Biscay in the North Eastern Atlantic, between the Atlantic Ocean and the Mediterranean Sea, and between the Mediterranean and Black Seas.…”

Section: Introductionmentioning

confidence: 99%

Parallel pattern of differentiation at a genomic island shared between clinal and mosaic hybrid zones in a complex of cryptic seahorse lineages

Riquet

Liautard‐Haag

Woodall

et al. 2017

Preprint

Self Cite

View full text Add to dashboard Cite

33Diverging semi-isolated lineages either meet in narrow clinal hybrid zones, or have a 34 mosaic distribution associated with environmental variation. Intrinsic reproductive 35 isolation is often emphasized in the former and local adaptation in the latter, although 36 both can contribute to isolation. Rarely these two patterns of spatial distribution are 37 reported in the same study system, while this could provide fundamental information on 38 the endless debate about the relative contribution of intrinsic reproductive isolation and 39 local adaptation on the speciation process. Here we report that the low diversity long-40 snouted seahorse Hippocampus guttulatus is genetically subdivided into five cryptic 41 semi-isolated lineages. Along the Atlantic coasts, northern and southern lineages meet 42 and coexist with little hybridization in the southwest of France, forming a clinal hybrid 43 zone. In the Mediterranean Sea, two lineages have a mosaic distribution associated with 44 lagoon-like and marine habitats. A fifth lineage was identified in the Black Sea. Genetic 45 homogeneity over large spatial scales within each lineage, together with among-locus 46 . CC-BY-NC-ND 4.0 International license It is made available under a was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint (which . http://dx.doi.org/10.1101/161786 doi: bioRxiv preprint first posted online Jul. 10, 2017;. CC-BY-NC-ND4.0 International license It is made available under a was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint (which . http://dx.doi.org/10.1101/161786 doi: bioRxiv preprint first posted online Jul. 10, 2017;. CC-BY-NC-ND 4.0 International license It is made available under a was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint (which . http://dx.doi.org/10.1101/161786 doi: bioRxiv preprint first posted online Jul. 10, 2017;. CC-BY-NC-ND 4.0 International license It is made available under a was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint (which . http://dx.doi.org/10.1101/161786 doi: bioRxiv preprint first posted online Jul. 10, 2017;. CC-BY-NC-ND 4.0 International license It is made available under a was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint (which . http://dx.doi.org/10.1101/161786 doi: bioRxiv preprint first posted online Jul. 10, 2017; variance in differentiation levels between lineages and spatial patterns of introgression 47 provide evidence that partial reproductive isolation is maintaining the divergence.

show abstract

Conservation Genetics of Threatened Hippocampus guttulatus in Vulnerable Habitats in NW Spain: Temporal and Spatial Stability of Wild Populations with Flexible Polygamous Mating System in Captivity

Cited by 18 publications

References 91 publications

Parallel pattern of differentiation at a genomic island shared between clinal and mosaic hybrid zones in a complex of cryptic seahorse lineages

Parallel pattern of differentiation at a genomic island shared between clinal and mosaic hybrid zones in a complex of cryptic seahorse lineages

First insights on the bacterial fingerprints of live seahorse skin mucus and its relevance for traceability

Parallel pattern of differentiation at a genomic island shared between clinal and mosaic hybrid zones in a complex of cryptic seahorse lineages

Contact Info

Product

Resources

About