Conservation of structure and location of Rhizobium meliloti and Klebsiella pneumoniae nifB genes

Nucleotide sequence and genetic analysis of the nifB-nifQ region from Azotobacter vinelandii

“…S the predicted amino acid sequence of the translation product of the first complete ORF is compared with the amino acid sequence of the nijB gene products of K. pneumoniae (13) and Bradyrhizobium japonicum (53 …”

“…In K. pneumoniae, nijB and nifQ are contiguous and are located in the same transcriptional unit (13). The product of nifQ may be involved in reactions of molybdate and cysteine that precede the steps catalyzed by products of the FeMocofactor synthesis genes (38,39).…”

Sequence and structural organization of a nifA-like gene and part of a nifB-like gene of Herbaspirillum seropedicae strain Z78

“…5c), suggesting that transcription of nif promoters in H. seropedicae could be regulated by the NifA protein and dependent on the os4 factor (RpoN). The presence of a doublet of NifA-binding sites in the promoter region of nifB-like gene has been described before in R. meliloti (Buikema et al, 1987) and Azotobacter vinelandii (Joerger & Bishop, 1988) and may be involved in transcriptional control. This is the first nifA-like gene of a member of the Spirillaceae to be sequenced, as well as the first evidence supporting the existence of the niJA in the Spirillaceae since the work of Pedrosa & Yates (1984).…”

Physical and genetic map of the major nif gene cluster from Azotobacter vinelandii

“…Ally, and J. Beynon, Nucleic Acids Res., in press), nifH (42,47), nifD (26,42), nifK (24), ni]T (J. Beynon, M. Cannon, A. Ally, V. Buchanan-Wollaston, R. Setterquist, D. R. Dean, and F. Cannon, Nucleic Acids Res., in press), nifY (Beynon et al., in press), nifE (43), nifV (43), niJX (Beynon et al., in press), nifU (4), nifS (4), nifV (4), nifW (37; Beynon et al, in press), nifZ (37), nifM (37), nifF (18), nifL (19), nifA (14,17), nifB (13), and nifQ (13), has been determined. In addition to the work cited above, the nucleotide sequence of the K. pneumoniae nif cluster was also determined independently (W. Arnold, A. Rump, W. Klipp, V. B. Priefer, and A. Puhler, J. Mol.…”