Conservation of the Protected Resurection SpeciesRamonda SerbicaPanč.—Habitat Montana District, Bulgaria asIn Vitro, Plants Through a Modified Micropropagation System

Dontcheva, S.; Daskalova, Evelina; Yahubyan, Galina; Denev, Iliya; Minkov, Ivan; Toneva, Valentina

doi:10.1080/13102818.2009.10818441

Cited by 12 publications

(11 citation statements)

References 7 publications

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“…It appears that the phytohormones are not necessary for seed germination medium for oligotrophic seeds of Ramonda plants. The same results were obtained from the experiments carried out with R. serbica seeds of Bulgarian area (Dontcheva et al 2009;Daskalova et al 2012). The authors have observed a normal organogenesis of individual plantlets from germinated seeds in the nutrient medium GM without sugar and phytohormones.…”

Section: Direct Organogenesis Stage 1 (Seed Germination)supporting

confidence: 77%

Micropropagation and in vitro conservation of the rare and threatened plants Ramonda serbica and Ramonda nathaliae

Gashi

Abdullai

Sota

et al. 2014

Physiol Mol Biol Plants

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Ramonda serbica and Ramonda nathaliae are rare and endemo relict plant species from Balkan Peninsula. An efficient micro propagation and in vitro conservation method via direct and indirect organogenesis from seed and leaf explants, respectively, was established in this study. The seed of both Ramonda species were collected from different populations in Kosovo, and were germinated in nutrient media JG-B without any phytohormone. The highest number of shoots and multiplication rate was observed on JG-B medium supplemented with BAP and IAA (0.5 mg l −1 each), whereas the highest number of leaves per plantlets was found on WPM and RA medium supplemented with BAP and IAA (0.1 mg l −1 each). During this stage of micro propagation some significant differences were observed in plantlets from different populations. The indirect organogenesis from parts of leaves of natural plants was not successful due to unavailability of established protocol for disinfections of the plant material. On other hand, parts of leaves from micro propagated plantlets, cultured on MS medium supplemented with different ratio of BAP and NAA, resulted in the highest efficiency for shoot regeneration. In vitro conservation of micro propagated plants at the lower temperature (4°C) had a significantly positive effect for storage of more than 12 months.

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Section: Direct Organogenesis Stage 1 (Seed Germination)supporting

confidence: 77%

Micropropagation and in vitro conservation of the rare and threatened plants Ramonda serbica and Ramonda nathaliae

Gashi

Abdullai

Sota

et al. 2014

Physiol Mol Biol Plants

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“…Previous investigation of R. serbica and R. nathaliae has brought the evidence to adaptive features necessary to preserve cell membrane integrity (Quartacci et al 2002), antioxidative capacity (Sgherri et al 2004;Jovanoviç et al 2011), photosynthetic activity (Tuba et al 1998), CO 2 fixation and chlorophyll a fluorescence (Degl'Innocenti et al 2008), genome size variation and polyploidy (Siljak-Yakovlev et al 2008), osmotic adjustment (Zivkoviç et al 2005), seed germination (Gashi et al 2012a) and in vitro cultivation from seeds of Ramonda plants (Kongjika et al 2002;Gashi et al 2011;Dontcheva et al 2009;Daskalova et al 2012). …”

Section: Introductionmentioning

confidence: 99%

Chlorophyll fluorescence imaging of photosynthetic activity and pigment contents of the resurrection plants Ramonda serbica and Ramonda nathaliae during dehydration and rehydration

Gashi

Babani

Kongjika

2013

Physiol Mol Biol Plants

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The desiccation-tolerant plants of the R. serbica and R. nathaliae are resurrection plants which are able to fully recover their physiological function after anabiosis. A comparison of chlorophyll fluorescence imaging and photosynthetic pigment contents responses of R. serbica and, for the first time, R. nathaliae to dehydration and rehydration were investigated. For this purpose, plants after collection from their natural habitats were kept fully watered for 14 days at natural condition. The experiment was conducted with mature leaves of both species. R. serbica and R. nathaliae plants were dehydrated to 5.88 % and 7.87 % relative water content (RWC) by withholding water for 15 days, afterwards the plants were rehydrated for 72 hours to 94.67 % and 97.02 % RWC. During desiccation, R. serbica plants preserved the chlorophyll content about 84 %, while R. nathaliae about 90 %. During dehydration when RWC were more than 40 %, photochemical efficiency of PSII for photochemistry, the Fv/Fm ratio, decreased about 40 % in R. nathaliae plants, but a strong reduction with 60 % was recorded for R. serbica. Following rehydration, the Fv/Fm ratio recovered more rapidly in R. nathaliae. The higher photosynthetic rates could also be detected via imaging the chlorophyll fluorescence decrease ratio Rfd, which possessed higher values after rehydration leaves of R.nathaliae as compared to R. serbica. The results showed that the photosynthetic activity and chlorophyll contents after rehydration are recovered more rapidly in R. nathaliae in comparison to R. serbica.

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“…This research has brought the evidence of adaptive features necessary to preserve cell membrane integrity [4], antioxidative capacity [5,6], photosynthetic activity [1], CO 2 fixation and chlorophyll a fluorescence [7], genome size variation and polyploidy [8], osmotic adjustment [9], seed germination [10] and in vitro cultivation from seeds of Ramonda plants [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Photosynthetic Pigment Contents of the Resurrection Plants Ramonda serbica and Ramonda nathaliae of Some Different Populations from Kosovo, Albania and Macedonia

Gashi¹,

Abdullai²,

Kongjika³

2012

AJPS

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The resurrection plants Ramonda serbica and Ramonda nathaliae are the physiological model plant and endemic species from Balkan Peninsula. Study was carried out to assess the impact of different populations on photosynthetic pigment contents and their effect on physiological activity of these species. The experiment was conducted with leaves of R. serbica collected from nine populations in Kosovo, three in Albania and two in Macedonia, while for R. nathaliae four populations in Macedonia. For this purpose plants after collection from their natural populations were kept for 24 hours at constant temperature and humidity and in natural photoperiod. According to our data the pigment contents (Total chl + Carot) of R. serbica, measured as mg per gram dry weight (DW), were higher (7.06 mg·g −1 DW) in Radaci populations (Kosovo) and lower (4.63 mg·g −1 DW) in Jukniu Mountain-Kruja (Albania). On the other hand, the higher (5.28 mg·g −1 DW) of pigment contents (Total chl + Carot) of R. nathaliae were observed in Vorca populations and the lower (4.24 mg·g −1 DW) in Kaparllëk populations. Ratio chlorophyll a/b of R. serbica in Kruja Castle populations from Albania was the higher (3.68) comparing with Zhlebi populations from Kosovo (1.68). Similarly in case of R. nathaliae the higher (3.36) value was in Kaparllëk populations and the lower (3.12) in Matka populations. The data obtained were further analyzed using one-way ANOVA and a significant change was recorded in the different populations. These studies clearly indicate that the Ramonda plants from different ecological habitats there have been changes of photosynthetic pigment contents.

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Conservation of the Protected Resurection SpeciesRamonda SerbicaPanč.—Habitat Montana District, Bulgaria asIn Vitro, Plants Through a Modified Micropropagation System

Cited by 12 publications

References 7 publications

Micropropagation and in vitro conservation of the rare and threatened plants Ramonda serbica and Ramonda nathaliae

Micropropagation and in vitro conservation of the rare and threatened plants Ramonda serbica and Ramonda nathaliae

Chlorophyll fluorescence imaging of photosynthetic activity and pigment contents of the resurrection plants Ramonda serbica and Ramonda nathaliae during dehydration and rehydration

Comparison of Photosynthetic Pigment Contents of the Resurrection Plants <i>Ramonda serbica</i> and <i>Ramonda nathaliae</i> of Some Different Populations from Kosovo, Albania and Macedonia

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Conservation of the Protected Resurection SpeciesRamonda SerbicaPanč.—Habitat Montana District, Bulgaria asIn Vitro, Plants Through a Modified Micropropagation System

Cited by 12 publications

References 7 publications

Micropropagation and in vitro conservation of the rare and threatened plants Ramonda serbica and Ramonda nathaliae

Micropropagation and in vitro conservation of the rare and threatened plants Ramonda serbica and Ramonda nathaliae

Chlorophyll fluorescence imaging of photosynthetic activity and pigment contents of the resurrection plants Ramonda serbica and Ramonda nathaliae during dehydration and rehydration

Comparison of Photosynthetic Pigment Contents of the Resurrection Plants &lt;i&gt;Ramonda serbica&lt;/i&gt; and &lt;i&gt;Ramonda nathaliae&lt;/i&gt; of Some Different Populations from Kosovo, Albania and Macedonia

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Comparison of Photosynthetic Pigment Contents of the Resurrection Plants <i>Ramonda serbica</i> and <i>Ramonda nathaliae</i> of Some Different Populations from Kosovo, Albania and Macedonia