Micropropagation and in vitro conservation of the rare and threatened plants Ramonda serbica and Ramonda nathaliae

Abstract

Ramonda serbica and Ramonda nathaliae are rare and endemo relict plant species from Balkan Peninsula. An efficient micro propagation and in vitro conservation method via direct and indirect organogenesis from seed and leaf explants, respectively, was established in this study. The seed of both Ramonda species were collected from different populations in Kosovo, and were germinated in nutrient media JG-B without any phytohormone. The highest number of shoots and multiplication rate was observed on JG-B medium supplemented with BAP and IAA (0.5 mg l −1 each), whereas the highest number of leaves per plantlets was found on WPM and RA medium supplemented with BAP and IAA (0.1 mg l −1 each). During this stage of micro propagation some significant differences were observed in plantlets from different populations. The indirect organogenesis from parts of leaves of natural plants was not successful due to unavailability of established protocol for disinfections of the plant material. On other hand, parts of leaves from micro propagated plantlets, cultured on MS medium supplemented with different ratio of BAP and NAA, resulted in the highest efficiency for shoot regeneration. In vitro conservation of micro propagated plants at the lower temperature (4°C) had a significantly positive effect for storage of more than 12 months.

A high-frequency in vitro multiplication, micromorphological studies and ex vitro rooting of Cadaba fruticosa (L.) Druce (Bahuguni): a multipurpose endangered medicinal shrub

“…Thus an effective strategy is required for rapid and large-scale multiplication of this potential medicinal plant. During last 2-3 decades, tissue culture methods involving micropropagation has been widely applied to propagate and conserve a number of endangered and medicinally important plant species (Rathore et al 2005;Lodha et al 2014a;Gashi et al 2015). reported basic tissue culture protocol for conservation of one of the Cadaba species (C. heterotricha).…”

Micropropagation of Medicinal Plants: a Review

“…The situation warrants acceleration of efforts to develop methods for their germplasm preservation. Given their vital importance, we must conserve them for the benefit of both present and future generations (Gashi et al, 2015).…”

Conservación de plantas regeneradas in vitro y análisis de la variación somaclonal de Cinchona officinalis, Linneo

“…Se reportan con mayor frecuencia: i. La disminución de temperatura Gonçalves & Romano, 2007;Catana et al 2010;Marino et al 2010;Capuana & Di Leonardo 2013;Gashi et al 2015), ii. La modificación de los nutrientes del medio de cultivo (Taylor & Dukic 1993;Akdemir et al 2013;Nogueira et al 2014;Mendonça et al 2015) y iii.…”

“…(Smeekens 2000;Leva et al 2013). Al existir una disminución de la concentración de los componentes del medio se ocasiona una reducción del metabolismo (Catana et al 2010;Gashi et al 2015). Tradicionalmente una de las formulaciones más completas para el crecimiento de tejidos in vitro ha sido el medio Murashige and Skoog (1962), éste se caracteriza por la mezcla de sales y en especial por el elevado contenido de nitrógeno y sus complementos vitamínicos.…”