Conservative Micropropagation of Some Endemic or Rare Species From the Dianthus Genus

Cristea, Vasile; Pușcaș, Mihai; Miclaus, Mihai; Deliu, Constantin

doi:10.17660/actahortic.2006.725.46

Cited by 10 publications

(15 citation statements)

References 3 publications

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“…Our results regarding root induction are comparable with those obtained for the same taxon by Cristea et al (2006). They obtained 11 roots/inoculum after 56 days of culture on medium supplemented with K and NAA (1.0 mg/L each).…”

Section: In Vitro Multiplicationsupporting

confidence: 90%

Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers

Jarda¹,

Butiuc-Keul²,

Höhn³

et al. 2014

Turk J Biol

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Ex situ conservation of Dianthus giganteus subsp. banaticus was obtained by in vitro culture on different culture media supplemented with phytohormones. Assessment of somaclonal variability over a period of preservation by in vitro culture is required prior to introduction and acclimatization in natural habitats. In this study we used 2 types of molecular markers, ISSR and multilocus SSR markers, which allow comparison of different alleles, to assess the somaclonal variability of endemic taxa Dianthus giganteus subsp. banaticus after a period of 24 months of preservation by in vitro culture. The assessment of somaclonal variability of D. giganteus subsp. banaticus shows some changes in the absence/presence of certain markers in some individuals.

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Section: In Vitro Multiplicationsupporting

confidence: 90%

Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers

Jarda¹,

Butiuc-Keul²,

Höhn³

et al. 2014

Turk J Biol

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“…Анализирајући истраживања приказана у табели 1, можемо уочити да је код већине таксона иницијална култура успостављена сетвом семена in vitro чиме се постиже очување генетске варијабилности (Pence, 1999, Johnson, 2002 Catana et al, 2010, Paunescu, Holobiuc, 2003, Cristea et al, 2004, Holobiuc i Blindu, 2006, Holobiuc et al, 2009 Pyrenees Mts., Cristea et al, 2006a, Marcu et al, 2006 D. serotinus Waldst. & Kit.…”

Section: размножавање угрожених врста каранфилаunclassified

“…Pannonian endemit Marković et al, 2007D. spiculifolius Schur Carpathians (Butiuc-Keul et al, 2001, Cristea et al, 2002, 2006b,b, Holobiuc i Blindu, 2006, Holobiuc et al, 2009, 2010b, Pop, Pamfil, 2011 Код свих наведених таксона, превасходно је испитивано дејство различитих врста и концентрација фитохормона на развој у култури in vitro. Међутим, код неких таксона су спровођена додатна истраживања.…”

Section: размножавање угрожених врста каранфилаunclassified

“…Такође, спроведена су и истраживања унапређења постојећих протокола микропропагације D. tenuifolius, D. callizonus, D. spiculifolius, D. superbus ssp. speciosus (Cristea et al, 2004, Holobiuc i Blindu, 2006, а код D. spiculifolius испитана је генетска стабилност биљака добијених in vitro, при чему је утврђено да је генетска варијабилност популације сачувана применом одговарајућег протокола микропропагације, док је сомаклонално варирање in vitro биљака минимално (Cristea et al, 2014). Код врсте D. serotinus је први пут код рода Dianthus испитано дејство различитих врста и концентрација шећера на развој култура у фази мултипликације, а с обзиром да расте на песку алкалне реакције, истражен је и утицај pH вредности медијума (Marković et al, 2013a).…”

Section: размножавање угрожених врста каранфилаunclassified

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In vitro propagation of endangered Dianthus taxa

Marija¹,

Mihailo²,

Đukić³

2015

Glas Suma Fakul

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The review of recent researches regarding the in vitro culture of 30 endangered Dianthus taxa is presented in this paper. Various in vitro protocols developed for selected rare and threatened Dianthus taxa are analysed in order to provide a useful synthesis of the data obtained with the main principles, techniques and recommendations for futher research and practice. The recapitulated data presented in this review can be used as a tool for the micropropagation of other endangered Dianthus taxa, enabling their propagation and obtaining a sufficient amount of plants for reintroduction. In addition, the obtained results represent the basis for ex situ conservation of the investigated taxa, especially for medium-term and long-term conservation (cryopreservation). [Projekat Ministarstva nauke Republike Srbije, br. 43007]

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“…The already published studies regarding the in vitro multiplication of the analyzed species (Butiuc-Keul et al, 2001;Cristea et al, 2002Cristea et al, , 2006Holobiuc and Blîndu, 2006) do not contain details on the ex situ acclimatization. The results presented here on the ex situ acclimatization of in vitro generated plantlets meet the expectations, having a superior efficiency.…”

Section: Ex Situ Outdoor Acclimatization Of Plantletsmentioning

confidence: 99%

Ex situ Conservation of Three Endemic and/or Endangered Dianthus Species

Cristea

Jarda

Holobiuc³

2013

Not Bot Hort Agrobot Cluj

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Within the current context of declining biodiversity, the botanical gardens play an essential role in its conservation. Dianthus callizonus, D. glacialis ssp. gelidus and D. spiculifolius are the species that we seek to preserve in "Alexandru Borza" Botanical Garden of Cluj-Napoca (Romania). Several replicates were collected for each taxon from different populations in order to avoid the genetic uniformity. The material collected from the natural sites, was planted on a rockery, specially designed for this collection in the Botanical Garden. At the time of planting, each individual was sampled for setting up an in vitro collection and further biochemical and molecular analyses. In case of ex situ outdoor conservation of the three Dianthus species, 80.6% of the individuals collected in the field survived during the first year but the percentage decreased drastically after four years. In the case of in situ collected individuals, as well as in the case of in vitro individuals, D. spiculifolius had the best ability to acclimatize in the Botanical Garden, and D. callizonus presented the lowest number of surviving individuals. The ex vitro acclimatization of the plantlets had 80% efficiency at 10ºC, using three different substrates: soil and pearl stone mix 1/1, soil and sand mix 1/1 and pearl stone. All the three species are preserved in vitro, whereas the plantlets are acclimatized outdoors. Ex situ conservation of these species will have a positive impact on the biodiversity conservation.

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Conservative Micropropagation of Some Endemic or Rare Species From the Dianthus Genus

Cited by 10 publications

References 3 publications

Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers

Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers

In vitro propagation of endangered Dianthus taxa

Ex situ Conservation of Three Endemic and/or Endangered Dianthus Species

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