Considerations for the Use of Clostridial Collagenase in Clinical Practice

Jung, Won‐Kyo; Winter, H.

doi:10.2165/00044011-199815030-00009

Cited by 31 publications

(27 citation statements)

References 21 publications

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“…31 A very high proportion of the skin (70-80% of its dry weight) consists of collagen, and the action of collagenases on damaged skin during wound repair is of importance. 32 Clostridyum collagenase is sometimes used clinically to debride or clean the wound of necortic tissue at the edges of the wound bed so that healing can take place. 33 However, it is now recognized that chronic wounds require modulation of MMP levels with proteaselowering dressings.…”

Section: Protease-lowering Effect Of Dressingsmentioning

confidence: 99%

Human neutrophil elastase and collagenase sequestration with phosphorylated cotton wound dressings

Edwards

Howley

2007

J Biomedical Materials Res

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The design and preparation of wound dressings that redress the protease imbalance in chronic wounds is an important goal of wound healing and medical materials science. Chronic wounds contain high levels of tissue and cytokine-destroying proteases including matrix metalloprotease and neutrophil elastase. Thus, the lowering of excessive protease levels in the wound environment by wound dressing sequestration prevents the breakdown of extracellular matrix proteins and growth factors necessary for wound healing. Phosphorylated cotton wound dressings were prepared to target sequestration of proteases from chronic wound exudate through a cationic uptake binding mechanism involving salt bridge formation of the positively charged amino acid side chains of proteases with the phosphate counterions of the wound dressing fiber. Dressings were prepared by applying sodium hexametaphosphate and diammonium phosphate in separate formulations to cotton gauze by pad/dry/cure methods. Phosphorylated cotton dressings were assessed for their ability to lower elastase and collagenase activity. The phosphorylated cotton dressings lowered elastase and collagenase activity 40-80% more effectively than the untreated cotton wound dressings under conditions that mimic chronic wound exudate. Efficacy of the phosphorylated cotton was found to be related to the level of phosphorylation and a lower pH due to protonated phosphate at the surface of the dressing. The capacity of the modified gauze to sequester continued elastase secretions similar to that found in a chronic wound over a 24-h period was retained within a 80% retention of elastase sequestration and was dose-dependent.

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Section: Protease-lowering Effect Of Dressingsmentioning

confidence: 99%

Human neutrophil elastase and collagenase sequestration with phosphorylated cotton wound dressings

Edwards

Howley

2007

J Biomedical Materials Res

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“…Thus, in contrast to the vertebrate collagenases, ChC degrades collagen into small peptides. 127,130 ChC could not be crystallized for the moment, and its three-dimensional structure is thus not available. Only recently some electronic spectroscopic studies of Co(II)-substituted ChC have been reported, 131 offering thus interesting information regarding the binding of inhibitors within the active site of this bacterial protease.…”

Section: B Metalloproteases Of the M9 Family (Vibrio And Clostridiummentioning

confidence: 99%

Bacterial protease inhibitors

Supuran

Scozzafava

Clare

2002

Medicinal Research Reviews

149

120

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Serine-, cysteine-, and metalloproteases are widely spread in many pathogenic bacteria, where they play critical functions related to colonization and evasion of host immune defenses, acquisition of nutrients for growth and proliferation, facilitation of dissemination, or tissue damage during infection. Since all the antibiotics used clinically at the moment share a common mechanism of action, acting as inhibitors of the bacterial cell wall biosynthesis or affecting protein synthesis on ribosomes, resistance to these pharmacological agents represents a serious medical problem, which might be resolved by using new generation of antibiotics, possessing a different mechanism of action. Bacterial protease inhibitors constitute an interesting such possibility, due to the fact that many specific as well as ubiquitous proteases have recently been characterized in some detail in both gram-positive as well as gram-negative pathogens. Few potent, specific inhibitors for such bacterial proteases have been reported at this moment except for some signal peptidase, clostripain, Clostridium histolyticum collagenase, botulinum neurotoxin, and tetanus neurotoxin inhibitors. No inhibitors of the critically important and ubiquitous AAA proteases, degP or sortase have been reported, although such compounds would presumably constitute a new class of highly effective antibiotics. This review presents the state of the art in the design of such enzyme inhibitors with potential therapeutic applications, as well as recent advances in the use of some of these proteases in therapy.

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“…Apresentam massas molares entre 30.000 e 150.000 Da. Como todos os membros das MMPs, são enzimas Zn 2+ dependentes, sendo inibidas por qualquer quelante que se ligue a esse íon, tendo a necessidade do Ca 2+ para manutenção da sua estabilidade (Jung and Winter, 1998, Daboor et al, 2012, Daboor et al, 2010. Apenas as MMP 1, 8, 13, 14 e 18 possuem a atividade de decompor os tipos de colágeno nativo de cadeia tripla I, II, III, VII e X (Daboor et al, 2010, Tallant et al, 2010, Araújo et al, 2011.…”

Section: Metalocolagenasesunclassified

“…Os fibroblastos são atraídos para a ferida por fatores segregados a partir de macrófagos. Eles produzem colágeno novo para a reconstrução da matriz extracelular (Jung and Winter, 1998). As colagenases aumentam a proliferação, a angiogênese e a migração das células dérmicas, removendo os tecidos necróticos, mas não danificando o tecido saudável (Alipour et al, 2016 Existem inúmeras vantagens do uso de colagenases no tratamento de feridas: a) removem o tecido necrosado com maior eficiência por sua capacidade de hidrolisar vários tipos de colágeno; b) são indolores e não-hemorrágicos; c) podem ser usados por longos períodos e também em associação com outros medicamentos; d) atraem macrófagos e fibroblastos para o local da ferida; e) aumentam a formação de tecidos de granulação e estimulam o próprio organismo a promover a cicatrização (Jung and Winter, 1998).…”

Section: Aplicações Na Indústria Biomédica E Terapêuticaunclassified

“…Eles produzem colágeno novo para a reconstrução da matriz extracelular (Jung and Winter, 1998). As colagenases aumentam a proliferação, a angiogênese e a migração das células dérmicas, removendo os tecidos necróticos, mas não danificando o tecido saudável (Alipour et al, 2016 Existem inúmeras vantagens do uso de colagenases no tratamento de feridas: a) removem o tecido necrosado com maior eficiência por sua capacidade de hidrolisar vários tipos de colágeno; b) são indolores e não-hemorrágicos; c) podem ser usados por longos períodos e também em associação com outros medicamentos; d) atraem macrófagos e fibroblastos para o local da ferida; e) aumentam a formação de tecidos de granulação e estimulam o próprio organismo a promover a cicatrização (Jung and Winter, 1998). Na medicina veterinária, já foram empregadas para quebrar a rede fibromuscular para isolamento de células espermatogênicas do tecido testicular em equinos (Jung and Yoon, 2016), em casos de tendinite equina (Wallis et al, 2010, Oloumi et al, 2011 e no isolamento de células-tronco mesenquimais em caninos (Lee et al, 2013).…”

Section: Aplicações Na Indústria Biomédica E Terapêuticaunclassified

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Colagenasas do pescado y sus aplicaciones industriales

Oliveira

Cunha

Assis

et al. 2017

Pubvet

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RESUMO.Colagenases são grupos de enzimas capazes de clivar a tripla-hélice do colágeno não tendo ação sobre outro tipo de proteína. São obtidas a partir de uma vasta gama de fontes, tais como tecido animal e vegetal. Uma fonte promissora na atualidade é a partir de resíduos negligenciados do processamento do pescado, tais como vísceras internas (intestino). Esta revisão objetivou realizar um levantamento quantitativo da produção cientifica relacionada à colagenase do pescado, bem como dissertar sobre as principais características bioquímicas e aplicações industriais dessa protease, com ênfase nas colagenases extraídas a partir de organismos aquáticos. Dessa forma, através de análise do Scopus, no período de 1963 a 2016, foram detectadas 274 citações ao termo "collagenase fish". A produção cientifica pertinente a colagenase do pescado está intrinsicamente relacionada a necessidade industrial de fontes alternativas dessas proteases colagenolíticas, devido sua extensa aplicação industrial, tais como nos segmentos alimentícios, biomédicos, terapêuticos e têxtil.Palavras chave: Biotecnologia, enzimas, pescado, protease, resíduos Fish collagenases and its industrial applicationsABSTRACT. Collagenases are a group of enzymes capable of cleaving the triple-helix collagen, having no action on another type of protein. They are obtained from a wide range of sources, such as animal and plant tissue. A promising source at present is from neglected fish processing residues, such as internal viscera (intestine). This review aimed to quantify the scientific production related to fish collagenase, as well as to discuss the main biochemical characteristics and industrial applications of this protease, with emphasis on collagenases extracted from aquatic organisms. Thus, through analysis of Scopus, from 1963 to 2016, 274 citations were detected to the term "collagenase fish". The scientific production relevant to fish collagenase is intrinsically related to the industrial need for alternative sources of these collagenolytic proteases, due to their extensive industrial application, such as in the food, biomedical, therapeutic and textile segments.

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Considerations for the Use of Clostridial Collagenase in Clinical Practice

Cited by 31 publications

References 21 publications

Human neutrophil elastase and collagenase sequestration with phosphorylated cotton wound dressings

Human neutrophil elastase and collagenase sequestration with phosphorylated cotton wound dressings

Bacterial protease inhibitors

Colagenasas do pescado y sus aplicaciones industriales

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