Consistent amounts of acute leukemia-associated P190BCR/ABL transcripts are expressed by chronic myelogenous leukemia patients at diagnosis

Saglio, Giuseppe; Pane, Fabrizio; Gottardi, Enrico; Frigeri, Ferdinando; Buonaiuto, Maria Rosaria; Guerrasio, Angelo; Micheli, Daniela De; Parziale, Adele; Fornaci, Maria Nella; Martinelli, Giovanni; Salvatore, Francesco

doi:10.1182/blood.v87.3.1075.bloodjournal8731075

Cited by 96 publications

(33 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In some of our samples multiple intermediate PCR products were produced which were most probably the result of alternative splicing of the BCR-ABL pre-mRNA. We believe that the p190 transcripts in our samples as well as in the samples, examined by others (Saglio et al, 1996;van Rhee et al, 1996), were the result of alternative splicing. Six of the co-expressing patient samples included in this study were also analysed by interphase FISH using BCR and ABL probes, and in each case only a single Ph chromosome was seen per cell (data not shown).…”

Section: Discussionsupporting

confidence: 51%

“…The expression of p190 transcripts in chronic-phase CML patient samples has been reported previously (Saglio et al, 1996;van Rhee et al, 1996). In their studies co-expression of p190-and p210-encoding BCR-ABL transcripts occurred in >70% of patients with chronic-phase CML.…”

Section: Discussionmentioning

confidence: 84%

“…The p190-encoding transcript was detected at lower levels than the p210-encoding transcript, probably not only due to lower expression levels but also due to competition for primers between the various BCR-ABL transcripts present in the co-expressing samples. In their study Saglio et al (1996) reported the detection by RT-PCR (1996), using a very similar RT-PCR technique, more recently reported that only 0·02% of the fusion transcripts present at diagnosis were of the p190-encoding type. It should be noted that Saglio et al (1996) were able to detect not only the p190 message but also the p190 BCR-ABL protein in patient samples, lending support to their estimation of relative levels.…”

Section: Discussionmentioning

confidence: 99%

“…In their study Saglio et al (1996) reported the detection by RT-PCR (1996), using a very similar RT-PCR technique, more recently reported that only 0·02% of the fusion transcripts present at diagnosis were of the p190-encoding type. It should be noted that Saglio et al (1996) were able to detect not only the p190 message but also the p190 BCR-ABL protein in patient samples, lending support to their estimation of relative levels. Although the expression level of the p190 transcript is clearly lower than that of the p210 transcript, the best means by which to resolve whether the presence of the p190 transcript is of pathological importance may be to compare clinical parameters between these two patient groups.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Expression of p210 and p190 BCR‐ABL due to alternative splicing in chronic myelogenous leukaemia

et al. 1998

View full text Add to dashboard Cite

Summary. The hallmark of chronic myelogenous leukaemia (CML) is the presence of the Philadelphia chromosome and its resultant fusion message, BCR-ABL, and fusion protein, p210. Patients with CML in blast crisis, or with Philadelphia positive acute lymphoblastic leukaemia (ALL), can have a smaller BCR-ABL fusion transcript possessing only the first exon of BCR fused to ABL. This smaller transcript encodes a 190 kD protein which is more strongly transforming than the p210 protein derived from the larger CML-associated transcript. We performed RT-PCR on samples from CML patients in chronic phase to determine the frequency and mechanism of p190 and p210 co-expression and to see if this correlated with clinical indices.We examined the peripheral blood or marrow of 67 patients with CML and found that 35 of them expressed both transcripts whereas the remainder expressed the p210-encoding transcript exclusively. Additional PCR products of an intermediate size were also frequently detected and have been isolated and sequenced. Data from two of these products indicate that they are the result of alternative splicing and include variable combinations of BCR exons. We believe that the expression of the p190-encoding transcript in the chronic phase of CML is also due to alternative splicing. A comparison of patients co-expressing the p190-and p210-encoding transcripts with those patients who expressed only the p210-encoding transcript detected significantly higher white blood cell (WBC) counts and blast cell counts at time of testing as well as significantly higher white blood cell counts at diagnosis.

show abstract

Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Expression of p210 and p190 BCR‐ABL due to alternative splicing in chronic myelogenous leukaemia

et al. 1998

View full text Add to dashboard Cite

show abstract

“…Dhingra et al (1991) have speculated that co-existence of P190 BCR-ABL and P210 BCR-ABL type mRNAs might correlate with disease progression. However, Saglio et al (1996) have recently reported that co-expression of CML and ALL type mRNA in CML is actually very frequent and is not correlated with disease progression.…”

Section: Discussionmentioning

confidence: 99%

A novel acute lymphoid leukaemia type BCR/ABL transcript in chronic myelogenous leukaemia

et al. 1997

View full text Add to dashboard Cite

Summary. Using a reverse transcription-polymerase chain reaction (RT-PCR), we identified a patient with typical clinical features of chronic myelogenous leukaemia (CML) in the chronic phase who showed no amplification of the CML-type BCR/ABL transcript. RT-PCR with primers detecting the acute lymphoid leukaemia (ALL)-type transcript disclosed a novel fragment co-amplified with an ALL-type fragment. Sequencing revealed the novel transcript to be a chimaeric mRNA produced by fusion of a segment of BCR exon 2 (e2) to ABL exon 2 (a2), with a 21 base-pair insertion of ABL intron 1b sequence between them. This transcript has not been reported previously.

show abstract

Hyperfunction of neutrophils in a patient with BCR/ABL negative chronic myeloid leukemia

Watari

Tojo

Nagamura‐Inoue

et al. 2000

Cancer

View full text Add to dashboard Cite

Consistent amounts of acute leukemia-associated P190BCR/ABL transcripts are expressed by chronic myelogenous leukemia patients at diagnosis

Abstract: Supported by grants from MURST, CNR (Progetto jinalizzato FATMA Sp I , and Progettojnalizzato ACRO Sp 4, Rome), Regione Campania, AIRC (Milan) and Agensud. A.D. is supported by an AIDS fellowship of Minister0 della Sanita.Address reprint requests to

Cited by 96 publications

References 14 publications

Expression of p210 and p190 BCR‐ABL due to alternative splicing in chronic myelogenous leukaemia

Expression of p210 and p190 BCR‐ABL due to alternative splicing in chronic myelogenous leukaemia

A novel acute lymphoid leukaemia type BCR/ABL transcript in chronic myelogenous leukaemia

Hyperfunction of neutrophils in a patient with BCR/ABL negative chronic myeloid leukemia

Contact Info

Product

Resources

About