Constituents of <i>Melittis melissophyllum</i> subsp. <i>albida</i>

Venditti, Alessandro; Frezza, Claudio; Caretti, Fulvia; Gentili, Alessandra; Serafini, Mauro; Bianco, Armandodoriano

doi:10.1177/1934578x1601101101

Cited by 2 publications

(2 citation statements)

References 30 publications

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“…Currently, M. melissophyllum is used for the purpose of aromatizing alcohol beverages and tobacco products [8]. The herb is rich in phenolic compounds, namely coumarin and its derivatives, flavonoids and phenolic acids also contains a small amount of essential oil [9][10][11][12][13][14][15]. The following biologically active compounds have been previously detected in M. melissophyllum: flavonoids, including myricetin, quercetin, luteolin, kaempferol, apigenin, as well as some phenolic acids, such as protocatechuic, chlorogenic, p-hydroxybenzoic, vanillic, caffeic, syringic, p-coumaric, ferulic, sinapic, o-coumaric, cinnamic acids [13,14].…”

Section: Introductionmentioning

confidence: 99%

Effect of Shading on Development, Yield and Quality of Bastard Balm Herb (Melittis melissophyllum L.)

et al. 2020

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The aim of the study was to assess the effects of Melittis melissophyllum shading on its development and accumulation of phenolics. Their content (verbascoside, apiin, luteolin-7-O-glucoside, coumarin, 3,4-dihydroxycoumarin, o-coumaric acid 2-O-glucoside as well as o-coumaric, p-coumaric, chlorogenic, caffeic, ferulic and cichoric acid) was determined in the herb using HPLC-DAD. The results showed that the content of abovementioned flavonoids and phenolic acids was highest in plants grown under full sunlight. On the other hand, a higher content of coumarin was observed in shaded plants, especially after the seed-setting stage. A similar tendency was noted for the amount of chlorophyll a and b. The content of hydrogen peroxide and malondialdehyde, the activity of polyphenol oxidase and catalase and the antioxidant capacity of plant extracts (measured using DPPH, ABTS and FRAP assays) were found to be the highest in the plants grown in full sunlight. However, the plants grown in moderate (30%) shade were found to thrive best.

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Section: Introductionmentioning

confidence: 99%

Effect of Shading on Development, Yield and Quality of Bastard Balm Herb (Melittis melissophyllum L.)

et al. 2020

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“…melissophyllum and subsp. albida differ in chemical composition of the essential oil [ 31 ] and iridoid fraction [ 32 ]. M .…”

Section: Introductionmentioning

confidence: 99%

Enhanced accumulation of harpagide and 8-O-acetyl-harpagide in Melittis melissophyllum L. agitated shoot cultures analyzed by UPLC-MS/MS

Skrzypczak-Pietraszek

Reiss

Żmudzki

et al. 2018

PLoS ONE

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Harpagide and its derivatives have valuable medicinal properties, such as anti-inflammatory, analgesic and potential antirheumatic effects. There is the demand for searching plant species containing these iridoids or developing biotechnological methods to obtain the compounds. The present study investigated the effects of methyl jasmonate (MeJa, 50 μM), ethephon (Eth, 50 μM) and L-phenylalanine (L-Phe, 2.4 g/L of medium), added to previously selected variant of Murashige and Skoog medium (supplemented with plant growth regulators: 6-benzylaminopurine 1.0 mg/L, α-naphthaleneacetic acid 0.5 mg/L, gibberellic acid 0.25 mg/L) on the accumulation of harpagide and 8-O-acetyl-harpagide in Melittis melissophyllum L. agitated shoot cultures. Plant material was harvested 2 and 8 days after the supplementation. Iridoids were quantitatively analyzed by the UPLC-MS/MS method in extracts from the biomass and the culture medium. It was found that all of the variants caused an increase in the accumulation of harpagide. In the biomass harvested after 2 days, the highest harpagide content of 247.3 mg/100 g DW was found for variant F (L-Phe and Eth), and the highest 8-O-acetyl-harpagide content of 138 mg/100 g DW for variant E (L-Phe and MeJa). After 8 days, in some variants, a portion of the metabolites was released into the culture medium. Considering the total amount of the compounds (in the biomass and medium), the highest accumulation of harpagide, amounting to 619 mg/100 g DW, was found in variant F, and the highest amount of 8-O-acetyl-harpagide, of 255.4 mg/100 g DW, was found in variant H (L-Phe, MeJa, Eth) when harvested on the 8th day. These amounts were, respectively, 24.7 and 4.8 times higher than in the control culture, and were, respectively, 15 and 6.7 times higher than in the leaves of the soil-grown plant. The total amount of the two iridoids was highest for variant F (0.78% DW) and variant H (0.68% DW) when harvested on the 8th day. The results indicate that the agitated shoot cultures of M. melissophyllum can be a rich source of harpagide and 8-O-acetyl-harpagide, having a potential practical application. To the best of our knowledge we present for the first time the results of the quantitative UPLC-MS/MS analysis of harpagide and 8-O-acetyl-harpagide in M. melissophyllum shoot cultures and the enhancement of their accumulation by means of medium supplementation with elicitors and precursor.

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Constituents of Melittis melissophyllum subsp. albida

Cited by 2 publications

References 30 publications

Effect of Shading on Development, Yield and Quality of Bastard Balm Herb (Melittis melissophyllum L.)

Effect of Shading on Development, Yield and Quality of Bastard Balm Herb (Melittis melissophyllum L.)

Enhanced accumulation of harpagide and 8-O-acetyl-harpagide in Melittis melissophyllum L. agitated shoot cultures analyzed by UPLC-MS/MS

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