Constituents of neutrophil extracellular traps induce in vitro collagen formation in mare endometrium

Rebordão, Maria Rosa; Amaral, Ana; Łukasik, Karolina; Szóstek‐Mioduchowska, Anna; Pinto‐Bravo, Pedro; Galvão, António; Skarzynski, Dariusz J.; Ferreira‐Dias, Graça

doi:10.1016/j.theriogenology.2018.02.001

Cited by 40 publications

(89 citation statements)

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“…Thus, a discrepancy between COL transcripts and the presence of the collagen fibres (protein) in the mare endometrium might be expected. Pathogenesis of endometrosis might be associated with ageing, but also to a plethora of other factors, such as epigenetics, and neutrophil extracellular traps involvement (Rebordão et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Pathogenesis of endometrosis might be associated with ageing, but also to a plethora of other factors, such as epigenetics, and neutrophil extracellular traps involvement (Rebordão et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Specific primers were designed, as well as the reference gene(Table 1), using the Internet-based program Primer-3(Untergasser et al, 2012) andPrimer Premier software (Premier Biosoft Interpairs). Mitochondrial ribosomal protein L32 (MRPL32) was chosen as the most stable internal control gene(Dheda et al, 2004), among four validated reference genes, as described(Rebordão et al, 2018). Using Power SYBER Green PCR Master Mix (Ref.…”

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confidence: 99%

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Collagens and DNA methyltransferases in mare endometrosis

Alpoim-Moreira

Fernandes

Rebordão

et al. 2019

Reprod Domestic Animals

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Inflammation and fibroproliferative diseases may be modulated by epigenetic changes. Therefore, we suggest that epigenetic mechanisms could be involved in equine endometrosis pathogenesis. DNA methylation is one of the methods to evaluate epigenetics, through the transcription of methyltransferases (DNMT1, DNMT3A, DNMT3B). The correlation between DNMTs and collagen (COL) transcripts was assessed for the different Kenney and Doig's (Current Therapy in Theriogenology. Philadelphia: WB Saunders; 1986) endometrium categories. Endometrial biopsies were randomly collected from cyclic mares. Histological classification (category I, n = 13; II A, n = 17; II B, n = 12; and III, n = 7) and evaluation of COL1A2, COL3A1 and DNMTs transcripts by qPCR, were performed. Data were analysed by one‐way analysis of variance (ANOVA), Kruskal–Wallis test and Pearson correlation. As mares aged, there was an increase in endometrium fibrosis (p < .01), and in DNMT1 mRNA (p < .001). Considering DNMT3B transcripts for each category, there was an increase with fibrosis (p < .05). No changes were observed for DNMT1 and DNMT3A transcripts. However, DNMT3A mRNA levels were the highest in all categories (p < .01). In category I endometrium, a positive correlation was observed for transcripts of all DNMTs in both COLs (p < .01). In category IIA, this correlation was also maintained for all DNMTs transcripts in COL1A2 (p < .05), but only for DNMT3B in COL3A1 (p < .05). In category IIB, there was a positive correlation between DNMT3B and COL3A1 (p < .05). In category III, a positive correlation was only observed between DNMT3B and COL3A1 (p < .05). Our results suggest that there is a disturbance in COLs and DNMTs correlation during fibrosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Collagens and DNA methyltransferases in mare endometrosis

Alpoim-Moreira

Fernandes

Rebordão

et al. 2019

Reprod Domestic Animals

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“…The initiating event as well as details about the pathogenesis of equine endometrosis are the focus of ongoing research [67,68].…”

Section: The Pathogenesis Of Endometrosis-still To Be Revealedmentioning

confidence: 99%

“…Neutrophils attracted by bacterial infections release DNA components that lead to the formation of neutrophilic extracellular traps (NETs) [19,67,69]. In vitro studies show that the NETs components myeloperoxidase, elastase, and cathepsin G increase transcript levels of collagen type I and collagen type III as well as of factors that promote fibrosis, i.e., tumor growth factor β1 and tissue inhibitor of metalloproteinases depending on the stage of the estrous cycle and preexisting category of the endometrial tissue sample [19,67]. In addition, they decrease prostaglandin E2 that exerts an antifibrotic response after binding to the E prostanoid receptor [19].…”

Section: The Pathogenesis Of Endometrosis-still To Be Revealedmentioning

confidence: 99%

The Healthy and Diseased Equine Endometrium: A Review of Morphological Features and Molecular Analyses

Schöniger¹,

Schoon

2020

Animals

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Mares are seasonally polyestric. The breeding season in spring and summer and the winter anestrus are flanked by transitional periods. Endometrial diseases are a frequent cause of subfertility and have an economic impact on the horse breeding industry. They include different forms of endometrosis, endometritis, glandular maldifferentiation, and angiosis. Except for suppurative endometritis, these are subclinical and can only be diagnosed by the microscopic examination of an endometrial biopsy. Endometrosis is characterized by periglandular fibrosis and nonsuppurative endometritis by stromal infiltration with lymphocytes and plasma cells. The pathogenesis of endometrosis and nonsuppurative endometritis is still undetermined. Some mares are predisposed to persistent endometritis; this has likely a multifactorial etiology. Glandular differentiation has to be interpreted under consideration of the season. The presence of endometrial diseases is associated with alterations in the expression of several intra- and extracellular molecular markers. Some of them may have potential to be used as diagnostic biomarkers for equine endometrial health and disease. The aim of this review is to provide an overview on pathomorphological findings of equine endometrial diseases, to outline data on analyses of cellular and molecular mechanisms, and to discuss the impact of these data on reproduction and treatment.

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