Constitutive expression of DaCBF7, an Antarctic vascular plant Deschampsia antarctica CBF homolog, resulted in improved cold tolerance in transgenic rice plants

Byun, Mi Young; Lee, Jung-Eun; Cui, Li; Kang, Yoonjee; Oh, Tae Kyung; Park, Hyun; Lee, Hyoungseok; Kim, Woo Taek

doi:10.1016/j.plantsci.2015.03.020

Cited by 78 publications

(55 citation statements)

References 69 publications

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“…D . antarctica might also be a useful source of genes associated with stress tolerance and environmental adaptation, and its karyotype can be a basic tool for crop breeding strategies in agronomical valuable crops [17–19]. Besides, extracts from D .…”

Section: Introductionmentioning

confidence: 99%

Comparative molecular cytogenetic characterization of seven Deschampsia (Poaceae) species

et al. 2017

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The genus Deschampsia P. Beauv (Poaceae) involves a group of widespread polymorphic species. Some of them are highly tolerant to stressful and variable environmental conditions, and D. antarctica is one of the only two vascular plants growing in Antarctic. This species is a source of useful for selection traits and a valuable model for studying an environmental stress tolerance in plants. Genome diversity and comparative chromosomal phylogeny within the genus have not been studied yet as karyotypes of most Deschampsia species are poorly investigated. We firstly conducted a comparative molecular cytogenetic analysis of D. antarctica (Antarctic Peninsula) and related species from various localities (D. cespitosa, D. danthonioides, D. elongata, D. flexuosa (= Avenella flexuosa), D. parvula and D. sukatschewii by fluorescence in situ hybridization with 45S and 5S rDNA, DAPI-banding and sequential rapid in situ hybridization with genomic DNA of D. antarctica, D. cespitosa, and D. flexuosa. Based on patterns of distribution of the examined markers, chromosomes of the studied species were identified. Within these species, common features as well as species peculiarities in their karyotypic structure and chromosomal distribution of molecular cytogenetic markers were characterized. Different chromosomal rearrangements were detected in D. antarctica, D. flexuosa, D. elongata and D. sukatschewii. In karyotypes of D. antarctica, D. cespitosa, D. elongata and D. sukatschewii, 0–3 B chromosomes possessed distinct DAPI-bands were observed. Our findings suggest that the genome evolution of the genus Deschampsia involved polyploidy and also different chromosomal rearrangements. The obtained results will help clarify the relationships within the genus Deschampsia, and can be a basis for the further genetic and biotechnological studies as well as for selection of plants tolerant to extreme habitats.

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Section: Introductionmentioning

confidence: 99%

Comparative molecular cytogenetic characterization of seven Deschampsia (Poaceae) species

et al. 2017

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“…Ultraviolet-B treated D. antarctica showed a discrete but significant decrease in F v/ F m, revealing a partial stress (a decrease of 5% relative to the control plants), and it recuperated to normal levels after 3 h in the dark, supporting the efficiency of stress responsive elements (Byun et al, 2015). This is probably because this particular Antarctic plant has developed several mechanisms to dissipate excess energy reaching chloroplasts (Ruhland and Day, 2000; Frohnmeyer and Staiger, 2003; Ruhland et al, 2005) Effects.…”

Section: Discussionmentioning

confidence: 75%

Antioxidant Responses Induced by UVB Radiation in Deschampsia antarctica Desv.

et al. 2017

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Deschampsia antarctica Desv. is one of two vascular plants that live in the Maritime Antarctic Territory and is exposed to high levels of ultraviolet-B (UVB) radiation. In this work, antioxidant physiology of D. antarctica was studied in response to UVB induced oxidative changes. Samples were collected from Antarctica and maintained in vitro culture during 2 years. Plants were sub-cultured in a hydroponic system and exposed to 21.4 kJ m −2 day −1 , emulating summer Antarctic conditions. Results showed rapid and significant increases in reactive oxygen species (ROS) at 3 h, which rapidly decreased. No dramatic changes were observed in photosynthetic efficiency, chlorophyll content, and level of thiobarbituric acid reactive species (MDA). The enzymatic (superoxide dismutase, SOD and total peroxidases, POD) and non-enzymatic antioxidant activity (total phenolic) increased significantly in response to UVB treatment. These findings suggest that tolerance of D. antarctica to UVB radiation could be attributed to its ability to activate both enzymatic and non-enzymatic antioxidant systems.

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“…The 35S:OsPUB2-sGFP, 35S:OsPUB2-mRFP, 35S:OsPUB3-sGFP, 35S:OsPUB3-mRFP, 35S:NLS-mRFP , and 35S:AtExo70E2-sGFP constructs were expressed in tobacco ( Nicotiana benthamiana ) leaves by using Agrobacterium- mediated method as described in a previous study (Kim and Kim, 2013). Two days after infection, protoplasts were extracted from the tobacco leaves, and fluorescent protein signals were visualized by fluorescence microscopy (BX51, Olympus, Japan) as described by Byun et al (2015). NLS-mRFP and AtExo70E2-sGFP were used as nucleus and EXPO marker proteins, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…For cold stress treatment, 5-week-old wild-type and transgenic rice plants were transferred to cold room at 4°C for 6 days, after which plants were recovered at 28°C and their growth patterns were monitored as described previously (Byun et al, 2015). Total chlorophyll (chlorophyll a + chlorophyll b) was extracted from wild-type and transgenic leaves before and after cold treatment according to Lichtenthaler (1987) with modifications as described by Min et al (2016).…”

Section: Methodsmentioning

confidence: 99%

Homologous U-box E3 Ubiquitin Ligases OsPUB2 and OsPUB3 Are Involved in the Positive Regulation of Low Temperature Stress Response in Rice (Oryza sativa L.)

Byun

Cui

et al. 2017

Front. Plant Sci.

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Rice U-box E3 Ub ligases (OsPUBs) are implicated in biotic stress responses. However, their cellular roles in response to abiotic stress are poorly understood. In this study, we performed functional analyses of two homologous OsPUB2 and OsPUB3 in response to cold stress (4°C). OsPUB2 was up-regulated by high salinity, drought, and cold, whereas OsPUB3 was constitutively expressed. A subcellular localization assay revealed that OsPUB2 and OsPUB3 were localized to the exocyst positive organelle (EXPO)-like punctate structures. OsPUB2 was also localized to the nuclei. OsPUB2 and OsPUB3 formed a hetero-dimeric complex as well as homo-dimers in yeast cells and in vitro. OsPUB2/OsPUB3 exhibited self-ubiquitination activities in vitro and were rapidly degraded in the cell-free extracts with apparent half-lives of 150–160 min. This rapid degradation of OsPUB2/OsPUB3 was delayed in the presence of the crude extracts of cold-treated seedlings (apparent half-lives of 200–280 min). Moreover, a hetero-dimeric form of OsPUB2/OsPUB3 was more stable than the homo-dimers. These results suggested that OsPUB2 and OsPUB3 function coordinately in response to cold stress. OsPUB2- and OsPUB3-overexpressing transgenic rice plants showed markedly better tolerance to cold stress than did the wild-type plants in terms of survival rates, chlorophyll content, ion leakage, and expression levels of cold stress-inducible marker genes. Taken together, these results suggested that the two homologous rice U-box E3 Ub ligases OsPUB2 and OsPUB3 are positive regulators of the response to cold stress.

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Constitutive expression of DaCBF7, an Antarctic vascular plant Deschampsia antarctica CBF homolog, resulted in improved cold tolerance in transgenic rice plants

Cited by 78 publications

References 69 publications

Comparative molecular cytogenetic characterization of seven Deschampsia (Poaceae) species

Comparative molecular cytogenetic characterization of seven Deschampsia (Poaceae) species

Antioxidant Responses Induced by UVB Radiation in Deschampsia antarctica Desv.

Homologous U-box E3 Ubiquitin Ligases OsPUB2 and OsPUB3 Are Involved in the Positive Regulation of Low Temperature Stress Response in Rice (Oryza sativa L.)

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