Constitutive transcription of a soybean heat‐shock gene by a cauliflower mosaic virus promoter in transgenic tobacco plants

Schöffl, Fritz; Rieping, Mechthild; Baumann, Götz

doi:10.1002/dvg.1020080507

Cited by 14 publications

(6 citation statements)

References 37 publications

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“…1994 ). Similarly, Schoffl et al . (1987 , 1992) reported differences in small Hsp mRNA levels, utilizing antisense and over‐expression constructs in tobacco, but did not address the effects of these constructs on thermotolerance.…”

Section: Introductionmentioning

confidence: 65%

“…In Drosophila, Hsp 70 mRNA was shown to be extremely unstable when transcribed at 23°C under the control of the metallothionine promoter, but was stabilized when the temperature increased ( Petersen & Lindquist 1988<!hide{Q1} Petersen & Lindquist 1988 has been changed to Petersen & Lindquist 1988 so that this citation matches the list>). However, Schoffl et al . (1987) reported high accumulation of a soybean Hsp mRNA in transgenic tobacco cells at room temperature when the heat shock gene was driven by the 35S‐promoter.…”

Section: Discussionmentioning

confidence: 99%

“…This self‐recognition may play an important role in the instability of the mRNA at 23°C. It is interesting to note that although the tobacco cells accumulated high levels of soybean Hsp mRNA, there was no indication that these tobacco cells were more thermotolerant ( Schoffl et al . 1987 ).…”

Section: Discussionmentioning

confidence: 99%

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Modified expression of a carrot small heat shock protein gene, Hsp17.7, results in increased or decreased thermotolerance^‡

Slovin²,

et al. 1999

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Summary We have determined that one small heat shock protein gene, encoding Hsp17.7, plays an important role in the ability of carrot cells and plants to survive thermal stress. Transgenic cells and regenerated plants were generated in which the carrot Hsp17.7 gene was either constitutively expressed (denoted CaS lines) or expressed as a heat inducible antisense RNA (denoted AH lines). Thermotolerance measurements demonstrated that CaS lines were more thermotolerant than vector controls and AH antisense lines were less thermo‐ tolerant than vector controls. RNA analysis demonstrated that Hsp17.7 mRNA was detectable, but not abundant, prior to heat shock in CaS cells, but not in vector control cells. Conversely, RNA analysis of antisense cells showed that, after heat shock, the amounts of mRNA for Hsp17.7 was moderately less abundant in AH cells than in vector controls. Analysis of protein synthesis in CaS cells did not indicate substantial synthesis or accumulation of Hsp17.7, or any small Hsp, at 23°C. However, in the most thermotolerant line, protein synthesis was maintained at a higher rate than in other cell lines at a more extreme heat shock (42°C). In contrast, antisense AH cells showed reduced synthesis of many Hsp, large and small. These results suggest that the Hsp17.7 gene plays a critical, although as yet not understood, role in thermotolerance in carrot. This represents the first demonstration of the ability to both increase and decrease thermotolerance by the manipulation of expression of a single gene.

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Section: Introductionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

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Modified expression of a carrot small heat shock protein gene, Hsp17.7, results in increased or decreased thermotolerance^‡

Slovin²,

et al. 1999

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“…Several attempts to modify the cell content of HSPs via genetic engineering have been made. Transgenic tobacco expressed introduced HSP17.6 constitutively (Schoffl et al, 1987); however, due to the incompetence of the constitutive promoter, expression of the transgene was inhibited at high temperature and no conclusion was made concerning its effect on plant thermotolerance. No effect of overexpression of chloroplast-localized HSP21 on thermotolerance was observed in transgenic Arabidopsis plants (Osteryoung et al, 1994), possibly due to the overly high level of HSP21 accumulation.…”

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confidence: 96%

Genetic engineering for abiotic stress resistance in crop plants

Zhang

Klueva

Wang

et al. 2000

In Vitro Cell.Dev.Biol.-Plant

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Drought, extreme temperatures and high salinity are major limiting factors for plant growth and crop productivity. In their quest to feed the ever-increasing world population, agricultural scientists have to contend with these adverse environmental factors. If crops can be redesigned to better cope with abiotic stress, agricultural production can be increased dramatically. Recent advances in understanding crop abiotic stress resistance mechanisms and the advent of molecular genetic technology allow us to address these issues much more efficiently than in the past. This paper reviews the most significant achievements of the genetic engineering approach to improving plant abiotic stress resistance and discusses future prospects in transgenic research. Improved resistance to drought, salinity and extreme temperatures has been observed in transgenic plants that express/overexpress genes regulating osmolytes, specific proteins, antioxidants, ion homeostasis, transcription factors and membrane composition. Although the results are not always consistent, these studies collectively foretell a scenario where biotechnology will arm our future crops with new tactics to survive in hostile environments. Further experiments are needed to determine if the achieved increases in stress tolerance are applicable to agriculture.

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“…Although the overexpressions of Hsp70 from Drosophila and mammalian conferred the thermotolerance of their organisms (Solomon et al 1991;Kim et al 1995), the overexpression of small Hsp from soybean was not sufficient to confer the thermotolerance in transgenic tobacco plants (Schoffl et al 1987). Later, the transgenic plants constitutively expressing the heat shock factor (HSF) of HSP was shown to confer the thermotolerance (Lee et al 1995).…”

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confidence: 99%

Overexpression of DnaK chaperone from a halotolerant cyanobacterium Aphanothece halophytica increases seed yield in rice and tobacco

Uchida

Hibino

Shimada³

et al. 2008

Plant Biotechnology

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The DnaK/Hsp70 family is a molecular chaperone that binds non-native states of other proteins, and concerns to various physiological processes in the bacterial, plant and animal cells. Transgenic tobacco plants expressing a molecular chaperone DnaK from a halotolerant cyanobacterium Aphanothece halophytica show enhanced seed yields as well as enhanced tolerances for salt and heat stresses. High-temperature treatment during the reproductive stage decreased total dry weight of seeds in both transgenic and wild-type tobacco, but more severely in the wild-type. Transgenic tobacco plants exhibited higher activities of ascorbate peroxidase and catalase than wild-type plants. Similar results were obtained for salt stress during the reproductive stage. Transgenic rice plants expressing ApDnaK was also constructed. Transgenic rice plants exhibited the enhanced activities for Calvin-cycle enzymes, and showed faster growth and higher seed yield compared with the wild-type rice under normal growth conditions. Transgenic rice plants also showed enhanced tolerance for high temperature and salt stress compared with the wild-type rice. These results suggest a relation of increased folding activity with enhanced stress tolerance, increased seed yield, and total plant biomass. Key words:Aphanothece halophytica, chaperone, DnaK, high temperature stress, rice, salt tolerance, seed yield.Plant Biotechnology 25, 141-150 (2008) Original PaperAbbreviations: Ap, Aphanothece halophytica; CaMV, cauliflower mosaic virus; HSF, heat shock factor; Hsp, heat shock proteins; MS, Murashige and Skoog; PRK, phosphoribulokinase; FBPase, cytosolic fructose-1,6-bisphosphatase; SPS, sucrose phosphate synthetase. This article can be found at http://www.jspcmb.jp/ (HSF) of HSP was shown to confer the thermotolerance (Lee et al. 1995). The overexpressions of carrot small Hsp (Malik et al. 1999) and Arabidopsis Hsp101 (Queitsch et al. 2000;Hong and Vierling 2001) have shown to enhance the thermotolerance of their own cells. The overexpression of Arabidopsis small Hsp was shown to enhance the osmotolerance of Arabidopsis (Sun et al. 2001). We also showed that the overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica (ApDnaK) which was isolated from The Dead Sea, conferred the tolerances for salt (Sugino et al. 1999) and high temperature (Ono et al. 2001) in transgenic tobacco plants. However, all these studies did not show the effects of overexpression of HSP on crop yield as well as growth promotion. Crop yield is one of the most important factors for agriculture. ApDnaK was previously shown to exhibit extremely high protein folding activity even under high salinity conditions . Here, we report that the transgenic rice and tobacco plants expressing ApDnaK could increase the seed yield and plant biomass in the laboratory conditions. Materials and methods Construction of vector and transformationTobacco plant (Nicotiana tabacum cv. Petit Havana SR1) was transformed with the dnaK gene from A. halophytica as described ...

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Constitutive transcription of a soybean heat‐shock gene by a cauliflower mosaic virus promoter in transgenic tobacco plants

Cited by 14 publications

References 37 publications

Modified expression of a carrot small heat shock protein gene, Hsp17.7, results in increased or decreased thermotolerance^‡

Modified expression of a carrot small heat shock protein gene, Hsp17.7, results in increased or decreased thermotolerance^‡

Genetic engineering for abiotic stress resistance in crop plants

Overexpression of DnaK chaperone from a halotolerant cyanobacterium Aphanothece halophytica increases seed yield in rice and tobacco

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Constitutive transcription of a soybean heat‐shock gene by a cauliflower mosaic virus promoter in transgenic tobacco plants

Cited by 14 publications

References 37 publications

Modified expression of a carrot small heat shock protein gene, Hsp17.7, results in increased or decreased thermotolerance‡

Modified expression of a carrot small heat shock protein gene, Hsp17.7, results in increased or decreased thermotolerance‡

Genetic engineering for abiotic stress resistance in crop plants

Overexpression of DnaK chaperone from a halotolerant cyanobacterium Aphanothece halophytica increases seed yield in rice and tobacco

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Modified expression of a carrot small heat shock protein gene, Hsp17.7, results in increased or decreased thermotolerance^‡

Modified expression of a carrot small heat shock protein gene, Hsp17.7, results in increased or decreased thermotolerance^‡