13A multilayer solar planted floating island (MS-PFI) planted with Eichhornia 14 crassipes are potential alternatives to traditional PFI. The highest removal rates of 15 suspended solids, total nitrogen, total phosphorus, ammonia nitrogen and chemical 16 oxygen demand was 86%, 75%, 80%, 95% and 84%, respectively. Proteobacteria 17 (average 43.4% of total sequences) and Actinobacteria (19.9%) were the dominant 18 phyla. Numerous genus had obvious differences between influent and effluent water, for 19 instance, 13, 12 and 7 % in effluent water were assigned to the hgcl_clade, 20Norank_c_Cyanobacteria, and Rhizorhapis, while their relative abundances were 21 decreased to 5, 3 and 0 %. In contrast, a distinct increase among Flavobacterium (10%), 22Limnohabitans (7%), Alpinimonas (4%), norank_p_Saccharibacteria (4%), Erwinia (3%) 23 after MS-PFI treatment. MS-PFI brings various bacteria involved in contaminant 24 degradation and nutrient removal in biological wastewater treatment systems. An 25 amount of ¥ 1,843 was totally inputted to construct floating bed, which was rarely 26 needed operation and maintenance costs. 27Importance 28In-situ micro-polluted water ecological remediation, microorganisms and plants are 29 effective to improve environmental quality and provide essential ecosystem services. 30Recently, we invent a new multilayer solar with an excellent pollutant removal 31 efficiency. Microbes can decompose or mineralize organic matter effectively, also 32All rights reserved. No reuse allowed without permission.(which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint . http://dx.doi.org/10.1101/327965 doi: bioRxiv preprint first posted online May. 23, 2018; provide food for aquatic animals and increase nutrients or substances for plants, it is an 33 important part of biogeochemical cycles and energy flows in aquatic ecological systems. 34However, few study explain the bacteria diversity and its responses between influent 35 and effluent water in a planted floating island. The significance of our study is in 36 identifying-in greater detail-the responses of bacteria in the new MS-PFI. This will 37 greatly enhance our knowledge of bacteria communities, and can be widely used in 38 micro-polluted water remediation. 39