Constructing a continuous hemodiafiltration‐type circulatory model of acute kidney injury in pigs

Abstract

Introduction Animal‐model experimental systems capable of reflecting the effects of devices for continuous renal replacement therapy (CRRT) on living organisms are limited; thus, aimed to construct an animal model of AKI‐CRRT using pigs. Methods Pigs were subjected to renal artery ischemia–reperfusion injury (IRI) and then to a maximum of 24 h of continuous hemodiafiltration (CHDF)‐type CRRT. Results Post‐IRI, pigs' creatinine levels rose threefold, and they exhibited 24 h of anuria and clear aggravation of oxidative stress, demonstrating successful induction of AKI for CRRT. Post‐CRRT, no significant changes in their vital signs or hematological parameters were observed. Creatinine and blood urea nitrogen clearance, as well as suppression of increases in oxidative stress, were also confirmed. Conclusion We believe that the use of our model can enable the preclinical evaluation of the effects of under‐development CRRT devices on living organisms under conditions similar to those encountered in an actual clinical setting.

Biomarker array for prediction of acute kidney injury after percutaneous coronary intervention for patients who had acute ST segment elevation myocardial infarction

“…The development of AKI at 12 h after revascularization (T12 time) may be secondary to the effect of the washed inflammatory cytokines and free radicals in association with cardiac and renal ischemia–reperfusion injury as previously detected in animal models of ischemia–reperfusion AKI [ 28 , 29 ] or may be due to early-onset contrast-induced AKI (CI-AKI) that was previously detected using an animal model of CI-AKI where microscopic examination assured that the strongest renal damage in CI-AKI existed between 12 and 24 h after CM injection [ 28 ]. On the contrary, AKI cases detected at 72 h after revascularization (T72 time) are considered CI-AKI as previously documented [ 4 ].…”