Constructing Biopolymer-Inorganic Nanocomposite through a Biomimetic Mineralization Process for Enzyme Immobilization

Abstract

Inspired by biosilicification, biomimetic polymer-silica nanocomposite has aroused a lot of interest from the viewpoints of both scientific research and technological applications. In this study, a novel dual functional polymer, NH2-Alginate, is synthesized through an oxidation-amination-reduction process. The “catalysis function” ensures the as-prepared NH2-Alginate inducing biomimetic mineralization of silica from low concentration precursor (Na2SiO3), and the “template function” cause microscopic phase separation in aqueous solution. The diameter of resultant NH2-Alginate micelles in aqueous solution distributed from 100 nm to 1.5 μm, and is influenced by the synthetic process of NH2-Alginate. The size and morphology of obtained NH2-Alginate/silica nanocomposite are correlated with the micelles. NH2-Alginate/silica nanocomposite was subsequently utilized to immobilize β-Glucuronidase (GUS). The harsh condition tolerance and long-term storage stability of the immobilized GUS are notably improved due to the buffering effect of NH2-Alginate and cage effect of silica matrix.

Hybrid mesoporous microspheres from aqueous droplets containing a silica nanoparticle–polymer network in a W/O suspension

“…For all the microspheres, the higher Q 4 values obtained aer pyrolysis inferred an increase of the interlinked SiO 4 tetrahedrons in the interior of the microspheres. [41][42][43][44] Surface area and pore size distribution analysis…”

Direct Exposure of Dry Enzymes to Atmospheric Pressure Non-Equilibrium Plasmas: The Case of Tyrosinase

“…Notwithstanding this, generalizable immobilization procedures are far from being assessed, because the widely different structural and functional characteristics of enzymes do not allow identifying a single procedure and solid support suitable for all enzymes and their diverse applications [5]. Moreover, each immobilization method has its own advantages and disadvantages [5][6][7][8][9][10][11][12][13][14][15][16]. Adsorption is simple, cheap, and effective, but very often reversible; covalent bonding effectively improves durability, but is expensive and easily worsens enzyme performance; encapsulation (or confinement) suffers from problems associated with mass-transfer limitations.…”

Polymerization-Driven Immobilization of dc-APGD Synthesized Gold Nanoparticles into a Quaternary Ammonium-Based Hydrogel Resulting in a Polymeric Nanocomposite with Heat-Transfer Applications

“…Polymeric nanocomposites are a unique type of NMs that can consist of metallic NPs dispersed in a polymeric phase. These materials can be used as biosensors [ 25 ], optical devices [ 26 ], or a platform for the immobilization of proteins [ 27 ]. The utility of nanocomposites is associated with the type of the polymeric matrix as well as the nanostructures dispersed within it.…”