Ecological flow refers to the minimum amount of water that must be maintained in a body of water to protect and preserve aquatic ecosystems. This article aims to analyze the function of ecological flow to address the reproduction of the natural hydrological regime of the San Rodrigo River, Coahuila, Mexico. A quantitative methodology was used where the ecological flow was determined based on the Mexican Standard NMX-AA-159-SCFI-2012 considering the period from 1962 to 2016. The maximum data allows us to identify runoffs of low magnitude of 6.65–15.60, those with an average trend close to 500 Mm3, and floods of extraordinary volume (namely, those 844–1260 and 1670 Mm3) with a frequency of every 35 years. Likewise, the river marks drastic changes in the flow in certain years, ranging from 0.64 to 1260 Mm3, so that the rate of variation would possibly exceed several orders of magnitude scaled in an annual phase. In conclusion, this calculation indicates that the body of water may have the function of environmental conservation covered throughout the year, with the data suggesting that in the short term the river will recover part of the water that passed through its course and thus avoid its deterioration.