Construction and Evaluation of Osteochondral-Like Tissue Using Chondrocyte Sheet and Cancellous Bone

Wongin, Sopita; Narkbunnam, Rapeepat; Waikakul, Saranatra; Chotiyarnwong, Pojchong; Aresanasuwan, Thanyawan; Roytrakul, Sittiruk; Viravaidya-Pasuwat, Kwanchanok

doi:10.1089/ten.tea.2020.0107

Cited by 10 publications

(6 citation statements)

References 44 publications

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“…In the human umbilical cord tissue, among the above ligand–receptor pairs, adhesion receptor ITGB6 (integrin subunit beta 6) was highly expressed in epithelial‐like cells (Fig. 6B) and communicated with other cell groups by binding to ligand FN1 (fibronectin 1) [29]; in addition, PLAU‐PLAUR was identified to augment tissue remodeling by promoting epithelial‐to‐mesenchymal transition [30], which was in line with our observations (Fig. 6B).…”

Section: Resultsmentioning

confidence: 99%

Single‐cell transcriptome profiling reveals molecular heterogeneity in human umbilical cord tissue and culture‐expanded mesenchymal stem cells

Wang

et al. 2021

The FEBS Journal

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Human umbilical cord‐derived mesenchymal stem/stromal cells (UMSCs) demonstrate great therapeutic potential in regenerative medicine. The use of UMSCs for clinical applications requires high quantity and good quality of cells usually by in vitro expansion. However, the heterogeneity and the characteristics of cultured UMSCs and the cognate human umbilical cord tissue at single‐cell resolution remain poorly defined. In this study, we created a single‐cell transcriptome profile of human umbilical cord tissue and the cognate culture‐expanded UMSCs. Based on the inferred characteristics of cell clusters and trajectory analysis, we identified three subgroups in culture‐expanded UMSCs and putative novel transcription factors (TFs) in regulating UMSC state transition. Further, putative ligand–receptor interaction analysis demonstrated that cellular interactions most frequently occurred in epithelial‐like cells with other cell groups in umbilical cord tissue. Moreover, we dissected the transcriptomic differences of in vitro and in vivo subgroups and inferred the telomere‐related molecules and pathways that might be activated in UMSCs for cell expansion in vitro. Our study provides a comprehensive and integrative study of the transcriptomics of human umbilical cord tissue and their cognate‐cultured counterparts, which paves the way for a deeper understanding of cellular heterogeneity and offers fundamental biological insight of UMSCs‐based cell therapy.

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Section: Resultsmentioning

confidence: 99%

Single‐cell transcriptome profiling reveals molecular heterogeneity in human umbilical cord tissue and culture‐expanded mesenchymal stem cells

Wang

et al. 2021

The FEBS Journal

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“…ITGB4 has been reported to be involved in multiple biological processes, such as cell proliferation, migration, and apoptosis (Li et al, 2019). Sopita et al demonstrated that ITGB4 was expressed in chondrocyte sheets (Wongin et al, 2020). ITGB4 also regulates ECM that exerts a crucial role in the pathogenesis of OA (Zhong et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Valproic Acid Protects Chondrocytes from LPS-Stimulated Damage via Regulating miR-302d-3p/ITGB4 Axis and Mediating the PI3K-AKT Signaling Pathway

Sun

Zheng

Liu

et al. 2021

Front. Mol. Biosci.

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Background: Osteoarthritis (OA) is one of the most common degenerative joint diseases characterized by increased apoptosis and autophagy deficiency. The investigation was performed to examine the effect of valproic acid (VPA) and molecular mechanism related to miR-302d-3p/ITGB4 axis in OA.Methods: The OA clinical samples were obtained from the GEO database to analyze differentially expressed genes. An in vitro OA model was mimicked by LPS in CHON-001 cells. Autophagy-related genes were downloaded from the HADb website, and potential drugs were mined using the CTD website. The upstream factors of ITGB4 were predicted with bioinformatics analysis, which was validated by luciferase activity assay and RIP assay. Cell viability and apoptosis were evaluated using CCK-8 and flow cytometry. The expression levels, including ITGB4, miR-302d-3p, and autophagy-/PI3K-AKT pathway-related markers, were measured by qRT-PCR or/and western blot.Results: Our results showed that miR-302d-3p inhibited cell viability and promoted apoptosis of LPS-treated CHON-001 cells by targeting ITGB4. VPA treatment remarkably alleviated LPS-stimulated injury in CHON-001 cells. The inhibitory effect of VPA on LPS-stimulated damage in CHON-001 cells was weakened by miR-302d-3p overexpression, while it was intensified because of ITGB4 upregulation. Mechanistically, VPA treatment induced a significant decrease in the levels of p-PI3K and p-AKT in LPS-stimulated CHON-001 cells through regulating miR-302d-3p/ITGB4 axis.Conclusion: Overall, VPA treatment may ameliorate LPS-induced injury on chondrocytes via the regulation of miR-302d-3p/ITGB4 pair and the inactivation of the PI3K-AKT pathway.

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“…On the other hand, knocking down ITGB8 markedly impacted MSCs' chondrogenic differentiation [67]. A recent publication also indicates that ITGB8 plays a key role in chondrocyte sheet attachment and the in ECM synthesis in cartilage [68]. In combination, ITGB8 seems to have a beneficial effect on cartilage.…”

Section: Discussionmentioning

confidence: 99%

“…Since cartilage has no nerve intervention [31], the OA-responsive adaption of gene expression is more than likely from chondrocytes. Meanwhile, accumulating studies demonstrate that the crosstalk between skeletal and neural tissue is critical for skeletal health [68,69]. For example, there is bidirectional communication between the sensory neurons and osteoblasts through glutamate, substance P, and ATP [70].…”

Section: Discussionmentioning

confidence: 99%

Cartilage Targets of Knee Osteoarthritis Shared by Both Genders

Zheng

2021

IJMS

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As the leading cause of disability, osteoarthritis (OA) affects people of all ages, sexes, and races. With the increasing understanding of OA, the sex differences have attracted specific attention as the burden of OA is greater in women. There is no doubt that gender-specific OA management has great potential for precision treatment. On the other hand, from the marketing aspect, a medication targeting the OA-responsive biomarker(s) shared by both genders is more favorable for drug development. Thus, in the current study, a published transcriptome dataset of knee articular cartilage was used to compare OA and healthy samples for identifying the genes with the same significantly different expression trend in both males and females. With 128 genes upregulated and 143 genes downregulated in both OA males and females, 9 KEGG pathways have been enriched based on the current knowledge, including ‘renal cell carcinoma,’ ‘ECM-receptor interaction,’ ‘HIF-1 signaling pathway,’ ‘MicroRNAs in cancer,’ ‘focal adhesion,’ ‘Relaxin signaling pathway,’ ‘breast cancer,’ ‘PI3K-Akt signaling pathway,’ and ‘human papillomavirus infection.’ Here, we explore the potential impacts of these clusters in OA. We also analyze the identified ‘cell plasma membrane related genes’ in-depth to identify the potential chondrocyte cell surface target(s) of OA management.

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Construction and Evaluation of Osteochondral-Like Tissue Using Chondrocyte Sheet and Cancellous Bone

Cited by 10 publications

References 44 publications

Single‐cell transcriptome profiling reveals molecular heterogeneity in human umbilical cord tissue and culture‐expanded mesenchymal stem cells

Single‐cell transcriptome profiling reveals molecular heterogeneity in human umbilical cord tissue and culture‐expanded mesenchymal stem cells

Valproic Acid Protects Chondrocytes from LPS-Stimulated Damage via Regulating miR-302d-3p/ITGB4 Axis and Mediating the PI3K-AKT Signaling Pathway

Cartilage Targets of Knee Osteoarthritis Shared by Both Genders

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