Construction and fed-batch cultivation of Candida famata with enhanced riboflavin production

Endogenous superoxide dismutase activation by oral administration of riboflavin reduces abdominal aortic aneurysm formation in rats

“…The main role of riboflavin is to act as a precursor for coenzymes. 29 In addition, riboflavin works as an antioxidant by affecting the levels of antioxidant enzymes. 30 In this study, SOD activity was increased by the administration of riboflavin in the treatment group.…”

Production of riboflavin and related cofactors by biotechnological processes

“…In A. gossypii, overexpression of the RIB genes also significantly promoted the yield of RF [53]. In C. famata, overexpression of the genes RIB1 and RIB7, respectively encoding GTP cyclohydrolase II and riboflavin synthase, led to increased production of RF [30]. In E. coli, overexpression of an artificial RF operon called EC10 (Ptrc-ribABDEC) was used to successfully engineer RF overproduction [54].…”

“…In C. ammoniagenes, a plasmid containing the native RF biosynthetic genes led to 17-fold higher RF accumulation compared to the host strain [88]. In C. famata, overexpression of the RIB1 and RIB7 genes encoding GTP cyclohydrolase II and RF synthase led to a significant increase of the RF titer [30]. The RIB1 and RIB3 genes from A. gossypii, which respectively encode GTP-cyclohydrolase II (GCH II) and DHPB synthase were overexpressed in E. ashbyi to achieve RF overproduction [89].…”

“…The industrial strains used for RF production mainly are mainly derived from the bacterium Bacillus subtilis (B. subtilis) and the fungus Ashbya gossypii (A. gossypii) [27]. The yeast Candida famata (C. famata) was once also used for the industrial production of RF [28], but the ADM Company (USA) has stopped this industrial process because it was unprofitable due to the low stability of the strain [1,29,30]. As the engineered RF production strains are typically acquired by a combination of mutagenesis and genetic engineering, the genetic background is often complex and can be perplexing.…”

Expression of yeast homolog of the mammal BCRP gene coding for riboflavin efflux protein activates vitamin B2 production in the flavinogenic yeast Candida famata

“…of the RIB1 and RIB7 genes, which encode the first and the last structural enzymes of riboflavin synthesis, GTP-cyclohydrolase II and riboflavin synthase, respectively in background of stable riboflavin overproducer AF-4 isolated by multistep selection for resistance to antimetabolites (Dmytruk et al, 2014;Dmytruk, Yatsyshyn, Sybirna, Fedorovych, & Sibirny, 2011). Despite significant increase of riboflavin accumulation, further strain improvement is needed to compete with current industrial producers B. subtilis and A. gossypii.…”