Healthy buildings are the future of industrial development and a global trend. This study is based on the local demand in Taiwan for the certification of healthy building assessments. It consolidates fifteen relevant assessment indicators and the literature on healthy buildings and green buildings from both domestic and international sources. Through expert questionnaires, the study investigates the importance and weight values of assessment items, selecting seven assessment indicators (air, water, light, exercise, comfort, materials, and mental well-being), seventeen assessment items, and 65 assessment sub-items. The weight values of each indicator are statistically analyzed. Based on the expert questionnaires, a rating system and scoring criteria are formulated, ultimately constructing the “Taiwan Healthy Building Assessment Indicators.” The aim is for this framework to serve as a reference for the government in establishing a healthy building certification system as well as to enhance public awareness and emphasis on human health.