Construction of a Bioreactor with Immobilized Yeast Cells for Production of a Low-phenylalanine Peptide Mixture as a Phenylketonuria Foodstuff

Watanabe, Michiko; Matsumura, Masahiko; Yabuki, Soichi; Aizawa, Masuo; Arai, Soichi

doi:10.1080/00021369.1988.10869171

Cited by 3 publications

(3 citation statements)

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“…This contrasts to the design of marine photobioreactors, which have been the subject of extensive and innovative work (for example, see Contreras et al, 1998;Hu et al, 1998;Matsunaga and Burgess, 1994). Many marine bac- (Karamanev and Nikolov, 1991) High biomass concentrations Little loss of cells through washout (Godia et al, 1987) Improved mass transfer Reduced media viscosity, increased differential velocity between media and cells (Wang et al, 1984) Advantageous partitioning effects Support can decrease inhibitory product concentration near cells (Wada et al, 1981) Increased product yield Energy is channelled away from biomass synthesis, into product synthesis (Sayles and Ollis, 1989) Increased product stability Liquid throughput reduces a product's residence time and therefore possibility of degradation (Okada et al, 1987) Integration with downstream processing Cells easily separated from media and products (Furusaki, 1988) Increased reaction selectivity Immobilization can affect membrane permeability (Watanabae et al, 1988) Flexibility in terms of fermentation protocol…”

Section: Bioreactor Considerationsmentioning

confidence: 99%

Bioprocess Intensification for Production of Novel Marine Bacterial Antibiotics Through Bioreactor Operation and Design

1999

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: There is a lack of research into bioreactor engineering and fermentation protocol design in the field of marine bacterial antibiotic production. Most production strategies are carried out at the shake-flask level and lack a mechanistic understanding of the antibiotic production process, offering poor prospects for successful scale-up. This review shows that data need to be collated on media and physical optima differences between the trophophase and idiophase, along with investigations into the control mechanisms for biosynthesis, to allow implementation of novel fermentation protocols. Immobilization may play a part in bioprocess intensification of marine bacterial antibiotic production, through again this area is understudied. Similarly, mass transfer and shear stress data of fermentations are needed to provide the bioreactor design requirements to intensify antibiotic biosynthesis, with process scale-up in mind. The application of bioprocess intensification methods to the production of antibiotics (and other metabolites) from marine microbes will become an important strategy for improving supply of natural products, in order to assess their suitability as chemotherapeutic drugs.

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Section: Bioreactor Considerationsmentioning

confidence: 99%

Bioprocess Intensification for Production of Novel Marine Bacterial Antibiotics Through Bioreactor Operation and Design

1999

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“…A escolha do procedimento deve considerar a relação custos vs eficiência. 3,4,50,56,57,74 Se o hidrolisado protéico assim obtido ainda apresentar características organoléticas indesejáveis, recomenda-se a realização da reação de plasteína, que consiste na condensação peptídeo-peptídeo, útil para a remoção do paladar amargo e para a incorporação de tirosina e triptofano, parcialmente perdidos durante a hidrólise enzimática. [25][26][27]56,77,78 Esses dois aminoácidos, liberados juntamente com a Phe, são importantes, pois são aminoácidos essenciais.…”

Section: Hidrolisados Protéicos Com Baixo Teor De Pheunclassified

Importância do diagnóstico e tratamento da fenilcetonúria

Mira

Marquez

2000

Rev. Saúde Pública

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A fenilcetonúria (PKU) é o mais comum dos erros congênitos do metabolismo de aminoácidos. Resulta da deficiência da fenilalanina hidroxilase, enzima que catalisa a conversão de fenilalanina em tirosina. A introdução de uma dieta com baixo teor de fenilalanina deve ter início nos primeiros meses de vida, de preferência no primeiro mês, para evitar o retardo mental, manifestação clínica mais severa da doença. Foi elaborada revisão sobre essa temática, que aborda desde a PKU clássica até a hiperfenilalaninemia branda, incluindo relato sobre a PKU maternal e os efeitos da exposição do útero a altos níveis de fenilalanina sobre o feto.

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“…The different permeabilities of various substances in a complex reaction medium may be exploited in such a way that the selectivity of a particular reaction is increased. Intact cells of Rhodotorula rubra indiscriminately consumed all the constituents of an enzymic hydrolysate of a whey protein preparation, but cells entrapped in a polypyrrole membrane matrix consumed only low-molecular-weight components to give a low-phenylalanine peptide mixture in a high yield (Watanabe et al, 1988).…”

Section: The Increase In Reaction Selectivitymentioning

confidence: 99%

Theoretical Studies on Immobilised Cell Fermentation Technology

Δερβακοσ¹

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A critical examination is made of the many experimental and theoretical studies relating to the use of immobilised metabolically-intact whole cells to carry out biological reactions -a technique which can be defined as Immobilised Cell Fermentation Technology (ICFT).The first part of the work was mainly designed to consolidate the published work in the form of a computer package, including database and mathematical modules. Application of the package has shown that while most of the advantages which are often claimed for the technology have been verified experimentally, there is still much scope for revising the form of the standard diffusion-reaction equations which are ordinarily applied to describe the behaviour of these systems. It was illustrated that application of such models may lead to disparities between theoretical and empirical data. The observed differences were also discussed in the light of experimental limitations.This study illustrates the need to take into account the particular characteristics of immobilised viable cells when modelling their behaviour. George A. Dervakos Ph.D Thesis UMIST 1990 Declaration None of the material contained in this Thesis has been submitted in support of an application for another degree or qualification of this or any other university or other institute of learning. George A. Dervakos 3.2.6 The increased product stability 57 3.2.7 The integration with downstream processing 58 3.2.8 Advantages due to cell proximity 59 3.2.9 The increase in reaction selectivity 3.2.10 The versatility in the selection of the reactor 60 The metabolic changes in immobilised cells 61 Problems introduced by cell immobilisation 68 3.4.1 Mechanical problems 69 3.4.2 Increased substrate limitations 73 3.4.3 Increased product inhibition 74 Conclusions APPLICATION OF THE DESIGN MODULE: A PRELIMINARY LOOK AT INTRA-PARTICLE DIFFUSION LIMITATIONS IN IMMOBILISED CELL SYSTEMS Introduction Ethanol production with immobilised cells 79 Oxygen transport in immobilised cell aggregates Cellulase production using cells immobilised in biomass support particles . . 94 Conclusions FURTHER EXAMINATION OF EFFECTIVE DD7FUSIVITIES .... Introduction The structure of the immobilised cell aggregate 5.2.1 The volume fraction of cells within the aggregate 5.2.2 The volume fraction of cell clusters within the aggregate 5.2.3 The volume fraction of carrier within the aggregate 5.2.4 Some idiosyncrasies of the structure of the immobilised cell aggregate Diffusive flux in the immobilised cell aggregate 5.3.1 The definition of the effective diffusivity 5.3.2 Experimental work xii 5.4 The errors and misunderstandings 118 5.5 Summary 128 CHAPTER 6: FURTHER EXAMINATION OF DIFFUSION/REACTION THEORY . 6.1Table 6.4: Parametric values used in the dynamic simulation in Fig 6.37 (linear inhibition)

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Construction of a Bioreactor with Immobilized Yeast Cells for Production of a Low-phenylalanine Peptide Mixture as a Phenylketonuria Foodstuff

Cited by 3 publications

References 5 publications

Bioprocess Intensification for Production of Novel Marine Bacterial Antibiotics Through Bioreactor Operation and Design

Bioprocess Intensification for Production of Novel Marine Bacterial Antibiotics Through Bioreactor Operation and Design

Importância do diagnóstico e tratamento da fenilcetonúria

Theoretical Studies on Immobilised Cell Fermentation Technology

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