Construction of a cDNA library and preliminary analysis of expressed sequence tags in Piper hainanense

Abstract

ABSTRACT. Black pepper is a perennial climbing vine. It is widely cultivated because its berries can be utilized not only as a spice in food but also for medicinal use. This study aimed to construct a standardized, highquality cDNA library to facilitated identification of new Piper hainanense transcripts. For this, 262 unigenes were used to generate raw reads. The average length of these 262 unigenes was 774.8 bp. Of these, 94 genes (35.9%) were newly identified, according to the NCBI protein database. Thus, identification of new genes may broaden the molecular knowledge of P. hainanense on the basis of Clusters of Orthologous Groups and Gene Ontology categories. In addition, certain basic genes linked to physiological processes, which can contribute to disease resistance and thereby to the breeding of black pepper. A total of 26 unigenes were found to be SSR markers. Dinucleotide SSR was the main repeat motif, accounting (2015) for 61.54%, followed by trinucleotide SSR (23.07%). Eight primer pairs successfully amplified DNA fragments and detected significant amounts of polymorphism among twenty-one piper germplasm. These results present a novel sequence information of P. hainanense, which can serve as the foundation for further genetic research on this species.

Piper Species: A Comprehensive Review on Their Phytochemistry, Biological Activities and Applications

“…The part of Piper hainanense above the ground has been utilized in Chinese folk medicine to alleviate pain, relieve symptoms of asthma and treat bacterial infections [226].…”

Multiple-Genome-Based Simple Sequence Repeat Is an Efficient and Successful Method in Genotyping and Classifying Different Jujube Germplasm Resources

“…Identifying core repeat units and designing specific primers are essential for the detection of microsatellite (SSR) molecular markers [1,26]. Traditional SSR screening approaches, such as gene library, interspecies transfer amplification, and public database searches, were time-consuming and inefficient [27][28][29]. Additionally, gel electrophoresis, which is the conventional method to identify SSR typing results, has limitations such as poor resolution and limited capacity for high-throughput screening [30].…”