Construction of a comparative genetic map in faba bean (Vicia faba L.); conservation of genome structure with Lens culinaris

Abstract

Background: The development of genetic markers is complex and costly in species with little preexisting genomic information. Faba bean possesses one of the largest and least studied genomes among cultivated crop plants and no gene-based genetic maps exist. Gene-based orthologous markers allow chromosomal regions and levels of synteny to be characterised between species, reveal phylogenetic relationships and chromosomal evolution, and enable targeted identification of markers for crop breeding. In this study orthologous codominant cross-species markers have been deployed to produce the first exclusively gene-based genetic linkage map of faba bean (Vicia faba), using an F 6 population developed from a cross between the lines Vf6 (equina type) and Vf27 (paucijuga type).

Use of synteny to identify candidate genes underlying QTL controlling stomatal traits in faba bean (Vicia faba L.)

“…Marker-assisted selection (MAS) in faba bean is less advanced than in some other legume species, but significant efforts have been made to enrich genetic and genomic resources in this crop (Torres et al 2010Alghamdi et al 2012), fortified by comparative genomics with M. truncatula and other legumes (Ellwood et al 2008;Kaur et al 2012;Torres et al 2012;Khazaei et al 2014). Genomic and transcriptomic approaches, now being applied in faba bean, open new opportunities for fine mapping or uncovering of candidate genes.…”

Legume Crops Phylogeny and Genetic Diversity for Science and Breeding

“…A high throughput SNP genotyping array targeting 887 loci has been developed for genomic-assisted breeding in faba bean (Cottage et al, 2012). Limited gene sequence homologies and synteny to Medicago truncatulla have been applied to anchor gene-based markers in the faba bean and lentil linkage groups (Ellwood et al, 2008). However, genomewide comparison between these two species has not yet been carried out due to the scarcity of faba bean genome sequence data.…”

Genetic mapping and annotation of genomic microsatellites isolated from globe artichoke

“…As previously performed in the Solanaceae (Wu et al 2006(Wu et al , 2009, Fabaceae (Phan et al 2007;Hougaard et al 2008;Ellwood et al 2008) and Asteraceae (Chapman et al 2007) families, a COS marker approach may represent an effective mean for generating molecular markers. Our comparative analysis amongst the Asteraceae species showed similarity values up to 100% between sequences from globe artichoke and those from the yellow starthistle (Centaurea solstitialis) or safflower (Carthamus tinctorius); accordingly, an exploration of the Asteraceae dbEST seems very promising for new microsatellite markers mining as well as for synteny studies.…”