Construction of a core collection of native Perilla germplasm collected from South Korea based on SSR markers and morphological characteristics

Abstract

The leaves and seed oil of Perilla crop (Perilla frutescens L.) have attracted interest as health foods in East Asia. This crop has been traditionally cultivated and used for a long time as a folk plant, especially in Korea. In our study, the 22 SSR markers and eight morphological traits were used to assess the genetic diversity and population structure, to select a core collection of 400 Perilla accessions conserved in the RDA-Genebank of South Korea. A total of 173 alleles were detected and the number of alleles per locus ranged from 4 to 15 (average = 7.9). Gene diversity and polymorphic information content ranged from 0.138 to 0.868 (average = 0.567) and 0.134 to 0.853 (average = 0.522), respectively. The 400 accessions were not clearly distinguished geographically by STRUCTURE and UPGMA analyses. A core collection (44 accessions) was selected from the entire collection by using PowerCore. The core collection accounted for 11.0% of the entire Perilla collection, including 100% of the number of alleles maintained in the whole collection and with similar or greater Shannon–Weaver and Nei diversity indices than the whole collection. The core collection selected by SSR markers was evenly distributed in three clusters on a scatter plot by eight morphological traits. The first core collection of Perilla accessions was constructed, and it maintained allelic richness. Further modification of the core collection is expected with the continuous addition of new accessions of the two cultivated types of Perilla crop and their weedy types.

Utilization of Novel Perilla SSR Markers to Assess the Genetic Diversity of Native Perilla Germplasm Accessions Collected from South Korea

“…frutescens , which is used as an oil, condiment, and vegetable (for wraps and salads) crop; and cultivated var. crispa , which is used as a Chinese herbal medicine and vegetable (for pickles and salads) crop [ 1 , 2 , 3 , 4 , 5 ]. Today, these two varieties (var.…”

“…frutescens is extensively cultivated and used mainly in South Korea, while var. crispa is extensively cultivated and used mainly in Japan [ 1 , 2 , 3 , 5 , 6 , 7 ]. However, the origin of the Perilla crop is believed to be China, because China has a long cultivation history, high genetic diversity, and many resources distributed in the country [ 1 , 2 , 3 , 4 , 8 , 9 , 10 ].…”

“…In the Perilla crop, molecular marker technology has recently provided more comprehensive information on the genetic variation and phylogenetic relationships between and within accessions of the CWTPC. Molecular markers such as randomly amplified polymorphic DNA (RAPD) [ 3 , 12 ], amplified fragment length polymorphism (AFLP) [ 2 , 6 ], and simple sequence repeat (SSR) or microsatellite [ 5 , 7 , 19 , 20 , 21 , 22 ] have been successfully applied to accessions of the CWTPC, providing rich genetic diversity and genetic relationship information for genetic research of the Perilla crop. Among them, SSR markers are considered to be the preferred choices of markers for genetic studies because of their codominance, polymorphism, high repeatability, and rich genetic information [ 20 , 23 ].…”

First assessment of Iranian pomegranate germplasm using targeted metabolites and morphological traits to develop the core collection and modeling of the current and future spatial distribution under climate change conditions

“…This reveals that the diversity in the core collection has increased. In the core collection established from the germplasm of the Perilla frutescens L. was also observed that the averages of Shannon and Nei diversity indices in the Core collection were higher than those in the total collection [99]. In can be concluded that the constructed core collection facilitates experiments to evaluate germplasm under different environmental conditions and the breeding programs in the future.…”

Genome-Wide Association Study for Agro-Morphological Traits in Eggplant Core Collection

“…The genetic diversity of plant genetic resources (PGRs), which provide useful alleles linked to plant development and improvement, is critical for the conservation and utilization of germplasm conserved in a gene bank [ 21 , 22 ]. DNA molecular markers provide valuable information for analyzing genetic diversity, genetic relationships, population structure, and core collections in a variety of crop species [ 23 , 24 , 25 , 26 , 27 , 28 ]. Representative core collections have been selected in various crops using different sampling strategies and clustering methods [ 29 , 30 , 31 , 32 , 33 , 34 ].…”