Construction of a focal adhesion signaling pathway-related ceRNA network in pelvic organ prolapse by transcriptome analysis

Xia, Yu; He, Li; Chen, Ying; Wang, Lin; Liu, Hong; Yang, Xiaomin; Ye, Ying; Zheng, Xuemei; Yang, Zhenglin; Lin, Yonghong

doi:10.3389/fgene.2022.996310

Cited by 5 publications

(5 citation statements)

References 42 publications

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“…Focal adhesion is the close-fitting linking between the cell and the extracellular matrix that allows their communication and signaling [98]. It includes a complex of junctional sign proteins that introduce the actin cytoskeleton to the integrins, and it controls several cellular processes like relocation, proliferation, distinction, apoptosis, and gene expression [99]. According to the focal adhesion sensitivity to mechanical forces, it regulates its construction and role [100].…”

Section: Discussionmentioning

confidence: 99%

Analysis and Review of Downregulated Actin Cytoskeletal Proteins in Non-Small Cell Lung Cancer

Mageed,

Sahu,

Sundararajan

2024

JBM

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Actin, a highly conserved protein, plays a dominant role in Non-small cell lung cancer (NSCLC). Late diagnosis and the aggressive nature of NSCLC pose a significant threat. Studying the clinic pathological properties of NSCLC proteins is a potential alternative for developing treatment strategies. Towards this, 35 downregulated actin cytoskeletal proteins on NSCLC prognosis and treatment were studied by examining their protein-protein interactions, gene ontology enrichment terms, and signaling pathways. Using PubMed, various proteins in NSCLC were identified. The protein-protein interactions and functional associations of these proteins were examined using the STRING database. The focal adhesion signaling pathway was selected from all available KEGG and Wiki pathways because of its role in regulating gene expression, facilitating cell movement and reproduction, and significantly impacting NSCLC. The protein-protein interaction network of the 35 downregulated actin cytoskeleton proteins revealed that ACTG1, ACTR2,

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Section: Discussionmentioning

confidence: 99%

Analysis and Review of Downregulated Actin Cytoskeletal Proteins in Non-Small Cell Lung Cancer

Mageed,

Sahu,

Sundararajan

2024

JBM

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“…Moreover, ECM2 can be used as a prognostic biomarker for low-grade gliomas, and high ECM2 expression promotes glioma proliferation, migration, and invasion ( 55 , 56 ). PPP1R12B is involved in the regulation of cell-matrix adhesion dynamics and traction force turnover ( 57 , 58 ). EMILIN1, an adhesive ECM glycoprotein, forms stable intra-chain salt bridges with α4β1 integrins, which enhance cell adhesion ( 59 ).…”

Section: Discussionmentioning

confidence: 99%

RNA 5-Methylcytosine regulators are associated with cell adhesion and predict prognosis of endometrial cancer

Yang,

Luo,

Zhou

et al. 2023

Transl Cancer Res

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Background RNA methylation is a significant form of post-transcriptional modification that has been implicated in various diseases, including cancers. One prominent type of RNA methylation is 5-Methylcytosine (m 5 C), which primarily regulates RNA stability, transcription, and translation. However, the role of m 5 C-related gene regulation in cell adhesion within uterine corpus endometrial carcinoma (UCEC) remains unexplored. Therefore, the objective of this study was to investigate the association between RNA m 5 C methylation and UCEC and develop a prognostic predictive model to forecast survival outcomes in UCEC patients. Methods The RNA datasets were acquired from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. The dataset was used to explore the interaction relationships of m 5 C regulators in UCEC. Unsupervised clustering analysis identified clusters with distinct m 5 C modification patterns. Different clusters underwent Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment level analysis to investigate the effects of pathways related to m 5 C methylation, which were further validated through in vitro cellular experiments. A prognostic predictive model was developed using the least absolute shrinkage and selection operator (LASSO) and multivariate regression analysis. Results Two clusters with distinct m 5 C modification patterns were identified using unsupervised cluster analysis. Furthermore, the prognosis of cluster 2 was found to be worse. Enrichment analysis showed alterations in cell adhesion-related pathways in both clusters, as well as differences between the clusters. Through this analysis, we identified 25 genes with significant prognostic value. Finally, a prognostic predictive model comprising NSUN2 and YBX1 was constructed. Conclusions In conclusion, diverse m 5 C modification patterns display distinct cell adhesion properties in UCEC, which are correlated with prognosis and offer significant potential as prognostic markers for UCEC assessment. We developed a prognostic predictive model to accurately predict the prognosis of UCEC.

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“…The adhesion of POP-HVFs to the ECM was decreased, resulting in impaired tissue morphology at the microstructural level. In terms of cellular morphology, a separate study revealed severe disruption of fibroblast cytoskeletal actin filaments in fibroblasts from POP patients, suggesting a close association between abnormalities in this cellular support protein and external mechanical stretching ( 24 ). Besides, increasing evidence suggests that the vaginal microbiome and the immune system are closely linked to maintain a healthy vaginal environment and lower genitourinary health ( 25 ).…”

Section: Pop and Biomechanicsmentioning

confidence: 99%

Roles and mechanisms of biomechanical-biochemical coupling in pelvic organ prolapse

Wu,

Zhang,

et al. 2024

Front. Med.

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Pelvic organ prolapse (POP) is a significant contributor to hysterectomy among middle-aged and elderly women. However, there are challenges in terms of dedicated pharmaceutical solutions and targeted interventions for POP. The primary characteristics of POP include compromised mechanical properties of uterine ligaments and dysfunction within the vaginal support structure, often resulting from delivery-related injuries. Fibroblasts secrete extracellular matrix, which, along with the cytoskeleton, forms the structural foundation that ensures proper biomechanical function of the fascial system. This system is crucial for maintaining the anatomical position of each pelvic floor organ. By systematically exploring the roles and mechanisms of biomechanical-biochemical transformations in POP, we can understand the impact of forces on the injury and repair of these organs. A comprehensive analysis of the literature revealed that the extracellular matrix produced by fibroblasts, as well as their cytoskeleton, undergoes alterations in patient tissues and cellular models of POP. Additionally, various signaling pathways, including TGF-β1/Smad, Gpx1, PI3K/AKT, p38/MAPK, and Nr4a1, are implicated in the biomechanical-biochemical interplay of fibroblasts. This systematic review of the biomechanical-biochemical interplay in fibroblasts in POP not only enhances our understanding of its underlying causes but also establishes a theoretical foundation for future clinical interventions.

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Construction of a focal adhesion signaling pathway-related ceRNA network in pelvic organ prolapse by transcriptome analysis

Cited by 5 publications

References 42 publications

Analysis and Review of Downregulated Actin Cytoskeletal Proteins in Non-Small Cell Lung Cancer

Analysis and Review of Downregulated Actin Cytoskeletal Proteins in Non-Small Cell Lung Cancer

RNA 5-Methylcytosine regulators are associated with cell adhesion and predict prognosis of endometrial cancer

Roles and mechanisms of biomechanical-biochemical coupling in pelvic organ prolapse

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