Construction of a Linkage Map in Cucumis Melo (L.) Using Random Amplified Polymorphic Dna Markers

Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.)

“…That map consisted of 14 major and six minor linkages groups composed of 197 AFLPs, six RAPDs, and one SSR marker, spanning 1942 cM, with an average distance between markers of 11 cM Dean 1997, 1998). Similarly, Liou et al (1998) used 64 F 2 progeny (Makuna #SLK-V-052X"Sky Rocket") and 125 RAPD markers to construct a map consisting of twenty-nine linkage groups spanning 1348 cM, with markers spaced at an average distance of 10.8 cM. Staub, Meglic, and McCreight (1998) also used F 2 and BC 1 progenies originating from strategic crosses among 400 USDA Pis to construct two linkage groups consisting of 11 isozyme loci spaced at an average distance of 9 cM.…”

“…Most melon maps have been developed from F 2 or BC 1 populations, which are not particularly wellsuited for extensive replicated, multi-location evaluation (Baudracco-Arnas and Pitrat 1996; Liou et al 1998;Wang, Thomas, and Dean 1998;Oliver et al 2001;Danin-Poleg et al 2002;Silberstein et al 2003). Although immortalized populations (e.g., DH and RIL populations) are ideal for analyzing complex trait QTL, saturated melon maps constructed from such populations are relatively new (Perin et al 2002a,b,c;Gonzalo et al 2005).…”

“…However, many initial melon maps included many dominant markers (Baudracco-Arnas and Pitrat 1996; Liou et al 1998;Wang, Thomas, and Dean 1998;Danin-Poleg et al 2002;Silberstein et al 2003) with information that is usually not readily transferable to other populations (Perin et al 2002a). Codominant markers (e.g., SSRs) are often syntenic and can be used in map comparison and merging experiments (Danin-Poleg et al 2001;Gonzalo et al 2005).…”

A reference map of Cucumis melo based on two recombinant inbred line populations

“…More than 109 genes have been described in the current gene list for C. melo (Pitrat 1998), and classical genetic linkage maps with a few morphological and disease-resistance genes (Pitrat 1991), or isozyme markers (Staub et al 1998) exist. In addition, several partial maps with molecular markers have been published: on 244 F 2 individuals with 110 RFLP (restriction fragment length polymorphism) and RAPD (random amplified polymorphic DNA) markers (Baudracco-Arnas and Pitrat 1996), on 66 BC 1 individuals with 196 AFLP (amplified fragment length polymorphism) markers (Wang et al 1997), on 77 F 2 plants with 125 RAPD markers (Liou et al 1998), on 64 F 2 individuals with 107 markers (RAPDs, RFLPs, Inter Microsatellite Amplification = IMA and Simple Sequence Repeats = SSRs) (Brotman et al 2000), on 93 F 2 individuals with 411 markers (RFLPs, AFLPs, RAPDs, SSRs and isozymes) (Oliver et al 2001).…”

Genetic Mapping and Molecular Breeding in Cucurbits

“…For example, in melon, MR-1 used by Wang et al (1997) was resistant to fusarium wilt (Fom-1 and Fom-2), downy and powdery mildews; Vedrantais and PI 161375 used by Baudarcco-Arnas and Pitrat (1996) and Périn et al (1998Périn et al ( , 2002a were resistant to fusarium wilt (Fom-1 and Fom-2), and melon necortic spot virus (nsv), and aphid (Vat), respectively; PI 161375 × Pinyonet Piel de Sapo used by Oliver et al (2001) segregated for both nsv and Vat . Populations used by Liou et al (1998), Fukino et al (2002, and Silberstein et al (2003) also segregated for disease resistance in addition to other horticultural traits. These intensive efforts have paid off, as we will see in the following sections that quite a number of disease resistance genes are being mapped by tightly linked markers that will be extremely useful in molecular breeding.…”