Construction of a non-enzymatic electrochemical sensor based on graphitic carbon nitride nanosheets for sensitive detection of procalcitonin

Liu, Yushuang; Chen, Fu‐Rong; Bao, Layue; Hai, Wenfeng

doi:10.1039/d2ra03650a

Cited by 10 publications

(7 citation statements)

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“…For the g-C 3 N 4 , they appeared layered, rippled and resembled waves of crumpled silk veil, illustrating the nanosheets structure (Figure 1A,D). Our previous research identified that this special nanosheet structure could provide more active binding sites [35]. For the NiCoP/g-C 3 N 4 nanocomposites, there were several small nanoparticles displayed on the g-C 3 N 4 surface, implying that NiCoP was successfully loaded onto the g-C 3 N 4 surface (Figure 1B,E).…”

Section: The Morphology and Structural Informationmentioning

confidence: 87%

“…XRD was used to distinguish the phases and the structures of bare g-C 3 N 4 and NiCoP loading. As shown in Figure 2, the lattice planes of triazine units (100) (13.1 • ) and the lattice planes of interlayer stacking of aromatic segments (002) (27.8 • ) were detected, indicating the bare g-C 3 N 4 was successfully synthesized [35,39]. For the NiCoP/g-C 3 N 4 samples, beside the strongest diffraction peaks of g-C 3 N 4 , several weak peaks appeared at 41 • , 44.95 • and 47.97 • , which correspond to the (111), ( 201) and (210) lattice planes of NiCoP, respectively [40,41] (JCPDS No.…”

Section: The Morphology and Structural Informationmentioning

confidence: 93%

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NiCoP/g-C3N4 Nanocomposites-Based Electrochemical Immunosensor for Sensitive Detection of Procalcitonin

Chen¹,

Bao²,

Zhang³

et al. 2023

Sensors

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Herein, an ultra-sensitive and facile electrochemical biosensor for procalcitonin (PCT) detection was developed based on NiCoP/g-C3N4 nanocomposites. Firstly, NiCoP/g-C3N4 nanocomposites were synthesized using hydrothermal methods and then functionalized on the electrode surface by π-π stacking. Afterward, the monoclonal antibody that can specifically capture the PCT was successfully linked onto the surface of the nanocomposites with a 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS) condensation reaction. Finally, the modified sensor was employed for the electrochemical analysis of PCT using differential Pulse Voltammetry(DPV). Notably, the larger surface area of g-C3N4 and the higher electron transfer capacity of NiCoP/g-C3N4 endow this sensor with a wider detection range (1 ag/mL to 10 ng/mL) and an ultra-low limit of detection (0.6 ag/mL, S/N = 3). In addition, this strategy was also successfully applied to the detection of PCT in the diluted human serum sample, demonstrating that the developed immunosensors have the potential for application in clinical testing.

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Section: The Morphology and Structural Informationmentioning

confidence: 87%

Section: The Morphology and Structural Informationmentioning

confidence: 93%

NiCoP/g-C3N4 Nanocomposites-Based Electrochemical Immunosensor for Sensitive Detection of Procalcitonin

Chen¹,

Bao²,

Zhang³

et al. 2023

Sensors

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“…The probe peptide (PP) is connected to the electrode surface through π-π stacking between g-C 3 N 4 NS and phenylalanine at the end of the peptide, providing efficient electrochemical solutions for ultrasensitive detection of PCT (Fig. 5 C) [ 56 ]. An ultrasensitive sandwich electrochemical method was employed for PCT testing.…”

Section: Chemiluminescent Immunoassay For Pct Detectionmentioning

confidence: 99%

Section: Chemiluminescent Immunoassay For Pct Detectionmentioning

confidence: 99%

Progress in Procalcitonin Detection Based on Immunoassay

Huang,

Zu,

Zhang

et al. 2024

Research

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Procalcitonin (PCT) serves as a crucial biomarker utilized in diverse clinical contexts, including sepsis diagnosis and emergency departments. Its applications extend to identifying pathogens, assessing infection severity, guiding drug administration, and implementing theranostic strategies. However, current clinical deployed methods cannot meet the needs for accurate or real-time quantitative monitoring of PCT. This review aims to introduce these emerging PCT immunoassay technologies, focusing on analyzing their advantages in improving detection performances, such as easy operation and high precision. The fundamental principles and characteristics of state-of-the-art methods are first introduced, including chemiluminescence, immunofluorescence, latex-enhanced turbidity, enzyme-linked immunosorbent, colloidal gold immunochromatography, and radioimmunoassay. Then, improved methods using new materials and new technologies are briefly described, for instance, the combination with responsive nanomaterials, Raman spectroscopy, and digital microfluidics. Finally, the detection performance parameters of these methods and the clinical importance of PCT detection are also discussed.

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“…It is composed of 116 amino acids and is produced by parafollicular cells (C cells) of the thyroid and by the neuroendocrine cells of the lung and the intestine. 10,11 Early differentiation between septic and non-septic arthritis and the possible decision for therapy remains a difficult task for the physician because clinical signs and traditional markers of infection are of limited value. 12,13 Arthrocentesis with synovial gram stain and culture is considered as gold standard, however, the sensitivity of the gram stain is only 50-75% and culture is not available immediately.…”

Section: Introductionmentioning

confidence: 99%

The Role of Vitamin D-Binding Protein, and Procalcitonin in Patients with Arthritis on Vitamin D

Obied,

Sarhat

2024

Pharmacogn J.

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Background: Arthritis is a term often used to mean any disorder that affects joints. Symptoms generally include joint pain and stiffness. Other symptoms may include redness, warmth, swelling, and decreased range of motion of the affected joints. In some types of arthritis, other organs are also affected. Aim: This study aimed to identify the impact of vitamin D therapy on serum level of vitamin D-binding protein and procalcitonin in Patients with arthritis. Methods: This cross-sectional study, was conducted in Kirkuk city between January 1 st and March 1 st , 2024. A total of 180 subjects were included, categorized into three groups: arthritis patients not receiving vitamin D (Group 1), arthritis patients receiving vitamin D for at least 2 months (Group 2), and a control group comprising 40 healthy subjects. Blood samples were collected from participants, processed, and stored for subsequent analysis. Various biomarkers, including vitamin D-binding protein and procalcitonin, were determined using ELISA kits. Results: The study revealed a higher prevalence of females among arthritis patients (54.29%), with most affected individuals aged above 60 years. Urban residency was predominant among arthritis patients (75.71%). The majority of RA patients had been affected for 6-10 years (40%). Comparative analysis demonstrated significantly higher procalcitonin levels in RA patients without vitamin D supplementation (112.4±24.3 ng/ ml) compared to those with supplementation (48.33±10.73 ng/ml) and healthy controls (9.68±5.49 ng/ ml). Furthermore, vitamin D binding protein levels were significantly lower in arthritis patients without supplementation (1.26±0.12 ng/ml) compared to those with supplementation (0.75±0.15 ng/ml) and healthy controls (0.23±0.14 ng/ml). Conclusion: These findings underscore the potential role of vitamin D supplementation in modulating inflammatory markers and enhancing vitamin D binding protein levels in arthritis patients, suggesting its therapeutic implications in disease management.

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Construction of a non-enzymatic electrochemical sensor based on graphitic carbon nitride nanosheets for sensitive detection of procalcitonin

Abstract: A simple and ultra-sensitive electrochemical biosensor based on graphitic carbon nitride nanosheets (g-C3N4 NS) was developed for the detection of PCT. This sensor presented excellent sensing performance and demonstrates potential for clinical application.

Cited by 10 publications

References 50 publications

NiCoP/g-C3N4 Nanocomposites-Based Electrochemical Immunosensor for Sensitive Detection of Procalcitonin

NiCoP/g-C3N4 Nanocomposites-Based Electrochemical Immunosensor for Sensitive Detection of Procalcitonin

Progress in Procalcitonin Detection Based on Immunoassay

The Role of Vitamin D-Binding Protein, and Procalcitonin in Patients with Arthritis on Vitamin D

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