Construction of a novel synthetic root-specific promoter and its characterization in transgenic tobacco plants

Mohan, Chakravarthi; Jayanarayanan, Ashwin Narayan; Narayanan, Subramonian

doi:10.1007/s13205-017-0872-9

Cited by 23 publications

(18 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, large-scale prediction of numerous cis-acting elements involved in plant hormones 97 , calcium 98 , and biotic and abiotic stress responses were performed in Arabidopsis through global analysis of gene expression 99,100 . However, limited information available regarding root specific promoters 101,102 and root-associated regulatory elements 103 with the exception of a few well-known root specific motifs like ROOTMOTIFTAPOX1 element found in promoters of rolD 104 . In this study, the genome-wide analyses using available Brachypodium genome sequence and our time-dependent transcriptomic data revealed several putative cis-elements within promoters of the genes encoding TFs associated with root growth during plant developmental stages.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of Global Gene Expression and Regulatory Elements in Growing Brachypodium Root System

Ogden

Wietsma

Winkler

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Root systems are dynamic and adaptable organs that play critical roles in plant development. However, how roots grow and accumulate biomass during plant life cycle and in relation to shoot growth phenology remains understudied. A comprehensive time-dependent root morphological analysis integrated with molecular signatures is then required to advance our understanding of root growth and development. Here we studied Brachypodium distachyon rooting process by monitoring root morphology, biomass production, and c/n ratios during developmental stages. to provide insight into gene regulation that accompanies root growth, we generated comprehensive transcript profiles of Brachypodium whole-root system at four developmental stages. our data analysis revealed that multiple biological processes including trehalose metabolism and various families of transcription factors (TFs) were differentially expressed in root system during plant development. In particular, the AUX/iAA, eRfs, WRKY, nAc, and MADS tf family members were upregulated as plant entered the booting/heading stage, while ARfs and GRfs were downregulated suggesting these tf families as important factors involved in specific phases of rooting, and possibly in regulation of transition to plant reproductive stages. We identified several Brachypodium candidate root biomass-promoting genes and cis-regulatory elements for further functional validations and root growth improvements in grasses. Roots are the central core of the plant system which play a critical role during plant growth and development. Although root size and shape can be modified by environmental factors, the genotype and internal phenotype of an individual (at cellular and molecular levels) sets the limits within which such modification of growth and development can occur 1. Our knowledge of the physiological basis underlying root growth and development and the relevant variations in belowground biomass production is poor, but should start with providing a basic understanding of the phenotypic and molecular determinants of global root architecture over the course of plant lifetime. Grasses are critical components of providing global food and bioenergy. Improving the productivity of monocotyledonous flowering crops like wheat, rice, sorghum, etc. (Poaceae family) is therefore vital to keep pace with population growth. This requires a fundamental understanding of molecular physiology of organ development (e.g. leaf, flower, root, etc.) during the crop life cycle. Recently, Brachypodium distachyon (Poaceae, henceforth Brachypodium) has been chosen as a model system to study different aspects of temperate grasses because of its short lifecycle, relatively simple diploid genome 2 , and manageable growth requirements 3. The vegetative and reproductive growth stages of B. distachyon have been thoroughly studied based on phenological characteristics of above-ground tissues 4. Our limited knowledge in the area of grass root biology is partly due to the complex fibrous root system characteristic of monocots comp...

show abstract

Section: Discussionmentioning

confidence: 99%

Dynamics of Global Gene Expression and Regulatory Elements in Growing Brachypodium Root System

Ogden

Wietsma

Winkler

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Thus, the multiplication of the elements, their reorganization, and ligation with native and synthetic cis -sequences are used to obtain synthetic promoters. The development and use of such a novel promoter can successfully regulate transgenes in trait- and environment-specific manners (Venter and Botha 2010; Dey et al, 2015; Biłas et al, 2016; Mohan et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

A Fruitful Decade Using Synthetic Promoters in the Improvement of Transgenic Plants

2019

View full text Add to dashboard Cite

Advances in plant biotechnology provide various means to improve crop productivity and greatly contributing to sustainable agriculture. A significant advance in plant biotechnology has been the availability of novel synthetic promoters for precise spatial and temporal control of transgene expression. In this article, we review the development of various synthetic promotors and the rise of their use over the last several decades for regulating the transcription of various transgenes. Similarly, we provided a brief description of the structure and scope of synthetic promoters and the engineering of their cis-regulatory elements for different targets. Moreover, the functional characteristics of different synthetic promoters, their modes of regulating the expression of candidate genes in response to different conditions, and the resulting plant trait improvements reported in the past decade are discussed.

show abstract

“…Synthetic promoters have mainly been constructed using cis-regulatory elements that bind to core promoters [ 12 ]. The construction of different pathogen-inducible promoters may effectively improve the disease resistance in transgenic plants [ 11 , 13 ]. Alternatively, constructing an inducible promoter in combination with a tissue-specific promoter (e.g., root, stem, leaf) and inducible promoter contributes to specific tissue-induced expression to improve crop quality, crop robustness, and disease resistance [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Construction and characterization of a synthetic Baculovirus-inducible 39K promoter

Dong

et al. 2018

J Biol Eng

View full text Add to dashboard Cite

BackgroundSilkworm genetic engineering is widely used in gene function, silk engineering and disease-resistant engineering in most of Asia. Some of the earliest promoter elements are used to control the development of silkworm transgenic expression and gene therapy. However, the low expression and specificity of natural promoters limit the applications of genetic engineering. To construct a highly efficient synthetic inducible promoter in the Bombyx mori (Lepidoptera), we analyzed the regulatory elements and functional regions of the B. mori nucleopolyhedrovirus 39 K promoter.ResultsTruncated mutation analysis of the 39 K promoter showed that the transcriptional regulatory region spanning positions − 573 to − 274 and + 1 to + 62 are essential for virus-inducible promoter activity. Further investigations using the electrophoretic mobility shift assay revealed that the baculovirus IE-1 protein binds to the 39 K promoter at the − 310 to − 355 region, and transcription activates the expression of 39 K promoter assay. Finally, we successfully constructed a synthetic inducible promoter that increased the virus-inducing activity of other promoters using the baculovirus-inducible transcriptional activation region that binds to specific core elements of 39 K (i.e., spanning the region − 310 to − 355).ConclusionsIn summary, we constructed a novel, synthetic, and highly efficient biological tool, namely, a virus-inducible 39 K promoter, which provides endless possibilities for future research on gene function, gene therapy, and pest control in genetic engineering.Electronic supplementary materialThe online version of this article (10.1186/s13036-018-0121-8) contains supplementary material, which is available to authorized users.

show abstract

Construction of a novel synthetic root-specific promoter and its characterization in transgenic tobacco plants

Cited by 23 publications

References 43 publications

Dynamics of Global Gene Expression and Regulatory Elements in Growing Brachypodium Root System

Dynamics of Global Gene Expression and Regulatory Elements in Growing Brachypodium Root System

A Fruitful Decade Using Synthetic Promoters in the Improvement of Transgenic Plants

Construction and characterization of a synthetic Baculovirus-inducible 39K promoter

Contact Info

Product

Resources

About