Construction of a Test-System With Nanoparticles of Colloid Silver for Detection of Pseudotuberculosis and Intestinal Yersiniosis for Causative Agents in Dot-Immunoassay

Abstract

Aim. Construction of an immunologic test-system for detection of causative agents of entero-pathogenic yersinia {Yersinia pseudotuberculosis and Y. enterocolitica) by dot-immunoassay. Materials and methods. Nanoparticles of colloid silver sized 5 - 9 nm were used as a marker of specific antibodies. IgG fraction was isolated from commercial antisera to Y. pseudotuberculosis (0:1) and Y. enterocolitica (0:3 and 0:9). Testing of the obtained test-system was carried out on 20 strains of Y. pseudotuberculosis and Y. enterocolitica (10 of each species). Results. Dot-analysis had a specific character and detected enteropathogenic yersinia at a level of 5Т05 - 8T06 CFU/ml (100 - 1000 CFU in sample). Wherein cross-reaction with heterologic studied microorganisms - Escherichia coli, Salmonella typhimurium, Shigella flexneri, Yersiniapestis EV - was not observed. A possibility of simultaneous detection and serotyping of Y. enterocolitica is shown, that is necessary for confirmation of their epidemic significance. Conclusion. The developed test-systems allow to study micro volumes of the samples under study (1 pi), are express (1.5 - 2 h), highly sensitive and specific, technically simple and do not require the use of high-cost equipment, special training of the staff, may be successfully used in practical healthcare in laboratories with varying equipment levels.