2017
DOI: 10.3390/s17040676
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Construction of an Acetylcholinesterase Sensor Based on Synthesized Paramagnetic Nanoparticles, a Simple Tool for Neurotoxic Compounds Assay

Abstract: Magnetic particles (MPs) have been widely used in biological applications in recent years as a carrier for various molecules. Their big advantage is in repeated use of immobilized molecules including enzymes. Acetylcholinesterase (AChE) is an enzyme playing crucial role in neurotransmission and the enzyme is targeted by various molecules like Alzheimer’s drugs, pesticides and warfare agents. In this work, an electrochemical biosensor having AChE immobilized onto MPs and stabilized through glutaraldehyde (GA) m… Show more

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Cited by 13 publications
(8 citation statements)
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“…The use of the AChE immobilized on silica in microreactor at the inlet of capillary made it possible to detect HupA in plant extracts with the IC 50 of 1.52×10 −6 M . For GAL, IC 50 values varied from 0.47 (the AChE immobilized in polymetacrylate matrix) to 12.8 μM (enzyme cross‐linked with glutaraldehyde on silica fused tubing) . The electrochemical AChE biosensor with enzyme immobilized on magnetic particles and stabilized by glutaraldehyde treatment made it possible to detect Gal with LOD of 1.5 μM .…”
Section: Resultsmentioning
confidence: 99%
“…The use of the AChE immobilized on silica in microreactor at the inlet of capillary made it possible to detect HupA in plant extracts with the IC 50 of 1.52×10 −6 M . For GAL, IC 50 values varied from 0.47 (the AChE immobilized in polymetacrylate matrix) to 12.8 μM (enzyme cross‐linked with glutaraldehyde on silica fused tubing) . The electrochemical AChE biosensor with enzyme immobilized on magnetic particles and stabilized by glutaraldehyde treatment made it possible to detect Gal with LOD of 1.5 μM .…”
Section: Resultsmentioning
confidence: 99%
“…The comparison of their analytical characteristics is presented in Table S2 . Meanwhile, the only analog utilizing separate stages of enzyme inhibition and thiocholine detection [ 49 ] demonstrated a LOD of 20 nM and a general measurement time of more than 20 min.…”
Section: Resultsmentioning
confidence: 99%
“…The performance of the proposed sensor was compared with other sensors ( Table 1 ) in which AChE was immobilized on nanoparticles. Only the works of Jeyapragasam et al [ 12 ] and Kostelnik et al [ 20 ] involved the use of magnetic nanoparticles for pesticide determination. Although the pesticides listed in Table 1 are different, they all belong to the organophosphate or carbamate classes.…”
Section: Resultsmentioning
confidence: 99%
“…In the literature, usually, after the determination of pesticides, the electrodes are discarded, but in proposed methodology, the magnetic particles are washed away with water after taken the external magnet out, and then SPE is ready for another experiment. Although mNPs have been used for removal and quantification of rhodamine [ 16 ], as a glucose sensor [ 17 ], for the diagnosis of malaria [ 18 ], and for enzyme immunoassay atrazine sensor [ 19 ], concerning both the inhibition of AChE for pesticide detection and the use of iron magnetic particles for the AChE immobilization, only two articles were found in the literature [ 12 , 20 ].…”
Section: Introductionmentioning
confidence: 99%