Construction of an amperometric bilirubin biosensor based on covalent immobilization of bilirubin oxidase onto zirconia coated silica nanoparticles/chitosan hybrid film

Batra, Bhawna; Lata, Suman; Kumar, Suraj; Rana, J. S.; Pundir, C.S.

doi:10.1016/j.bios.2012.12.034

Cited by 89 publications

(40 citation statements)

References 17 publications

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“…High concentrations of unconjugated free BR in neonates can lead to Kernicterus (brain damage and hearing loss) and even death. 6 Low level of BR concentration is associated with anaemia and coronary artery diseases. 3 Neonatal jaundice is extremely common as almost every newborn infant child develops an unconjugated BR level greater than 340 mM L À1 during the rst week of life, generally considered as dangerous.…”

Section: Introductionmentioning

confidence: 99%

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Non-enzymatic detection of bilirubin based on a graphene–polystyrene sulfonate composite

Balamurugan

Berchmans

2015

RSC Adv.

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We report, a selective, non-enzymatic bilirubin (BR) detection through catalytic oxidation on a reduced graphene oxide (RGO)-poly styrene sulfonate (PSS) composite film spin coated on a glassy carbon electrode. The catalytic ability, stability, morphology and composition of the RGO-PSS composite, were evaluated using voltammetric, spectroscopic and microscopic techniques. The experimental variables influencing the analysis of bilirubin were optimized in terms of active material composition, pH and serum albumin concentration in blood. At the optimized condition, the linear dynamic range was found to be 0-450 mM, with a sensitivity of 0.16 mA mM À1 cm À2 and the detection limit was 2.0 mM. The modified electrode possesses good stability, reproducibility and selectivity towards bilirubin determination. The real sample analysis demonstrates the scope of the RGO-PSS composite modified electrode for practical clinical diagnosis.

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Section: Introductionmentioning

confidence: 99%

“…The normal level of direct bilirubin is 0-0.3 mg dL À1 and total bilirubin is 0.3 (5.1 mM) to 1.9 mg dL À1 (34.2 mM). 6 Various methods have been developed to detect BR. The most common spectroscopic measurement of bilirubin suffers due to interference from other proteins.…”

Section: Introductionmentioning

confidence: 99%

Non-enzymatic detection of bilirubin based on a graphene–polystyrene sulfonate composite

Balamurugan

Berchmans

2015

RSC Adv.

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“…There are a number of methods used for a stable immobilization, but the most used procedures are performed by physical adsorption [14], gel occlusion [15], a microencapsulation [16] and covalent bond [17] or covalent crosslink [18]. All these methods present many variantsand the choice of the most appropriate depends oneach particular case, considering the type of desired application, format and stability of the final device.…”

Section: Biosensorsmentioning

confidence: 99%

Applications and Perspectives of Biosensors for Diagnostics in Infectious Diseases

Etchegaray¹

2018

CJMI

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Infectious diseases are considered a major cause of morbidity and/or mortality worldwide, despite the development of preventive and control strategies. Infectious diseases result from the invasion of body tissues by disease-causing microorganisms. The occurrence and development of infectious diseases are closely associated with the functional state of the immune system. Interleukins play significant roles in modulating the immune response of the host during the course of an infectious disease, contributing to the maintenance of the homeostasis of the immune system. The use of biosensors for the detection of interleukins is a new and important strategy for the diagnosis of the infection and the immunological response profile that occurs for specific diseases, thus helping in the effective diagnosis, and allowing physicians to design treatment in a faster way, which contributes to better prognosis. For example, in the oral environment there are two major infectious diseases: caries and periodontitis. The microbes involved are respectively, Streptococcus mutans and Porphyromonasgingivalis. The presence of specific interleukins in saliva or crevicular fluid will indicate that there is potentially an infectious disease, which could be connected with either caries or periodontal disease. For instance, an indicative for caries could be the presence of IL-6 in saliva, while periodontal disease would be confirmed by the presence of IL-17 in crevicular fluid. In addition, both diseases could be confirmed by the detection of higher levels of each of one of the pathogens. Considering the development of biosensor design and construction, the answer to all these question could be obtained in a single analysis.

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“…In this context silica based hybrid materials containing chitosan have been developed mainly due to their potential applications for enzymes immobilization and for the development of electrochemical sensors [6][7][8]. The chitosan is considered an ideal support for immobilization of various electroactive species like proteins and enzymes for the preparation of electrochemical sensors and biosensors [9,10].…”

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confidence: 99%

Mesoporous chitosan/silica hybrid material applied for development of electrochemical sensor for paracetamol in presence of dopamine

Ramos

Morawski

Costa

et al. 2015

Microporous and Mesoporous Materials

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Construction of an amperometric bilirubin biosensor based on covalent immobilization of bilirubin oxidase onto zirconia coated silica nanoparticles/chitosan hybrid film

Cited by 89 publications

References 17 publications

Non-enzymatic detection of bilirubin based on a graphene–polystyrene sulfonate composite

Non-enzymatic detection of bilirubin based on a graphene–polystyrene sulfonate composite

Applications and Perspectives of Biosensors for Diagnostics in Infectious Diseases

Mesoporous chitosan/silica hybrid material applied for development of electrochemical sensor for paracetamol in presence of dopamine

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