Construction of Cell–Extracellular Matrix Microenvironments by Conjugating ECM Proteins on Supported Lipid Bilayers

Abstract

The cell membrane is an organized and fluid structure that modulates cellular activities in response to specific extracellular signals, and maintains the critical communication, integration, and homeostasis between the cytosol and the extracellular matrix (ECM). In recent years, tissue engineering and cell biology research has been rapidly progressed by a remarkable understanding of cell and ECM interfaces. In this review, the design of new biomimetic platforms based on the conjugation of ECM proteins on solid supported lipid bilayers (SLBs) will be summarized. The platforms provide a better system to evaluate cellular responses to specific recognition events, gradient, mechanical property, nanostructures, and inter-and intra-molecular interactions of ECM proteins on a non-fouling and fluid membrane. Moreover, the findings from the molecular interactions and cellular activities will be highlighted to look into the cell-materials mechanisms.

In vitro vascularized tumor platform for modeling tumor‐vasculature interactions of inflammatory breast cancer

“…Our lab previously established a 3D vascularized microfluidic breast cancer platform incorporated with MDA‐MB‐231 cells and a vascularized endothelial vessel that addressed the limitation described earlier with existing in vitro tumor models. Using this vascularized platform, we determined the relationship between wall shear stress (WSS) and signaling between cancer and endothelial cells on the vasculature (Buchanan, Verbridge, Vlachos, & Rylander, 2014; Gadde, Marrinan, Michna, & Rylander, 2018; Michna, Gadde, Ozkan, DeWitt, & Rylander, 2018) but similar to other non‐IBC in vitro tumor models the platform does not account for the complex tumor dynamics inherent to IBC (Bersini et al, 2014; Buchanan et al, 2012; Buchanan, Verbridge, et al, 2014; Buchanan, Voigt, et al, 2014; Duinen et al, 2017; Gadde et al, 2018; Ghousifam, Eftekharjoo, Derakhshan, & Gappa‐Fahlenkamp, 2019; Huang & Chang, 2019; Jeon et al, 2015; Kim et al, 2015; Kim, Chung, Ahn, Lee, & Li Jeon, 2016; Ko et al, 2019; Koh, Stratman, Sacharidou, & Davis, 2008; Ma, Middleton, You, & Sun, 2018; Malandrino, Kamm, & Moeendarbary, 2018; Meer, Orlova, Dijke, Berg, & Mummery, 2013; Michna et al, 2018; Nguyen et al, 2013; Osaki, Serrano, & Kamm, 2018; Ozcelikkale, Moon, Linnes, & Han, 2017; Pagano et al, 2014; Pouliot, Pearson, & Burrows, 2013; Pradhan et al, 2018; Rhodes & Simons, 2007; Shang, Soon, Lim, Khoo, & Han, 2019; Sleeboom, Eslami Amirabadi, Nair, Sahlgren, & den Toonder, 2018; Sontheimer‐Phelps, Hassell, & Ingber, 2019; Szot, Buchanan, Freeman, & Rylander, 2011; Szot, Buchanan, Freeman, & Rylander, 2013; Tsai, Trubelja, Shen, & Bao, 2017; Vickerman, Blundo, Chung, & Kamm, 2008).…”

Reversible Self-Assembled Monolayers with Tunable Surface Dynamics for Controlling Cell Adhesion Behavior

“…Integrins, the main cellular receptors for the extracellular matrix, have a key role in mediating these activities. , The tripeptide Arg-Gly-Asp or RGD is one of the highly conserved peptide sequences present in the extracellular matrix (ECM) recognized by the integrins. Since its discovery, this peptide sequence and its variations have been integrated into and onto a variety of scaffolds to investigate the role of cell adhesion molecules during cell adhesion processes and fabrication of biomaterials for cell culture, tissue engineering, and regenerative medicine. − The scaffolds for immobilizing bioactive peptides can be either static (biopolymers, self-assembled monolayers (SAMs) − ) or dynamic (hydrogels, , supported lipid bilayers (SLBs), host–guest-based assemblies, − self-assembled peptide amphiphiles). In terms of two-dimensional (2D) crystalline-like layers, the most well-studied cases are SAMs and SLBs. ,,,, The former in combination with light-responsive, , magnetic, or electrical-responsive functionalities, ,, or host–guest-based chemistry, − enable controllable surface properties for reversible cell adhesion albeit featuring only short-range dynamic properties.…”

“…Since its discovery, this peptide sequence and its variations have been integrated into and onto a variety of scaffolds to investigate the role of cell adhesion molecules during cell adhesion processes and fabrication of biomaterials for cell culture, tissue engineering, and regenerative medicine. − The scaffolds for immobilizing bioactive peptides can be either static (biopolymers, self-assembled monolayers (SAMs) − ) or dynamic (hydrogels, , supported lipid bilayers (SLBs), host–guest-based assemblies, − self-assembled peptide amphiphiles). In terms of two-dimensional (2D) crystalline-like layers, the most well-studied cases are SAMs and SLBs. ,,,, The former in combination with light-responsive, , magnetic, or electrical-responsive functionalities, ,, or host–guest-based chemistry, − enable controllable surface properties for reversible cell adhesion albeit featuring only short-range dynamic properties. The latter, however, are characterized by their long-range lateral mobility, which is conducive to integrin clustering required for downstream signal transduction paths for cell growth and differentiation .…”

“…The latter, however, are characterized by their long-range lateral mobility, which is conducive to integrin clustering required for downstream signal transduction paths for cell growth and differentiation . These systems have recently gained attention for their tunable lateral dynamics in investigating cell adhesion behavior and differentiation in the absence of elastic components in static architectures. ,, The downsides of SLBs as platforms for cell culture are their poor long-term stability, limited stability toward air exposure, and lack of stimuli responsiveness. , As such, modulating cell adhesion behavior followed by subsequent cell release using 2D crystalline-like platforms is important for cell-based applications but an extreme challenge for material scientists.…”

“…Since its discovery, this peptide sequence and its variations have been integrated into and onto a variety of scaffolds to investigate the role of cell adhesion molecules during cell adhesion processes and fabrication of biomaterials for cell culture, tissue engineering, and regenerative medicine. 7−14 The scaffolds for immobilizing bioactive peptides can be either static (biopolymers, 15 self-assembled monolayers (SAMs) 16−18 ) or dynamic (hydrogels, 19,20 supported lipid bilayers (SLBs), 11 host−guest-based assemblies, 19−22 self-assembled peptide amphiphiles 23 ). In terms of two-dimensional (2D) crystalline-like layers, the most well-studied cases are SAMs and SLBs.…”