Construction of chimeric plasmids containing histone H5 cDNA from hen erythrocyte. DNA sequence of a fragment derived from the 5′ region of H5 mRNA

Ruiz‐Vázquez, Rosa M.; Ruiz-Carrillo, Adolfo

doi:10.1093/nar/10.6.2093

Cited by 22 publications

(11 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 shows that the H5 probe detected a single RNA species with a length of 900 + 50 bases. This estimate is somewhat less than the 1100 bases obtained6, using polyacylamide gels run in formamide6, 20 Wallis16, and 9 positively-reacting clones were detected. As the cDNA library had been prepared from nRNA estimated to be 4-fold enriched for H5 mRNA, the number of H5 clones isolated suggests that H5 mRNA represents about 0.2% of chicken reticulocyte polyadenylated RNA.…”

Section: Resultsmentioning

confidence: 72%

Chicken histone H5 mRNA: the polyadenylated RNA lacks the conserved histone 3′ terminator sequence

et al. 1982

View full text Add to dashboard Cite

Using 3 overlapping cDNA clones we have determined the nucleotide sequence of chicken histonq H5 mRNA. The mRNA does not contain the 23 base conserved sequence element' that is present at the 3' end of cell-cycle regulated histone mRNAs. Although the RNA is polyadenylated it lacks the 3' AAUAAA sequence. I NTRODUCT IONHistones Hl and H5 share common structural features2 and occupy similar positions, outside the nucleosome, in chromatin structure3 Observed similarities between the primary sequences of the HI and H5 proteins have led to the suggestion that the genes coding for Hl and H5 may be related4.However, a number of observations indicate that Hl and H5 may not be as closely related as the protein sequence data would imply. Unlike the majority of the histone rRNAs, which lack a poly-A tail5, H5 mRNA has been shown to be polyadenylated6, and while H5 may be synthesized in the nondividing reticulocyte7, the expression of the major classes of histones, including Hl, appears to be closely coupled to DNA synthesis8'9.In this paper we present the nucleotide sequence of H5 mRNA. The determination of the structure of H5 mRNA and the analysis of features of the nucleotide sequence should help to clarify the relationship of H5 to the other cell-cycle regulated histone genes. MATERIALS AND METHODSSizing of H5 mRNA. Total chick embryo RNA (20 ,g), prepared by the guanidine-HCl extraction procedure10, was electrophoresed in the presence of 10 mM methyl mercury hydroxide in 1.5% agarose gels, as described by Bailey and Davidson1. The gels were treated with mercaptoethanol, stained C I R L Press Limited, Oxford, England. 6777

show abstract

Section: Resultsmentioning

confidence: 72%

Chicken histone H5 mRNA: the polyadenylated RNA lacks the conserved histone 3′ terminator sequence

et al. 1982

View full text Add to dashboard Cite

show abstract

“…An endonuclease activity specific for these homopolymer tracts would produce two unique DNA fragments. In the initial experiments we used plasmid p554 DNA (see Figure IB) containing a histone H5 cDNA (Ruiz-Vazquez and Ruiz-Carrillo, 1982) cloned at the PstI site of pBR322 by oligo(dG)/(dC) tailing (Lobban et al, 1973). The length of the homopolymer at the 5' and 3' ends of the cDNA is 24 and 17 respectively.…”

Section: Identification Of Endonuclease G Activity In Nuclear Extractsmentioning

confidence: 99%

Endonuclease G: a (dG)n X (dC)n-specific DNase from higher eukaryotes.

Ruiz-Carrillo¹,

Renaud²

1987

The EMBO Journal

110

View full text Add to dashboard Cite

An endonuclease activity (termed endonuclease G) that selectively cleaves DNA at (dG)". (dC). tracts has been partially purified from immature chicken erythrocyte nuclei. Sites where n 2 9 are cleaved in a manner that resembles types II and HI restriction nucleases. The nicking rate of the Gstrand is 4to 10-fold higher than that of the C-strand depending on the length of the (dG). -(dC). tract and/or nucleotide composition of the flanking sequences. Endonuclease G hydrolyzes (dG)24*(dC)24 of supercoiled DNA in a bimodal way every 9-11 nucleotides, the maxima in one strand corresponding to minima in the opposite, suggesting that it binds preferentially to one side of the double helix. The nuclease produces 5' phosphomonoester ends and its activity is dependent on Mg2+ or Mn2+. The wide distribution and high relative activity of endonuclease G in a variety of tissues and species argues for a general role of the enzyme. The striking correlation between genetic instability and poly(dG) -poly(dC) tracts in DNA suggests that these sequences and endonuclease G are involved in recombination processes.

show abstract

“…In the chicken, H5 is an early marker of the erythroid lineage. Thus, it is already expressed in avian erythroblastosis virus (AEV)-transformed cells arrested at the colony-forming unit [CFU-(E)] stage, when globin is not detected (5,44). Studies with transformed CFU-(E) cells have shown that the single-copy H5 gene (26,39) is transcribed constitutively in the different phases of the cell cycle (14; unpublished observations).…”

mentioning

confidence: 99%

Regulation of histone and beta A-globin gene expression during differentiation of chicken erythroid cells.

Affolter¹,

Côté²,

Renaud³

et al. 1987

Mol. Cell. Biol.

View full text Add to dashboard Cite

The expression of the genes for several histones and pA-globin was examined in the chicken erythroid cell lineage. During the transition from CFU-(E) to the mature erythrocyte, histone H5 gradually increased fourfold in nuclei with little concomitant displacement of the Hi histones. This resulted in a 70% net increase in linker histone (Hi plus H5) content. The differential accumulation of H5 reflected (i) an increase in the transcriptional activity of the H5 gene occurring at the erythroblast stage, (ii) an apparent longer half-life of H5 mRNA, and (iii) a higher stability of the protein. Although the transcriptional activity of the histone genes (except H5) decreased with cell age, it was not tightly coupled to the S phase. On the other hand, the mRNA levels for these histones were tightly regulated during the cell cycle. Use of protein and DNA synthesis inhibitors indicated that the content of H5 mRNA was regulated at the posttranscriptional level by a control mechanism(s) differing from those for the other histones. Although the transcription rates of the H5 and pA_globin genes were comparable, differential accumulation of iA_globin mRNA led to a 30-to 170-fold-higher copy number of the pA-globin mRNA as the cell matured.

show abstract

Construction of chimeric plasmids containing histone H5 cDNA from hen erythrocyte. DNA sequence of a fragment derived from the 5′ region of H5 mRNA

Cited by 22 publications

References 47 publications

Chicken histone H5 mRNA: the polyadenylated RNA lacks the conserved histone 3′ terminator sequence

Chicken histone H5 mRNA: the polyadenylated RNA lacks the conserved histone 3′ terminator sequence

Endonuclease G: a (dG)n X (dC)n-specific DNase from higher eukaryotes.

Regulation of histone and beta A-globin gene expression during differentiation of chicken erythroid cells.

Contact Info

Product

Resources

About