Construction of core-shell hybrid nanoparticles templated by virus-like particles

Liu, A.; Yang, Liulin; Verwegen, Martijn; Reardon, Damien; Cornelissen, Jeroen J. L. M.

doi:10.1039/c7ra11310b

Cited by 7 publications

(5 citation statements)

References 80 publications

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“…DPV measurements were done in the presence of 1 mM [Fe(CN) 6 ] −3/−4 in phosphate Buffer (PS) buffer in the potential window −0.3 V to+0.3 V at a scan rate 50mVs 1. The frequency range used for impedance measurement was 100 kHz to 1 Hz of [Fe (CN) 6 ] −3/−4 in PBS pH = 7.4 17 . Analysis of EIS spectra was done using equivalent circuits using ZSimpWin 3.22 (Princeton Applied Research) and results reported in Nyquist plots.…”

Section: Methodsmentioning

confidence: 99%

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Impediometric Electrochemical Sensor Based on The Inspiration of Carnation Italian Ringspot Virus Structure to Detect an Attommolar of miR

Ghazizadeh

Moosavifard

Daneshmand

et al. 2020

Sci Rep

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electrochemical sensors are the tools to detect the accurate and sensitive miRs. there is the challenge to increase the power and sensitivity of the surface for the electrochemical sensor. We design a viruslike hallow structure of cuco 2 o 4 that it holds the large amounts of p19 protein by mimicking of inherent virus (Carnation italian ringspot virus) to detect 21mir with the limit of detection (LOD = 1aM). The electrochemical measurements are performed between the potentials at −0.3 V and +0.3 V with 1 mM [fe(cn) 6 ] −3/−4. After dropping the cuco 2 o 4 on the Scpe (screen carbon printed electrode), the sensor is turned on due to the high electrochemical properties. Then, p19 proteins move into the hallow structure and inhibit the exchange of electrochemical reactions between the shells and the sensor is turned off. Then, adding the duplexes of RNA/miRs cause to increase the electrochemical property of p19 due to the change of p19 conformation and the system is turned on, again. So, for the first time, a virus-like hallow structure has been used to detect the 21miR in the human serum, MCF-7, Hella cells, with high sensitivity, specificity, and reproducibility in few minutes.

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Section: Methodsmentioning

confidence: 99%

“…After adding 0.3 mL of 99% ethanol, the slurry was washed of the bound RNA to remove the remaining proteins and other impurities. The purified total miRNA was then eluted in 60 μL RNase free water 17 .…”

Section: Identification Of the Specificity And Sensitivity Of The Cucmentioning

confidence: 99%

Impediometric Electrochemical Sensor Based on The Inspiration of Carnation Italian Ringspot Virus Structure to Detect an Attommolar of miR

Ghazizadeh

Moosavifard

Daneshmand

et al. 2020

Sci Rep

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“…This is important to mask the cytotoxicity of metals but also for pathogens such as viruses. In fact, silica–virus hybrid nanoparticles have been developed and this combination showed promise in gene delivery by enhancing the efficiency while reducing liver accumulation and immunogenicity . Although these results are encouraging, an often-insurmountable obstacle is the challenging removal of the silica coating, which involves toxic and corrosive hydrofluoric acid- or base-catalyzed hydrolysis, which is not suitable for in vivo applications .…”

Section: Introductionmentioning

confidence: 99%

pH-Responsive Silica Coatings: A Generic Approach for Smart Protection of Colloidal Nanoparticles

Lima

Avó

Berberan‐Santos

et al. 2022

ACS Appl. Nano Mater.

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The silica coating of nanoparticles and of complex bioentities such as viruses has gathered significant scientific interest due to the versatility of silica-based materials. These nanostructured core–shell architectures have emerged as a tool for drug delivery and biospecimen preservation. Yet, the absence of straightforward methods for removing the silica coating limits the applicability of this class of functional materials. For several drug delivery applications as well as for fast dissolution of silica nanomaterials, an acidic environment is frequently used. Acid-sensitive silica nanoparticles are extensively explored, but easily prepared acid-sensitive silica coatings are rare. In this context, we herein report a simple approach for coating polystyrene (PS) nanoparticles as model systems with a pH-responsive silica framework. The core–shell pH-responsive silica coatings were constructed by a seeded sol–gel route using tetraethyl orthosilicate (TEOS) and a pH-sensitive diimine organosilane. Carboxylate-modified PS beads with 100 nm were successfully used for the coating process, and the thickness of the silica shell was tuned between 7 and 20 nm by adjusting the TEOS concentration. The introduction of the diimine organosilane in the silica framework resulted in PS@SiO2* core–shell nanoparticles with raspberry-like shells instead of a smooth and homogeneous morphology. According to the mechanism, this was due to a combination of the surface charge of silica colloids and the rapid self-condensation of the organosilane moieties. When this acid-sensitive silica framework was exposed to an acidic environment (pH 5.0), the consequent hydrolysis of the imine bonds promoted a fast degradation of the silica coating, exposing the bare PS nanoparticles. The described results provide proof-of-concept evidence that pH-responsive silica coatings are a promising platform for a range of applications in drug delivery in an acidic environment, sensing, and as a protective synthetic coating that could be removed on demand.

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“…Protein-based compartments are intimately involved in cellular functions, where they serve as storage vessels, , assist in protein folding and protein degradation, and in some cases contain enzymatic reactions . Viruses provide an excellent example of the importance of protein cages in the packaging, transport, and delivery of biological cargoes. , Inspired by Nature, there is great interest in re-engineering natural protein cages toward a variety of applications: − these include targeted drug delivery, ,, polyvalent display of antigens, in vivo imaging, templating of nanoparticles, ,, and encapsulation of enzymes in protein nanoreactors. − …”

Section: Introductionmentioning

confidence: 99%

“…5 Viruses provide an excellent example of the importance of protein cages in the packaging, transport, and delivery of biological cargoes. 6,7 Inspired by Nature, there is great interest in re-engineering natural protein cages toward a variety of applications: 5−10 these include targeted drug delivery, 7,11,12 polyvalent display of antigens, 9 in vivo imaging, 13 templating of nanoparticles, 10,14,15 and encapsulation of enzymes in protein nanoreactors. 16−18 The protein cages most commonly used for such applications are virus-like particles 6 (VLPs) derived from icosahedral viruses.…”

Section: ■ Introductionmentioning

confidence: 99%

Coiled-Coil-Mediated Assembly of an Icosahedral Protein Cage with Extremely High Thermal and Chemical Stability

et al. 2019

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The organization of protein molecules into higher-order nanoscale architectures is ubiquitous in Nature and represents an important goal in synthetic biology. Here we describe the symmetrydirected design of a hollow protein cage with dimensions similar to those of many icosahedral viruses. The cage was constructed based on icosahedral symmetry by genetically fusing a trimeric protein (TriEst) to a small pentameric de novo-designed coiled coil domain, separated by a flexible oligo-glycine linker sequence. Screening a small library of designs in which the linker length varied from 2 to 12 residues identified a construct containing 8 glycine residues (Ico8) that formed well-defined cages. Characterization by dynamic light scattering, negative stain and cryo EM, and by atomic force and IR-photo-induced force microscopy established that Ico8 assembles into a flexible hollow cage with comprising 60-subunits with overall icosahedral geometry. Unexpectedly, the cages were found to encapsulate DNA, even though neither protein component binds nucleic acids on its own. Notably, the cages formed by Ico8 proved to be extremely stable towards thermal and chemical denaturation: whereas TriEst was unfolded by heating (T m ~75 ºC) or denatured by 1.5 M guanidine hydrochloride, the Ico8 cages remained folded even at 120 ºC or in 8 M guanidine hydrochloride. The encapsulation of DNA and increased stability of the cages are new properties that emerge from the higher order structure of the protein cage, rather than being intrinsic to the components from which it is constructed.

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Construction of core-shell hybrid nanoparticles templated by virus-like particles

Abstract: Catalytically active gold in silica core–shell nanoparticles are prepared by pH controlled templating on virus-like particles.

Cited by 7 publications

References 80 publications

Impediometric Electrochemical Sensor Based on The Inspiration of Carnation Italian Ringspot Virus Structure to Detect an Attommolar of miR

Impediometric Electrochemical Sensor Based on The Inspiration of Carnation Italian Ringspot Virus Structure to Detect an Attommolar of miR

pH-Responsive Silica Coatings: A Generic Approach for Smart Protection of Colloidal Nanoparticles

Coiled-Coil-Mediated Assembly of an Icosahedral Protein Cage with Extremely High Thermal and Chemical Stability

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